LOCUS       Exported               13117 bp ds-DNA     circular UNA 14-APR-2025
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local...5hz-j1qp7iz
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     rep_origin      53..641
                     /label=ori
     misc_feature    complement(827..967)
                     /label=bom
     rep_origin      complement(1311..1505)
                     /direction=LEFT
                     /label=pVS1 oriV
     CDS             complement(1571..2644)
                     /label=pVS1 RepA
     CDS             complement(3073..3702)
                     /label=pVS1 StaA
     primer_bind     complement(4200..4209)
                     /label=OLEO-RFP for seq4+
     primer_bind     4909..4928
                     /label=pLII for seq+
     primer_bind     4920..4941
                     /label=pKIR_Rev
     misc_feature    complement(5003..5027)
                     /label=RB T-DNA repeat
     misc_feature    5062..7055
                     /label=pLI - A-B proUBI10 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:6jq-f08w7nq"
     misc_feature    5062..5065
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        5066..7051
                     /label=UBI10 Promoter
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     primer_bind     5575..5597
                     /label=proUBI10 for seq2+
     primer_bind     complement(5924..5946)
                     /label=proUBI10 for seq2-
     primer_bind     complement(6278..6300)
                     /label=proUBI10 for seq3-
     primer_bind     6366..6388
                     /label=proUBI10 for seq3+
     primer_bind     complement(6729..6749)
                     /label=proUBI10 for seq-
     primer_bind     6793..6813
                     /label=proUBI10 for seq+
     misc_feature    7052..8064
                     /label=pLI - B-C CKA3 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:6jo-f08w7nq"
     misc_feature    7052..8064
                     /label=B-C CKA3 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:97o-f08w7wm"
     misc_feature    7052..7059
                     /label=GG B-C fwd Kozak
                     /note="/Color=-1"
     misc_feature    7052..7055
                     /label=Ligation
                     /label=nonstandard type:  ligation
     CDS             7060..8058
                     /label=CKA3
                     /note="/created_by=Chico Sababa"
     primer_bind     7060..7082
                     /label=CKA3 B-C fwd
     primer_bind     7126..7146
                     /label=CKA3 for seq1+
     primer_bind     complement(7193..7214)
                     /label=CKA3 for seq1-
     misc_feature    7246..7248
                     /label=K63M (Kinase dead)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     misc_feature    7247
                     /label=A
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=A"
     misc_feature    7264..7266
                     /label=K69A (InsP binding)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     misc_feature    7270..7272
                     /label=K71A (InsP binding)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
     misc_feature    7273..7275
                     /label=K72A (InsP binding)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
     misc_feature    7567..7569
                     /label=D170N (Kinase dead)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     misc_feature    7567
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    7569
                     /label=T
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=T"
     primer_bind     7632..7651
                     /label=CKA3 for seq2+
     misc_feature    7783..7785
                     /label=K242A (InsP binding)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     misc_feature    7870..7872
                     /label=H271A (InsP binding)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
     misc_feature    7879..7881
                     /label=K274A (InsP binding)
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     primer_bind     complement(7899..7918)
                     /label=CKA3 for seq2-
     primer_bind     complement(8036..8058)
                     /label=CKA3 B-C rev
     misc_feature    8056
                     /label=A
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=A"
     misc_feature    complement(8059..8064)
                     /label=GG B-C rev Gly
                     /note="/Color=-1"
     misc_feature    8061..8098
                     /label=pLI - C-D Linker (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:6jp-f08w7nq"
     misc_feature    8061..8098
                     /label=Linker (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:bsb-f08w8a0"
     misc_feature    8061..8064
                     /label=Ligation
                     /label=nonstandard type:  ligation
