LOCUS       pAKN116_[pCargo_vInduci 9331 bp ds-DNA     circular     17-APR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          4..22
                     /label="V11-seq-1"
                     /note="sequence: cgacccttgtccttttccg"
     misc_feature    complement(17..258)
                     /label="full oriT"
     oriT            complement(150..258)
                     /label="incP origin of transfer"
     primer          complement(204..221)
                     /label="oRRG1468"
                     /note="sequence: gccaggtgcgaataaggg"
     rep_origin      complement(277..665)
                     /label="R6K gamma ori"
     primer          278..301
                     /label="oSW4"
                     /note="sequence: atctgaagatcagcagttcaacct"
     primer          complement(492..519)
                     /label="OLQ1132"
                     /note="sequence: aaacttaaaaccttagaggctatttaag"
     primer          580..599
                     /label="QB0082"
                     /note="sequence: AACAAGCCAGGGATGTAACG"
     primer          complement(644..665)
                     /label="oSW1"
                     /note="sequence: tgtcagccgttaagtgttcctg"
     misc_feature    680..1469
                     /label="cen4 piece"
     misc_feature    798..908
                     /label="cen4 core"
     primer          1044..1067
                     /label="oPNC980"
                     /note="sequence: gcgttattggtgttgatgtaagcg"
     primer          1061..1080
                     /label="OZL672"
                     /note="sequence: gtaagcggaggtgtggagac"
     primer          complement(1061..1080)
                     /label="OZL668"
                     /note="sequence: gtctccacacctccgcttac"
     primer          1265..1292
                     /label="oPNC572_CEN4-ARS_Seq_Dn"
                     /note="sequence: catttggctttttgattgattgtacagg"
     primer          complement(1302..1321)
                     /label="OZL654"
                     /note="sequence: AAAAGTCAACCCCCTGCGAT"
     primer          1377..1398
                     /label="PNC423_CEN4_Seq_Dn-1"
                     /note="sequence: cgctacaatgacccgattcttg"
     primer          1418..1437
                     /label="V12-seq-1"
                     /note="sequence: gctgctagcaccagagctac"
     misc_feature    1489..1522
                     /label="terminator Bba_B1004"
     primer          1494..1508
                     /label="oAKN224"
                     /note="sequence: cgcaaaaaaccccgcttcgg"
     misc_feature    1523..1542
                     /label="Spacer"
     misc_feature    complement(1543..2256)
                     /label="CI857 recoded"
     primer          1817..1836
                     /label="OLQ654"
                     /note="sequence: TCTACTTCCAGCCAAAACGC"
     primer          complement(1825..1841)
                     /label="OLQ653"
                     /note="sequence: ATAGCGCGTTTTGGCTG"
     misc_feature    complement(2257..2291)
                     /label="RBS 22614"
     misc_feature    2298..2303
                     /label="-10 pRM"
     misc_feature    2302..2318
                     /label="OR3"
     misc_feature    2321..2326
                     /label="-35 pRM"
     misc_feature    2325..2341
                     /label="OR2"
     misc_feature    2339..2345
                     /label="pR -35"
     misc_feature    2349..2365
                     /label="OR1"
     misc_feature    2362..2367
                     /label="pR -10"
     misc_feature    2374..2374
                     /label="TSS"
     misc_feature    2376..2427
                     /label="pcepA from IS1224"
     primer          2403..2454
                     /label="OLQ1087"
                     /note="sequence: tacctttgtggaccgtattacaaagAGAAGTGTTAAATCAAACAAAAAGGAG"
     primer          complement(2405..2427)
