LOCUS       Exported                6987 bp ds-DNA     circular SYN 02-FEB-2024
DEFINITION  Generated by Gibson/Homology Cloning (oEL171 + oEL173, oEL172 + 
            oEL159, fEL042).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..1504
                     /label=Extracted region from EML001 with UNS
     primer_bind     complement(143..173)
                     /label=remove_amp_R
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1137..1158
                     /label=remove_amp_F
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4107
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     misc_feature    join(1505..6987,1..1504)
                     /label=TT
     CDS             1505..1561
                     /label=GmR
     primer_bind     1505..1526
                     /label=strep_cassette_F
     misc_feature    1508..2350
                     /label=AGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTC...
     CDS             1562..2353
                     /label=SmR
     primer_bind     complement(1902..1923)
                     /label=oEL254
     primer_bind     complement(2330..2353)
                     /label=strep_cassette_R
     primer_bind     2354..2380
                     /label=backbone piece_F
     terminator      2732..2763
                     /label=T4-32
     primer_bind     3691..3710
                     /label=oEL178
     terminator      3787..3818
                     /label=T4-32
     promoter        complement(3922..3999)
                     /label=AmpR promoter
     misc_feature    4075..5045
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    4075..4107
                     /label=Ligation
     primer_bind     complement(4080..4099)
                     /label=oEL182
     primer_bind     complement(4086..4107)
                     /label=3G_bb_R
     primer_bind     complement(4087..4107)
                     /label=oEL159
     misc_feature    4108..5045
                     /label=Extracted region from fEL042
     misc_feature    4108..4147
                     /label=UNS1
     primer_bind     4108..4146
                     /label=UNS1_F
     primer_bind     complement(4108..4146)
                     /label=UNS1_R
     misc_feature    4976..5015
                     /label=UNSX
     primer_bind     4976..5015
                     /label=UNSX_F
     misc_feature    5016..8491
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    5016..5045
                     /label=Ligation
     primer_bind     5016..5037
                     /label=oEL171
     misc_feature    5046..6987
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(5096..5294)
                     /label=transposon:Tn7
     primer_bind     complement(5303..5324)
                     /label=oEL188
     primer_bind     5304..5323
                     /label=oEL181
     rep_origin      complement(5376..5764)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             5761..5790
                     /label=TcR
     CDS             complement(5935..6306)
                     /label=traJ
     oriT            complement(6339..6448)
                     /direction=LEFT
     primer_bind     complement(6563..6581)
                     /label=remove_amp_and_ori_R
     rep_origin      complement(6582..6987)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     1561 aatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     1621 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     1681 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     1741 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     1801 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     1861 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     1921 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     1981 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2041 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2101 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2161 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2221 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     2281 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     2341 agtcggcaaa taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     2401 ttgacgggac ggcggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     2461 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
     2521 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
     2581 ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc
     2641 cccccccccc ctgcaggtcg actagagtcg gggatccggt gattgattga gcaagcttta
     2701 tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct ctagggtccc
     2761 caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac cggatcaatt
     2821 cccctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc gatccttgga
     2881 gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag
     2941 ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga agctgggcga acaaacgatg
     3001 ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa
     3061 gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg tgcacagcac
     3121 cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt
     3181 gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg
     3241 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     3301 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     3361 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     3421 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     3481 gtgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     3541 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     3601 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt
     3661 ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa tctagcgagg
     3721 gctttactaa gctgatccgg tggatgacct tttgaatgac ctttaataga ttatattact
     3781 aattaattgg ggaccctaga ggtccccttt tttattttaa aaattttttc acaaaacggt
     3841 ttacaagcat aaagcttgct caatcaatca ccggatcccg acgggcccgg taccgcggcc
     3901 gcgcgaattc gagctcggta ccatttatca gggttattgt ctcatgagcg gatacatatt
     3961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4021 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4081 gaggcccttt cgtcttcacc tcgagaacat tactcgcatc cattctcagg ctgtctcgtc
     4141 tcgtctcaat ttatcaaaaa gagtgttgac ttgacggcta gctcagtcct aggtacagtg
     4201 ctagctcaca catctagaat taaagaggag aaattaagca tgcgtaaagg agaagaactt
     4261 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     4321 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     4381 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
     4441 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
     4501 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     4561 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     4621 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     4681 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     4741 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     4801 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     4861 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     4921 gggattacac atggcatgga tgaactatac aaataagctt aattagctga gcttgccagg
     4981 atacatagat taccacaact ccgagccctt ccaccccaag ctagcttgga ggacaaatcc
     5041 gccgctagga gcttgcggcc gccttaagac gcgtggatcc ccgggaattt cgaccccacg
     5101 cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg ctttagccat
     5161 aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt tacaactatt
     5221 ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt ttaagacttt
     5281 attgtccgcc cacacccgct tacgcagggc atccatttat tactcaaccg taaccgattt
     5341 tgccaggtta cgcggctggt cctctagaat tcccatgtca gccgttaagt gttcctgtgt
     5401 cactcaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt
     5461 tgtccacaac cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat
     5521 aaaacttaaa accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa
     5581 catgagagct tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac
     5641 gttagccatg agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct
     5701 tagtacgtga aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcttca
     5761 gatcctctac gccggacgca tcgtggccgg atcggggggg gggggcgctc ggtcttgcct
     5821 tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg
     5881 gggacgtgct tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg
     5941 ctctgccctc gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc
     6001 gatcttcgcc agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc
     6061 ggtgagccag agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag
     6121 ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag
     6181 caggtaggcc gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc
     6241 gtctggaagg cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc
     6301 agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg
     6361 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     6421 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
     6481 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     6541 aatcgctata atgaccccga agcagggtta tgcagcggaa aagcgctttc tcatagctca
     6601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6961 attacgcgca gaaaaaaagg atctcaa
//