     CDS             8065..8094
                     /label=Linker CDS
                     /note="/created_by=Chico Sababa"
     misc_feature    8095..8173
                     /label=pLI - D-E FLAG assembly (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:6jt-f08w7nq"
     misc_feature    8095..8173
                     /label=FLAG for DE (digested by BbsI) Fragment 2
                     (conc
                     /label=FLAG for DE (digested by BbsI) Fragment 2
                     (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:brq-f08w89y"
     misc_feature    8095..8098
                     /label=Ligation
                     /label=nonstandard type:  ligation
     CDS             8101..8166
                     /label=3XFLAG
                     /note="/created_by=Chico Sababa"
     misc_feature    8170..8430
                     /label=pLI - E-F termNOS (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:6jk-f08w7np"
     misc_feature    8170..8173
                     /label=Ligation
                     /label=nonstandard type:  ligation
     terminator      8174..8426
                     /label=NOS terminator
     primer_bind     complement(8250..8274)
                     /label=OP746-termNOS seq-
     primer_bind     8313..8336
                     /label=OP747-termNOS seq+
     misc_feature    8427..11517
                     /label=pLI - F G OLE1-RFP correct (concatenated sequen
                     /label=pLI - F G OLE1-RFP correct (concatenated sequence
                     7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:6jj-f08w7np"
     misc_feature    8427..8430
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        8431..9740
                     /label=OLEO1 Promoter
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Promoter Olesin (A. thaliana) (pDGE146_PcUbi4-2_Cas9_)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Promoter
                     Olesin (A. thaliana) (pDGE146_test)"
                     /note="/Transferred_From=pDGE146_test"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=Chico Sababa"
     primer_bind     8492..8514
                     /label=OLEO-RFP for seq1+
     primer_bind     9181..9203
                     /label=OLEO-RFP for seq2+
     primer_bind     complement(9246..9268)
                     /label=OLEO-RFP for seq1-
     misc_feature    9493
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     5'UTR           9741..9808
                     /label=5'UTR
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     5'UTR Olesin (A. thaliana) (pDGE146_PcUbi4-2_Cas9_)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     5'UTR
                     Olesin (A. thaliana) (pDGE146_test)"
                     /note="/Transferred_From=pDGE146_test"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=Chico Sababa"
     CDS             join(9809..10161,10402..10567)
                     /label=OLEO1
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     primer_bind     9913..9935
                     /label=OLEO-RFP for seq3+
     primer_bind     complement(10013..10035)
                     /label=OLEO-RFP for seq2-
     primer_bind     10559..10581
                     /label=OLEO-RFP for seq4+
     CDS             10568..11263
                     /label=RFP
                     /note="/created_by=Chico Sababa"
     primer_bind     complement(10803..10825)
                     /label=OLEO-RFP for seq3-
     misc_feature    10864
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    10867
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    10882
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    10990
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    11011
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    11014
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    11023
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    11026
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     primer_bind     11111..11133
                     /label=OLEO-RFP for seq5+
     terminator      11264..11513
                     /label=AT5G59690 Terminator
                     /note="/created_by=Chico Sababa"
     misc_feature    join(11514..13117,1..5065)
                     /label=pLII beta R 5-6 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:6jh-f08w7np"
     misc_feature    11514..11517
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    complement(11554..11578)
                     /label=LB T-DNA repeat
     primer_bind     complement(11606..11625)
                     /label=pLII for seq-
     CDS             complement(11956..12747)
                     /label=SmR
ORIGIN
        1 tgccgttctt ccgatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