                     /label="OLQ1061"
                     /note="sequence: ctttgtaatacggtccacaaagg"
     misc_feature    2428..2462
                     /label="RBS BT_2162"
     misc_feature    2463..4280
                     /label="phiC31 integrase"
     primer          complement(2672..2694)
                     /label="BP0039"
                     /note="sequence: ATTCGTTCAGGATGCGTTCGAAC"
     primer          complement(2753..2772)
                     /label="BP0040"
                     /note="sequence: GAATCGCGTCCATGACCTTC"
     primer          3148..3164
                     /label="V11-seq-3"
                     /note="sequence: TTCCCTTCAAGCCGGGC"
     primer          4217..4231
                     /label="V11-seq-4"
                     /note="sequence: GAAGCCGCCGACCGA"
     primer          4230..4247
                     /label="OLQ595_phiC31"
                     /note="sequence: GACGACGACGAAGACGAC"
     promoter        complement(4281..4299)
                     /label="SP6 promoter"
     misc_feature    4300..4337
                     /label="attB site"
     primer          complement(4330..4355)
                     /label="oAKN197"
                     /note="sequence: ATATGTGGAGGTCTCaGTGTATTACCCTGTTATCCCTAtacgcgcc"
     primer          complement(4330..4375)
                     /label="oAKN197"
                     /note="sequence: ATATGTGGAGGTCTCaGTGTATTACCCTGTTATCCCTAtacgcgcc"
     primer          complement(4333..4352)
                     /label="OLQ655"
                     /note="sequence: ACCCTGTTATCCCTAtacgc"
     primer          complement(4333..4355)
                     /label="oAKN217"
                     /note="sequence: ATTACCCTGTTATCCCTAtacgc"
     primer          4336..4378
                     /label="oAKN198"
                     /note="sequence: taTAGGGATAACAGGGTAATACACtGAGACCTCCACATATACC"
     misc_feature    4338..4355
                     /label="I-sceI site"
     primer          4356..4378
                     /label="oAKN198"
                     /note="sequence: taTAGGGATAACAGGGTAATACACtGAGACCTCCACATATACC"
     misc_feature    4361..4366
                     /label="bsaI"
     misc_feature    4815..6236
                     /label="sacB"
     primer          6184..6204
                     /label="V12-seq-3"
                     /note="sequence: CTGTTGTCAAAGACAGCATCC"
     primer          6185..6205
                     /label="pMobv2sq-f"
                     /note="sequence: TGTTGTCAAAGACAGCATCCT"
     misc_feature    6237..6242
                     /label="bsaI"
     primer          6243..6262
                     /label="oAKN216"
                     /note="sequence: tAGAGccaccacagtggtta"
     primer          complement(6244..6262)
                     /label="OLQ1136"
                     /note="sequence: taaccactgtggtggCTCT"
     misc_feature    6248..6259
                     /label="bstX1"
     primer          6248..6269
                     /label="OLQ1060"
                     /note="sequence: ccaccacagtggttaattaaGG"
     primer          6248..6269
                     /label="oAKN194"
                     /note="sequence: ccaccacagtggttaattaaGG"
     primer          6248..6269
                     /label="OLQ1131"
                     /note="sequence: ccaccacagtggttaattaaGG"
     primer          6248..6269
                     /label="oAKN135"
                     /note="sequence: CGTCGCGCTGAAGGGTTCTAccaccacagtggttaattaaGG"
     primer          6248..6269
                     /label="oAKN082"
                     /note="sequence: gcgtttgatagttgagtcgaccaccacagtggttaattaaGG"
     primer          6248..6270
                     /label="oDAM003"
                     /note="sequence: ccaccacagtggttaattaaGGC"
     primer          6248..6271
                     /label="oAKN190"
                     /note="sequence: AACTGTCCGATGATGATTAAccaccacagtggttaattaaGGCC"
