LOCUS       238408-attachmen        6952 bp DNA     circular SYN 04-JUN-2025
DEFINITION  Generated by Gibson/Homology Cloning (oEL171 + oEL173, oEL172 + 
            oEL159, fEL042).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 4, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4835..4936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1502
                     /label=Extracted region from EML001 with UNS
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1473..4009
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1473..1502
                     /label=Ligation
     primer_bind     complement(1473..1502)
                     /label=oEL173
     primer_bind     1473..1502
                     /label=oEL172
     primer_bind     complement(1473..1502)
                     /label=oEL173
     primer_bind     1473..1502
                     /label=oEL172
     misc_feature    1505..2255
                     /label=Extracted region from pUC18-mini-Tn7T::pDawn-
     misc_feature    1505..2251
                     /label=ATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAA
                     /label=ATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAA...
     promoter        1505..1533
                     /label=Pc promoter
     primer_bind     1505..1526
                     /label=Gent_F
     CDS             1722..2255
                     /label=GmR
     primer_bind     complement(2238..2255)
                     /label=Gent_R
     terminator      2634..2665
                     /label=T4-32
     primer_bind     3593..3612
                     /label=oEL178
     terminator      3689..3720
                     /label=T4-32
     promoter        complement(3824..3901)
                     /label=AmpR promoter
     primer_bind     3927..3955
                     /label=oEL245
     misc_feature    3977..5010
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    3977..4009
                     /label=Ligation
     primer_bind     complement(3982..4001)
                     /label=oEL182
     primer_bind     complement(3988..4009)
                     /label=3G_bb_R
     primer_bind     complement(3989..4009)
                     /label=oEL159
     misc_feature    4010..5010
                     /label=Extracted region from fEL042
     misc_feature    4010..4049
                     /label=UNS1
     primer_bind     4010..4048
                     /label=UNS1_F
     primer_bind     complement(4010..4048)
                     /label=UNS1_R
     misc_feature    4054..4088
                     /label=J23100
     5'UTR           4097..4108
                     /label=B0034
     misc_feature    4835..4936
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    4835..4936
                     /label=Extracted region from fEL042
     misc_feature    4941..4980
                     /label=UNSX
     misc_feature    4981..6952
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    4981..5010
                     /label=Ligation
     primer_bind     4981..5002
                     /label=oEL171
     misc_feature    5011..6952
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(5061..5259)
                     /label=transposon:Tn7
     primer_bind     complement(5268..5289)
                     /label=oEL188
     primer_bind     5269..5288
                     /label=oEL181
     rep_origin      complement(5341..5729)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             5726..5755
                     /label=TcR
     CDS             complement(5900..6271)
                     /label=traJ
     oriT            complement(6304..6413)
                     /direction=LEFT
     rep_origin      complement(6547..6952)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1441 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     1501 tgttttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg
     1561 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     1621 ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     1681 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagcaacg
     1741 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca
     1801 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt
     1861 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc
     1921 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg
     1981 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct
     2041 atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca
     2101 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag
     2161 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga
     2221 tgcactttga tatcgaccca agtaccgcca aataatcaga attggttaat tggttgtaac
     2281 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     2341 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     2401 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     2461 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     2521 ttcacgaggc agacctcagc gccccccccc ccctgcaggt cgactagagt cggggatccg
     2581 gtgattgatt gagcaagctt tatgcttgta aaccgttttg tgaaaaaatt tttaaaataa
     2641 aaaaggggac ctctagggtc cccaattaat tagtaatata atctattaaa ggtcattcaa
     2701 aaggtcatcc accggatcaa ttcccctgct cgcgcaggct gggtgccaag ctctcgggta
     2761 acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga
     2821 aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc gttccataca
     2881 gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat
     2941 ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc
     3001 agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac
     3061 gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt
     3121 cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     3181 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     3241 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     3301 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     3361 gttacgccgt gggtcgatgt ttgtgtctaa caattcgttc aagccgacgc cgcttcgcgg
     3421 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     3481 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     3541 tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca
     3601 tccgcattaa aatctagcga gggctttact aagctgatcc ggtggatgac cttttgaatg
     3661 acctttaata gattatatta ctaattaatt ggggacccta gaggtcccct tttttatttt
     3721 aaaaattttt tcacaaaacg gtttacaagc ataaagcttg ctcaatcaat caccggatcc
     3781 cgacgggccc ggtaccgcgg ccgcgcgaat tcgagctcgg taccatttat cagggttatt
     3841 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3901 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3961 cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagaac attactcgca
     4021 tccattctca ggctgtctcg tctcgtctcg gagttgacgg ctagctcagt cctaggtaca
     4081 gtgctagcta ctagagaaag aggagaaata ctaaatgatg cgtaaaggag aagaactttt
     4141 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     4201 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     4261 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
     4321 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
     4381 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
     4441 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
     4501 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
     4561 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
     4621 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
     4681 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     4741 gaaagatccc aacgaaaaga gagatcacat ggtccttctt gagtttgtaa cagctgctgg
     4801 gattacacat ggcatggatg aactatacaa ataataaagg tgactcctgt tgatagatcc
     4861 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
     4921 ttttattggt gagaatgctt ccaggataca tagattacca caactccgag cccttccacc
     4981 ccaagctagc ttggaggaca aatccgccgc taggagcttg cggccgcctt aagacgcgtg
     5041 gatccccggg aatttcgacc ccacgcccct ctttaatacg acgggcaatt tgcacttcag
     5101 aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca cagcataact
     5161 ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc atagtttaga
     5221 tctattttgt tcagtttaag actttattgt ccgcccacac ccgcttacgc agggcatcca
     5281 tttattactc aaccgtaacc gattttgcca ggttacgcgg ctggtcctct agaattccca
     5341 tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc
     5401 tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag
     5461 ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg
     5521 atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag
     5581 tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga
     5641 gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat
     5701 caacaggttg aactgctgat cttcagatcc tctacgccgg acgcatcgtg gccggatcgg
     5761 gggggggggg cgctcggtct tgccttgctc gtcggtgatg tacttcacca gctccgcgaa
     5821 gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca ccccccggcc
     5881 gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc atcatgacct
     5941 tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc gtggcatcac
     6001 cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg cccaggcggc
     6061 ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg acgcccgtga
     6121 ttttgtagcc ctggccgacg gccagcaggt aggccgacag gctcatgccg gccgccgccg
     6181 ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata ggtgggctgc
     6241 ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg
     6301 tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
     6361 cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac
     6421 gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg
     6481 cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag
     6541 cggaaaagcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aa
//