       61 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      121 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      181 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      421 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      601 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      661 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      721 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      781 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
      841 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
      901 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
      961 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1021 ggcatccgct tacagacaag ctgtgactgt ctccgggagc tgcatgtgtc agaggttttc
     1081 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     1141 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     1201 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     1261 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     1321 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     1381 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     1441 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     1501 ccacaggaaa gagtcctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     1561 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     1621 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     1681 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     1741 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     1801 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     1861 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     1921 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     1981 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     2041 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     2101 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
     2161 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
     2221 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
     2281 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
     2341 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
     2401 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
     2461 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
     2521 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
     2581 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
     2641 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca
     2701 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     2761 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     2821 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
     2881 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
     2941 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
     3001 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
     3061 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
     3121 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
     3181 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
     3241 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
     3301 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
     3361 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
     3421 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
     3481 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
     3541 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
     3601 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
     3661 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
     3721 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
     3781 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
     3841 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
     3901 atgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
     3961 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
     4021 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
     4081 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
     4141 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
     4201 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
     4261 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
     4321 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
     4381 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
     4441 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
     4501 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
     4561 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
     4621 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
     4681 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
     4741 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
     4801 tgtgcgacat gtcgttttca gaagaaggct gcactgaacg tcagaagccg actgcactat
     4861 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
     4921 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
     4981 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
     5041 taaaccagag aagacaacag agcggagtct agctcaacag agcttttaac ccaaattggt