     misc_feature    6260..6267
                     /label="pacI"
     primer          6267..6285
                     /label="oAKN160"
                     /note="sequence: CGTTACGGTCTCGCAGGCCtgtgaGGCCGGACC"
     misc_feature    6268..6280
                     /label="sfiI"
     primer          6273..6288
                     /label="oAKN118"
                     /note="sequence: GtgaGGCCGGACCAAA"
     primer          6276..6292
                     /label="OLQ652"
                     /note="sequence: aGGCCGGACCAAAACGA"
     primer          complement(6276..6292)
                     /label="OLQ1137"
                     /note="sequence: TCGTTTTGGTCCGGCCt"
     primer          complement(6281..6301)
                     /label="pMobIns-sqr"
                     /note="sequence: GGCCTTTTTTCGTTTTGGTCC"
     misc_feature    complement(6281..6341)
                     /label="L3S2P21"
     primer          6307..6326
                     /label="OLQ082"
                     /note="sequence: TTCGGGAGGCCTCTTTTCTG"
     primer          6316..6339
                     /label="OLQ927"
                     /note="sequence: CCTCTTTTCTGGAATTTGGTACCG"
     primer          complement(6319..6341)
                     /label="OLQ837"
                     /note="sequence: CTCGGTACCAAATTCCAGAAAAG"
     primer          complement(6322..6341)
                     /label="oAKN199"
                     /note="sequence: attatgatagaatttgacgtCTCGGTACCAAATTCCAGAA"
     primer          complement(6322..6361)
                     /label="oAKN199"
                     /note="sequence: attatgatagaatttgacgtCTCGGTACCAAATTCCAGAA"
     primer          6322..6366
                     /label="oAKN200"
                     /note="sequence: TTCTGGAATTTGGTACCGAGacgtcaaattctatcataattgtgg"
     primer          6342..6366
                     /label="oAKN200"
                     /note="sequence: TTCTGGAATTTGGTACCGAGacgtcaaattctatcataattgtgg"
     primer          6342..6368
                     /label="OLQ1058"
                     /note="sequence: acgtcaaattctatcataattgtggtt"
     misc_feature    6342..6528
                     /label="pKanR from Enterococcus facealis"
     primer          complement(6377..6396)
                     /label="OLQ1073"
                     /note="sequence: aacatagtatcgacggagcc"
     primer          6496..6524
                     /label="oSW46"
                     /note="sequence: gcttcttggggtatctttaaatactgtag"
     misc_feature    6529..6576
                     /label="permF from IS4351f"
     primer          complement(6554..6576)
                     /label="OLQ651"
                     /note="sequence: TTGCAGATGAGCAAACATATAAC"
     primer          6577..6604
                     /label="PNC272_P-TEF_Up_F"
                     /note="sequence: GACATGGAGGCCCAGAATACCCTCCTTG"
     misc_feature    6577..6920
                     /label="tef promoter"
     primer          complement(6588..6610)
                     /label="PNC320_pTEF_Seq_Up"
                     /note="sequence: gactgtcaaggagggtattctgg"
     primer          6885..6906
                     /label="OLQ926"
                     /note="sequence: atacagttctcacatcacatcc"
     primer          complement(6888..6907)
                     /label="OLQ928"
                     /note="sequence: cggatgtgatgtgagaactg"
     misc_feature    6921..6950
                     /label="RBS of ermB"
     misc_feature    6951..7760
                     /label="kanR aph(3)Ia"
     primer          6992..7011
                     /label="OLQ337_kanR(OFF)-mid-r"
                     /note="sequence: ttccaacatggatgctgatt"
     primer          complement(7018..7037)
                     /label="OZL117"
                     /note="sequence: ATCGCGAGCCCATTTATACC"
     primer          complement(7033..7052)
                     /label="PNC011_aph_Seq_R"
                     /note="sequence: tgattgcccgacattatcgc"
     primer          complement(7050..7069)
                     /label="OLQ336_kanR(OFF)-mid-f"
                     /note="sequence: cgatagattgtcgcacctga"
     primer          7103..7123
                     /label="oRRG135"
                     /note="sequence: TCTGAAACATGGCAAAGGTAG"
     primer          7164..7183
                     /label="G0161"
                     /note="sequence: tggctgacggaatttatgcc"
     primer          7179..7198
                     /label="oSW59"
                     /note="sequence: ATGCCTCTTCCGACCATCAA"
     primer          complement(7332..7351)
                     /label="oSW60"
                     /note="sequence: ACAGGAATCGAATGCAACCG"
     misc_feature    7432..7432
                     /label="G-->T mutation from expected"
     primer          7614..7633
                     /label="OZL118"
                     /note="sequence: GACCGATACCAGGATCTTGC"
     primer          7629..7649
                     /label="DKSs131"
                     /note="sequence: cttgccatcctatggaactgc"
     primer          complement(7650..7673)
                     /label="G0162"
                     /note="sequence: taatgaaggagaaaactcaccgag"
     primer          7657..7681
                     /label="V12-seq-2"
                     /note="sequence: AGTTTTCTCCTTCATTACAGAAACG"
     primer          7661..7682
                     /label="PNC012_aph_Seq_F"
                     /note="sequence: ttctccttcattacagaaacgg"
     primer          complement(7669..7692)
                     /label="oRRG136"
                     /note="sequence: TTTGAAAAAGCCGTTTCTGTAATG"
     primer          7724..7746
                     /label="G0405"
                     /note="sequence: attgcagtttcatttgatgctcg"
     misc_feature    7766..7963
                     /label="TEF terminator"
     primer          7898..7919
                     /label="PNC319_tTEF_Seq_Dn"
                     /note="sequence: gcgaagttaagtgcgcagaaag"
     primer          7929..7951
                     /label="oPNC981"
                     /note="sequence: gcgtcaatcgtatgtgaatgctg"
     primer          complement(7930..7963)
                     /label="PNC273_P-TEF_Dn_R"
                     /note="sequence: CAGTATAGCGACCAGCATTCACATACGATTGACG"
     primer          7936..7955
                     /label="oAKN055"
                     /note="sequence: tcgtatgtgaatgctggtcg"
     misc_feature    7966..8236
                     /label="tZ terminatorhttp://pubs.acs.org/doi/10.1021/sb5000115"
     misc_feature    7975..8007
                     /label="T3 term"
     primer          complement(8027..8049)
                     /label="oRRG319"
                     /note="sequence: ACTGAGCCTTTCGTTTTATTTGA"
     primer          complement(8028..8050)
                     /label="PNC192_rrnBT1_Seq_R"
                     /note="sequence: gactgagcctttcgttttatttg"
     primer          8030..8050
                     /label="G0326"
                     /note="sequence: Aataaaacgaaaggctcagtc"
     primer          8031..8051
                     /label="oAKN184"
                     /note="sequence: TCACCAGGTCTCCACACataaaacgaaaggctcagtcg"
     primer          8033..8053
                     /label="G0117"
                     /note="sequence: aaaacgaaaggctcagtcgaa"
     misc_feature    8037..8068
                     /label="rrnB T1"
     primer          8108..8124
                     /label="oAKN058"
                     /note="sequence: TAATACGACTCACTATAGGGCCACCACCACTGGGATC"
     primer          complement(8124..8141)
                     /label="oRRG1015"
                     /note="sequence: ggctttgttagcagccgg"
     primer          complement(8129..8149)
                     /label="OEA067"
                     /note="sequence: TCCTTTCGGGCTTTGTTAGCA"