     5101 acaatagaat acaactttag atcataattc tcaaaagaaa gagattcctt agctattcta
     5161 tctgccactc catttccttc tcggcttgta tgcacaagca taaaatcctc aaacttgcta
     5221 agtagatact ttatgtcttg gataattgga ttgagacttg acaagcataa ctttcatgta
     5281 accaaagaca caagttgctg agaatccacc tcaaaaatga tcttcctata attgaatcgg
     5341 gataatgaca gcacagccca tctaagagcc tccacttcta cttccagcac gcttcttact
     5401 tttaccacag ctcttgcacc taaccataac accttccctg tatgatcgcg aagcacccac
     5461 cctaagccac attttaatcc ttctgttggc catgccccat caaagttgca cttaacccaa
     5521 gattgtggtg gagcttccca tgtttctcgt ctgtcccgac ggtgttgtgg ttggtgcttt
     5581 ccttacattc tgagcctctt tccttctaat ccactcatct gcatcttctt gtgtccttac
     5641 taatacctca ttggttccaa attccctccc tttaagcacc agctcgtttc tgttcttcca
     5701 cagcctccca agtatccaag ggactaaagc ctccacattc ttcagatcag gatattcttg
     5761 tttaagatgt tgaactctat ggaggtttgt atgaactgat gatctaggac cggataagtt
     5821 cccttcttca tagcgaactt attcaaagaa tgttttgtgt atcattcttg ttacattgtt
     5881 attaatgaaa aaatattatt ggtcattgga ctgaacacga gtgttaaata tggaccaggc
     5941 cccaaataag atccattgat atatgaatta aataacaaga ataaatcgag tcaccaaacc
     6001 acttgccttt tttaacgaga cttgttcacc aacttgatac aaaagtcatt atcctatgca
     6061 aatcaataat catacaaaaa tatccaataa cactaaaaaa ttaaaagaaa tggataattt
     6121 cacaatatgt tatacgataa agaagttact tttccaagaa attcactgat tttataagcc
     6181 cacttgcatt agataaatgg caaaaaaaaa caaaaaggaa aagaaataaa gcacgaagaa
     6241 ttctagaaaa tacgaaatac gcttcaatgc agtgggaccc acggttcaat tattgccaat
     6301 tttcagctcc accgtatatt taaaaaataa aacgataatg ctaaaaaaat ataaatcgta
     6361 acgatcgtta aatctcaacg gctggatctt atgacgaccg ttagaaattg tggttgtcga
     6421 cgagtcagta ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc
     6481 aactcaaagc acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact
     6541 ttgcgtgtaa acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc
     6601 ttagctttct cgtgacctag tcgtcctcgt cttttcttct tcttcttcta taaaacaata
     6661 cccaaagagc tcttcttctt cacaattcag atttcaattt ctcaaaatct taaaaacttt
     6721 ctctcaattc tctctaccgt gatcaaggta aatttctgtg ttccttattc tctcaaaatc
     6781 ttcgattttg ttttcgttcg atcccaattt cgtatatgtt ctttggttta gattctgtta
     6841 atcttagatc gaatacgatt ttctgggttt gatcgttaga tatcatctta attctcgatt
     6901 agggtttcat agatatcatc cgatttgttc aaataatttg agttttgtcg aataattact
     6961 cttcgatttg tgatttctat ctagatctgg tgttagtttc tagtttgtgc gatcgaattt
     7021 gtcgattaat ctgagttttt ctgattaaca gtctgaacaa tgtcgaaagc tagggtttat
     7081 acagatgtta acgttgttcg tcctaaagag tattgggact acgagtctct cgttgttcaa
     7141 tggggtcatc aagatgatta tgaggttgtg agaaaagttg gtagaggaaa atacagtgaa
     7201 gttttcgaag gcaaaaacgt caatacgaat gagcgttgcg ttatcatgat cctcaaaccc
     7261 gtaaaaaaga agaagataaa gagagagatc aaaatacttc agaacctatg tggtggacca
     7321 aatattgtta agctgtatga tattgttaga gatgagcact ccaaaacacc tagcttggta
     7381 tttgagtttg tcaacagtgt ggactttaaa gtattgtatc caacattgac tgattacgat
     7441 atccggtact atatttatga gcttctgaag gcattggatt tctgtcactc tcaaggaata
     7501 atgcataggg atgttaagcc tcataatgtc atgatagatc atcagctgcg taaacttcgt
     7561 ctgataaact ggggccttgc tgagttttac catcccggaa aagagtataa tgttcgcgtg
     7621 gcctcgagat acttcaaggg tcccgagctt ttagttgatc tacaggatta tgactattct
     7681 ttagacatgt ggagtcttgg ttgcatgttt gctggcatga tattccgtaa ggaaccgttc
     7741 ttttatggcc atgataatca tgatcaactt gtcaaaattg ccaaggtgtt gggaaccaat
     7801 gagttagatc attacctaaa caaatatcag ctagatcttg accctcaact ggaagcactt
     7861 gttggaaggc atgtccccaa accatggtcc aagtttatca acgcggacaa ccaacatttg
     7921 gtgtcacctg aggcaattga ttttctcgat aagcttcttc agtatgatca tcaagacaga
     7981 ttaaccgcaa gagaagccat ggatcatcca tacttcgctc aagttaaagc cgcagagagc
     8041 agcagactac gaactcaggg caccggtggt tcgggttctg gaggaggtgg atctaaggga
     8101 gattacaaag accatgatgg agattataag gatcacgata tcgactacaa agatgacgac
     8161 gataaataaa atcgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     8221 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     8281 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     8341 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     8401 cgcggtgtca tctatgttac tagatctgag gtatgtaggt atagtaacat gaacaagaac
     8461 agactcaagt acaagatcgc atacgaaaat ggaaatggca atgtcacttc cacataatca
     8521 aacacgaatc ctcatatcaa caaggcctga gattctaact agctcataac aacttagcca
     8581 atagttactt gagactacca aatgtatgta gaactaaaga ctaagggaca gagagttcgt
     8641 ctaaacaggt gaatctagtc gttgttatct aataaacaat tcagccccaa atgcagaaca
     8701 cacatagagc tctctattga ttcaaattac gatctgatac tgataacgtc tagattttta
     8761 gggttaaagc aatcaatcac ctgacgattc aaggtggttg gatcatgacg attccagaaa
     8821 acatcaagca agctctcaaa gctacactct ttgggatcat actgaactct aacaacctcg
     8881 ttatgtcccg tagtgccagt acagacatcc tcgtaactcg gattgtgcac gatgccatgg
     8941 ctatacccaa cctcggtctt ggtcacacca ggaactctct ggtaagctag ctccactccc