     primer          8161..8177
                     /label="OEA092"
                     /note="sequence: CTGCTGCCACCGCTGAG"
     primer          complement(8170..8188)
                     /label="BP0013"
                     /note="sequence: GCTAGTTATTGCTCAGCGG"
     primer          complement(8170..8188)
                     /label="genewiz_T7 Term"
                     /note="sequence: GCTAGTTATTGCTCAGCGG"
     primer          complement(8170..8188)
                     /label="QB0107"
                     /note="sequence: GCTAGTTATTGCTCAGCGG"
     misc_feature    8191..8226
                     /label="T7 terminator"
     primer          complement(8207..8226)
                     /label="BP0025"
                     /note="sequence: AACCCCTCAAGACCCGTTTA"
     primer          complement(8207..8227)
                     /label="OEA100"
                     /note="sequence: AAACCCCTCAAGACCCGTTTA"
     primer          complement(8212..8231)
                     /label="oAKN053"
                     /note="sequence: caggtcgactctagaggatccccCAAAAAACCCCTCAAGACCC"
     primer          complement(8217..8236)
                     /label="oAKN201"
                     /note="sequence: gccctggcacccCTCGGTACTTCAGCAAAAAACCCCTCAA"
     primer          8217..8254
                     /label="oAKN196"
                     /note="sequence: TTGAGGGGTTTTTTGCTGAAGTACCGAGgggtgccagg"
     primer          complement(8217..8256)
                     /label="oAKN201"
                     /note="sequence: gccctggcacccCTCGGTACTTCAGCAAAAAACCCCTCAA"
     misc_feature    complement(8237..8244)
                     /label="L3S2P21"
     primer          8237..8254
                     /label="oAKN196"
                     /note="sequence: TTGAGGGGTTTTTTGCTGAAGTACCGAGgggtgccagg"
     misc_feature    8245..8282
                     /label="attB site"
     primer          8293..8314
                     /label="OLQ969"
                     /note="sequence: aaattagtagcccgcctaatga"
     promoter        8339..8430
                     /label="AmpR promoter"
     primer          complement(8369..8388)
                     /label="OZL191"
                     /note="sequence: ttgtctcatgagcggataca"
     primer          8370..8393
                     /label="oRRG1476"
                     /note="sequence: GTATCCGCTCATGAGACAATAACC"
     primer          complement(8374..8395)
                     /label="OLQ025"
                     /note="sequence: AGGGTTATTGTCTCATGAGCGG"
     primer          complement(8375..8400)
                     /label="Pbla_Seq_Up"
                     /note="sequence: ttatcagggttattgtctcatgagcg"
     primer          complement(8379..8407)
                     /label="oRRG1477"
                     /note="sequence: GAAGCATTTATCAGGGTTATTGTCTCATG"
     primer          complement(8402..8430)
                     /label="CFH397"
                     /note="sequence: ACTCTTCCTTTTTCAATATTATTGAAGCA"
     primer          complement(8412..8430)
                     /label="QB0167"
                     /note="sequence: ACTCTTCCTTTTTCAATAT"
     CDS             8431..9291
                     /label="AmpR"
     primer          complement(8433..8456)
                     /label="oAKN077"
                     /note="sequence: GCAATTAACCCTCACTAAAGGccacacgaaaatgctgaatgctc"
     primer          complement(8500..8521)
                     /label="oSW38"
                     /note="sequence: ctttcaccagggtttccggatg"
     primer          9291..9312
                     /label="OLQ1173"
                     /note="sequence: aaccgatacaattaaaggctcc"
     primer          complement(9291..9312)
                     /label="OLQ1174"
                     /note="sequence: ggagcctttaattgtatcggtt"
     terminator      complement(9295..3)
                     /label="fd terminator"
ORIGIN
        1 ggacgaccct tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
       61 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
      121 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
      181 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
      241 gctctgcgag gctggccgta ggccggccgg cgcgccgatc tgaagatcag cagttcaacc
      301 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac
      361 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta
      421 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
      481 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
      541 taaggctttt aaagccttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
      601 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc
      661 tgacatgggg cgcgcccagg tcagtcacag tggagcctag cactcActca gcgtgacggc
      721 tcagagcaga attcacgagc cagaaatagt aacttttgcc taaatcacaa attgcaaaat
      781 ttaattgctt gcaaaaggtc acatgcttat aatcaacttt tttaaaaatt taaaatactt
      841 ttttattttt tatttttaaa cataaatgaa ataatttatt tattgtttat gattaccgaa
      901 acataaaacc tgctcaagaa aaagaaactg ttttgtcctt ggaaaaaaag cactacctag
      961 gagcggccaa aatgccggct tacattttat gttagctggt ggactgacgc cagaaaatgt
     1021 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac
     1081 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt
     1141 tattactgag tagtatttat ttaagtattg tttgtgcact tgcctgcaag ccttttgaaa
     1201 agcaagcata aaagatctaa acataaaatc tgtaaaataa caagatgtaa agataatgct
     1261 aaatcatttg gctttttgat tgattgtaca ggaaaatata catcgcaggg ggttgacttt
     1321 taccatttca ccgcaatgga attaaacttg ttgaagagaa tgttcacagg cgcatacgct
     1381 acaatgaccc gattcttgct agccgaattc cagtcaggct gctagcacca gagctacgtg
     1441 accgcaggac tagctccagc tgagcgacaG cggcaagaaa gccatccacg ccgaaaaccc
     1501 cgcttcggcg gggttttgcc gcATCTTGCC GTAACTGACA AATTAACCGA AGGTTTCTTC
     1561 CGGCCACTGG GATGCAATGA CCTTACCAAC AACGCTACAG GATTCGTTGC ACGGGATCAT
     1621 TGGGTATTGC GGATTCAGCG GCTGCAGGAA AACTTGGCCA GAGTCGCGGA TCAGCTTCTT
     1681 AAAGGTGAAT TCATCACCAC CCAGGCGCGC AATGCAAAAG TCTCCCGGCT CAACCGCCTG
     1741 CTCAGGATCA ACCAGGATCA GCATACCATC CGGAAATGAC GGCTTGCTGC CCGTCGGGGC
     1801 CGTCATGCTG TTGCCTTCTA CTTCCAGCCA AAACGCGCTA TCGCTCGCTT TTTTGGTAGT
     1861 CGATACCCAA CGCTCAGCGT CACCTTTGGT GAAGGTACGC AGTTcCGGGC TAAACATACC
     1921 TGCTTGAACG TGGCTGAAGA CCGGGTATTC GTATTCAGAG CGCAGAGACG GTTGCATGCT
     1981 AACCGCTTCA TACATTTCAT AGATTTCACG AGCTATAGAA GGAGAGAACT CCTCGACGGA
     2041 AACTTTCAGA ATCTTGGTCA GCAGTGCGGC GTTGTACGCG TTCAGCGCAT TAATACCGTT
     2101 AAACAGAGCG CCAACGCCGC TCTGACCCAT GCCCATCTTG TCGGCTACGC TTTCCTGAGA
     2161 CAGACCCAGC TCATTCTTTT TCTTCTCATA GATTGCTTTC AGACGACGTG CGTCCTCTAG
     2221 CTGCTCTTGT GTAAGCGGCT TTTTCTTCGT TGACATTTTG ATCCTCCTTT ATATGGAGGT
     2281 ACTAAATCGG AACGTTAAAT CTATCACCGC AAGGGATAAA TATCTAACAC CGTGCGTGTT
     2341 GACTATTTTA CCTCTGGCGG TGATAATGGT TGCATcaaat ttgcgcgcca cattattatt
     2401 catacctttg tggaccgtat tacaaagAGA AGTGTTAAAT CAAACAAAAA GGAGGATTAA
     2461 TCATGGACAC GTACGCGGGT GCTTACGACC GTCAGTCGCG CGAGCGCGAa AATTCGAGCG
     2521 CAGCAAGCCC AGCGACACAG CGTAGCGCCA ACGAAGACAA GGCGGCCGAC CTTCAGCGCG
     2581 AAGTCGAGCG CGACGGGGGC CGGTTCAGGT TCGTCGGGCA TTTCAGCGAA GCGCCGGGCA
     2641 CGTCGGCGTT CGGGACGGCG GAGCGCCCGG AGTTCGAACG CATCCTGAAC GAATGCCGCG
     2701 CCGGGCGGCT CAACATGATC ATTGTCTATG ACGTGTCGCG CTTCTCGCGC CTGAAGGTCA
     2761 TGGACGCGAT TCCGATTGTC TCGGAATTGC TCGCCCTGGG CGTGACGATT GTTTCCACTC
     2821 AGGAAGGCGT CTTCCGGCAG GGAAACGTCA TGGACCTGAT TCACCTGATT ATGCGGCTCG
     2881 ACGCGTCGCA CAAAGAATCT TCGCTGAAGT CGGCGAAGAT TCTCGACACG AAGAACCTTC
     2941 AGCGCGAATT GGGCGGGTAC GTCGGCGGGA AGGCGCCTTA CGGCTTCGAG CTTGTTTCGG
     3001 AGACGAAGGA GATCACGCGC AACGGCCGAA TGGTCAATGT CGTCATCAAC AAGCTTGCGC
     3061 ACTCGACCAC TCCCCTTACC GGACCCTTCG AGTTCGAGCC CGACGTAATC CGGTGGTGGT