     9001 cagaaacaac cggcgccaaa ttgcgcgaat tgctgacctg atgacggaac atcatcgtcg
     9061 ggtccttggg cgattgcggc ggaagatggg tcagcttggg cttgaggacg agagccgaat
     9121 ccgagtctgt tgaaaaggtt gttcattggg gatttgtata cggagattgg tcgtcgagag
     9181 gtttgaggga aaggacaaat gggtttggct ctggagaaag agagtgcggc tttagagaga
     9241 gaattgagag gtttagagag agatgcggcg gcgatgagcg tgccgaggag agacgacgag
     9301 gacctgcatt atcaaagcag tgacgtggtg aaatttggaa cttttaagag gcagatagat
     9361 ttattatttg tatccatttt cttcattgtt ctagaatgtc gcggaacaaa ttttaaaact
     9421 aaatcctaaa tttttctaat tttgttgcca atagtggata tgtgggccgt atagaaggaa
     9481 tctattgaag gctcaaaccc atactgacga gcccaaaggt tcgttttgcg ttttatgttt
     9541 cggttcgatg ccaacgccac attctgagct aggcaaaaaa caaacgtgtc tttgaataga
     9601 ctcctctcgt taacacatgc agcggctgca tggtgacgcc attaacacgt ggcctacaat
     9661 tgcatgatgt ctccattgac acgtgacttc tcgtctcctt tcttaatata tctaacaaac
     9721 actcctacct cttccaaaat atatacacat ctttttgatc aatctctcat tcaaaatctc
     9781 attctctcta gtaaacaaga acaaaaaaat ggcggataca gctagaggaa cccatcacga
     9841 tatcatcggc agggaccagt acccgatgat gggccgtgac cgtgaccagt accagatgtc
     9901 cggacgagga tctgactact ccaagtctag gcagattgct aaagctgcaa ctgctgtcac
     9961 agctggtggt tccctccttg ttctctccag ccttaccctt gttggaactg tcatagcttt
    10021 gactgttgca acacctctgc tcgttatctt cagcccaatc cttgtcccgg ctctcatcac
    10081 agttgcactc ctcatcaccg gttttctttc ctctggaggg tttggcattg ccgctataac
    10141 cgttttctct tggatttaca agtaagcaca catttatcat cttacttcat aattttgtgc
    10201 aatatgtgca tgcatgtgtt gagccagtag ctttggatca atttttttgg tcgaataaca
    10261 aatgtaacaa taagaaattg caaattctag ggaacatttg gttaactaaa tacgaaattt
    10321 gacctagcta gcttgaatgt gtctgtgtat atcatctata taggtaaaat gcttggtatg
    10381 atacctattg attgtgaata ggtacgcaac gggagagcac ccacagggat cagacaagtt
    10441 ggacagtgca aggatgaagt tgggaagcaa agctcaggat ctgaaagaca gagctcagta
    10501 ctacggacag caacatactg gtggggaaca tgaccgtgac cgtactcgtg gtggccagca
    10561 cactactatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
    10621 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
    10681 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
    10741 ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg gcatccccga
    10801 cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgagga
    10861 cggaggtgtg ctgaccgcta ctcaggacac cagcctccag gacggctgcc tcatctacaa
    10921 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
    10981 cggctgggaa gccaacaccg agatgctgta tccagctgac ggaggactgg aaggcagaag
    11041 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca agaccacata
    11101 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggaccacag
    11161 actggaaaga atcaaggagg ccgacaaaga aacctacgtc gagcagcacg aggtggctgt
    11221 ggccagatac tgcgacctcc ctagcaaact ggggcacaag tgattagagt ttttcagatc
    11281 cgcgtttgtg ttttctgggt ttctcactta agcgtctgcg ttttactttt gtattgggtt
    11341 tggcgtttag tagtttgcgg tagcgttctt gttatgtgta attacgcttt ttcttcttgc
    11401 ttcagcagtt tcggttgaaa tataaatcga atcaagtttc actttatcag cgttgtttta
    11461 aattttggca ttaaattggt gaaaattgct tcaattttgt atctaaatag aagtgtcgac
    11521 attgtcttcc gtaatccgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
    11581 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
    11641 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
    11701 gttaccggca gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11761 ctcacgttaa gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta
    11821 tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg
    11881 tgcttagtgc atctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga
    11941 atttctagct agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
    12001 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
    12061 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
    12121 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
    12181 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
    12241 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
    12301 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
    12361 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
    12421 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
    12481 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
    12541 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
    12601 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
    12661 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
    12721 tacttcggcg atcaccgctt cccccatgat gtttaacttt gttttagggc gactgccctg
    12781 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
    12841 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa
    12901 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
    12961 gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc actgggttcg
    13021 tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
    13081 cgcgagatca tccgtgtttc aaacccggca gcttagt
//