     3121 GGCGTGAGAT CAAGACGCAC AAACACCTTC CCTTCAAGCC GGGCAGTCAA GCCGCCATTC
     3181 ACCCGGGCAG CATCACGGGG CTTTGTAAGC GCATGGACGC TGACGCCGTG CCGACCCGGG
     3241 GCGAGACGAT TGGGAAGAAG ACCGCTTCAA GCGCCTGGGA CCCGGCAACC GTTATGCGAA
     3301 TCCTTCGGGA CCCGCGTATT GCGGGCTTCG CCGCTGAGGT GATCTACAAG AAGAAGCCGG
     3361 ACGGCACGCC GACCACGAAG ATTGAGGGTT ACCGCATTCA GCGCGACCCG ATCACGCTCC
     3421 GGCCGGTCGA GCTTGATTGC GGACCGATCA TCGAGCCCGC TGAGTGGTAT GAGCTTCAGG
     3481 CGTGGTTGGA CGGCAGGGGG CGCGGCAAGG GGCTTTCCCG GGGGCAAGCC ATTCTGTCCG
     3541 CCATGGACAA GCTGTACTGC GAGTGTGGCG CCGTCATGAC TTCGAAGCGC GGGGAAGAAT
     3601 CGATCAAGGA CTCTTACCGC TGCCGTCGCC GGAAGGTGGT CGACCCGTCC GCACCTGGGC
     3661 AGCACGAAGG CACGTGCAAC GTCAGCATGG CGGCACTCGA CAAGTTCGTT GCGGAACGCA
     3721 TCTTCAACAA GATCAGGCAC GCCGAAGGCG ACGAAGAGAC GTTGGCGCTT CTGTGGGAAG
     3781 CCGCCCGACG CTTCGGCAAG CTCACTGAGG CGCCTGAGAA GAGCGGCGAA CGGGCGAACC
     3841 TTGTTGCGGA GCGCGCCGAC GCCCTGAACG CCCTTGAAGA GCTGTACGAA GACCGCGCGG
     3901 CAGGCGCGTA CGACGGACCC GTTGGCAGGA AGCACTTCCG GAAGCAACAG GCAGCGCTGA
     3961 CGCTCCGGCA GCAAGGGGCG GAAGAGCGGC TTGCCGAACT TGAAGCCGCC GAAGCCCCGA
     4021 AGCTTCCCCT TGACCAATGG TTCCCCGAAG ACGCCGACGC TGACCCGACC GGCCCTAAGT
     4081 CGTGGTGGGG GCGCGCGTCA GTAGACGACA AGCGCGTGTT CGTCGGGCTC TTCGTAGACA
     4141 AGATCGTTGT CACGAAGTCG ACTACGGGCA GGGGGCAGGG AACGCCCATC GAGAAGCGCG
     4201 CTTCGATCAC GTGGGCGAAG CCGCCGACCG ACGACGACGA AGACGACGCC CAGGACGGCA
     4261 CGGAAGACGT AGCGGCGTAA tctatagtgt cacctaaatg ggtgccaggg cgtgcccGTg
     4321 ggctccccgg gcgcgtaTAG GGATAACAGG GTAATACACt GAGACCTCCA CATATACCTG
     4381 CCGTTCACTA TTATTTAGTG AAATGAGATA TTATGATATT TTCTGAATTG TGATTAAAAA
     4441 GGCAACTTTA TGCCCATGCA ACAGAAACTA TAAAAAATAC AGAGAATGAA AAGAAACAGA
     4501 TAGATTTTTT AGTTCTTTAG GCCCGTAGTC TGCAAATCCT TTTATGATTT TCTATCAAAC
     4561 AAAAGAGGAA AATAGACCAG TTGCAATCCA AACGAGAGTC TAATAGAATG AGGTCGAAAA
     4621 GTAAATCGCG CGGGTTTGTT ACTGATAAAG CAGGCAAGAC CTAAAATGTG TAAAGGGCAA
     4681 AGTGTATACT TTGGCGTCAC CCCTTACATA TTTTAGGTCT TTTTTTATTG TGCGTAACTA
     4741 ACTTGCCATC TTCAAACAGG AGGGCTGGAA GAAGCAGACC GCTAACACAG TACATAAAAA
     4801 AGGAGACATG AACGATGAAC ATCAAAAAGT TTGCAAAACA AGCAACAGTA TTAACCTTTA
     4861 CTACCGCACT GCTGGCAGGA GGCGCAACTC AAGCGTTTGC GAAAGAAACG AACCAAAAGC
     4921 CATATAAGGA AACATACGGC ATTTCaCATA TTACACGCCA TGATATGCTG CAAATCCCTG
     4981 AACAGCAAAA AAATGAAAAA TATCAAGTTC CTGAgTTCGA TTCGTCCACA ATTAAAAATA
     5041 TCTCTTCTGC AAAAGGCCTG GACGTTTGGG ACAGCTGGCC ATTACAAAAC GCTGACGGCA
     5101 CTGTCGCAAA CTATCACGGC TACCACATCG TCTTTGCATT AGCCGGAGAT CCTAAAAATG
     5161 CGGATGACAC ATCGATTTAC ATGTTCTATC AAAAAGTCGG CGAAACTTCT ATTGACAGCT
     5221 GGAAAAACGC TGGCCGCGTC TTTAAAGACA GCGACAAATT CGATGCAAAT GATTCTATCC
     5281 TAAAAGACCA AACACAAGAA TGGTCAGGTT CAGCCACATT TACATCTGAC GGAAAAATCC
     5341 GTTTATTCTA CACTGATTTC TCCGGTAAAC ATTACGGCAA ACAAACACTG ACAACTGCAC
     5401 AAGTTAACGT ATCAGCATCA GACAGCTCTT TGAACATCAA CGGTGTAGAG GATTATAAAT
     5461 CAATCTTTGA CGGTGACGGA AAAACGTATC AAAATGTACA GCAGTTCATC GATGAAGGCA
     5521 ACTACAGCTC AGGCGACAAC CATACGCTGA GAGATCCTCA CTACGTAGAA GATAAAGGCC
     5581 ACAAATACTT AGTATTTGAA GCAAACACTG GAACTGAAGA TGGCTACCAA GGCGAAGAAT
     5641 CTTTATTTAA CAAAGCATAC TATGGCAAAA GCACATCATT CTTCCGTCAA GAAAGTCAAA
     5701 AACTTCTGCA AAGCGATAAA AAACGCACGG CTGAGTTAGC AAACGGCGCT CTCGGTATGA
     5761 TTGAGCTAAA CGATGATTAC ACACTGAAAA AAGTGATGAA ACCGCTGATT GCATCTAACA
     5821 CAGTAACAGA TGAAATTGAA CGCGCGAACG TCTTTAAAAT GAACGGCAAA TGGTACCTGT
     5881 TCACTGACTC CCGCGGATCA AAAATGACGA TTGACGGCAT TACGTCTAAC GATATTTACA
     5941 TGCTTGGTTA TGTTTCTAAT TCTTTAACTG GCCCATACAA GCCGCTGAAC AAAACTGGCC
     6001 TTGTGTTAAA AATGGATCTT GATCCTAACG ATGTAACCTT TACTTACTCA CACTTCGCTG
     6061 TACCTCAAGC GAAAGGAAAC AATGTCGTGA TTACAAGCTA TATGACAAAC AGAGGATTCT
     6121 ACGCAGACAA ACAATCAACG TTTGCGCCAA GtTTCCTGCT GAACATCAAA GGCAAGAAAA
     6181 CATCTGTTGT CAAAGACAGC ATCCTTGAAC AAGGACAATT AACAGTTAAC AAATAAGGTC
     6241 TCtAGAGcca ccacagtggt taattaaGGC CtgtgaGGCC GGACCAAAAC GAAAAAAGGC
     6301 CCCCCTTTCG GGAGGCCTCT TTTCTGGAAT TTGGTACCGA Gacgtcaaat tctatcataa
     6361 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     6421 tgaaaaggct gttttctgta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     6481 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaAG AAATTGCATA
     6541 CCTTTGTTCC TCGGTTATAT GTTTGCTCAT CTGCAAgaca tggaggccca gaataccctc
     6601 cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag
     6661 cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga
     6721 agcaaaaatt acggcacctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac
     6781 gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca
     6841 ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc
     6901 acatccgaac ataaacaacc aaaaatttgt aattaagaag gagtgattac ATGGGTAAGG
     6961 AAAAGACTCA CGTTTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT
     7021 ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA
     7081 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA
     7141 CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC
     7201 ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGCAAAACAG
     7261 CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG
     7321 TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG
     7381 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GtGAGTGATT
     7441 TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAGCTTT
     7501 TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT
     7561 TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT
     7621 ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC
     7681 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA
     7741 TGCTCGATGA GTTTTTCTAA tcagtactga caataaaaag attcttgttt tcaagaactt
     7801 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     7861 ttatattttt tttcgcctcg acatcatctg tccagatgcg aagttaagtg cgcagaaagt
     7921 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctCTAGC ATAACCCCGC
     7981 GGGGCCTCTT TCGGGGATCT CGCGGGGTTT TTTGCTGAAA GAAGCTTCAA ATAAAACGAA
     8041 AGGCTCAGTC GAAAGACTGG GCCTTTCGTT ATGTTGTTGT CGCTGCGGCC GCACTCGAGC
     8101 ACCACCACCA CCACCACTGG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG
     8161 CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA
     8221 GGGGTTTTTT GCTGAAGTAC CGAGgggtgc cagggcgtgc ccCAgggctc cccgggcgcg
     8281 tataatcgat ttaaattagt agcccgccta atgagcgggc ttttttttaa ttcccctatt
     8341 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8401 atgcttcaat aatattgaaa aaggaagagt atgagcattc agcattttcg tgtggcgctg
     8461 attccgtttt ttgcggcgtt ttgcctgccg gtgtttgcgc atccggaaac cctggtgaaa
     8521 gtgaaagatg cggaagatca actgggtgcg cgcgtgggct atattgaact ggatctgaac
     8581 agcggcaaaa ttctggaatc ttttcgtccg gaagaacgtt ttccgatgat gagcaccttt
     8641 aaagtgctgc tgtgcggtgc ggttctgagc cgtgtggatg cgggccagga acaactgggc
     8701 cgtcgtattc attatagcca gaacgatctg gtggaatata gcccggtgac cgaaaaacat
     8761 ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg cgattaccat gagcgataac
     8821 accgcggcga acctgctgct gacgaccatt ggcggtccga aagaactgac cgcgtttctg
     8881 cataacatgg gcgatcatgt gacccgtctg gatcgttggg aaccggaact gaacgaagcg
     8941 attccgaacg atgaacgtga taccaccatg ccggcagcaa tggcgaccac cctgcgtaaa
     9001 ctgctgacgg gtgagctgct gaccctggca agccgccagc aactgattga ttggatggaa
     9061 gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc cggctggctg gtttattgcg
     9121 gataaaagcg gtgcgggcga acgtggcagc cgtggcatta ttgcggcgct gggcccggat
     9181 ggtaaaccga gccgtattgt ggtgatttat accaccggca gccaggcgac gatggatgaa
     9241 cgtaaccgtc agattgcgga aattggcgcg agcctgatta aacattggta aaccgataca
     9301 attaaaggct ccttttggag cctttttttt t
//