LOCUS       Exported               18365 bp ds-DNA     circular UNA 20-SEP-2022
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local...fhu-fbsyunb
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 18365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..3059,16435..18365)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          join(3056..3063,5022..5029)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          join(5026..5033,6992..6999)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          6996..7032
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          7029..7065
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          join(7062..7069,16431..16438)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     rep_origin      316..510
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(576..1649)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2078..2707)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     primer_bind     2924..2943
                     /label=pLII for seq-
     misc_feature    2971..2995
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    3056..5029
                     /label=LIIalpha F 1-2 CKB1 and CKB2 guides (concatenat
                     /label=LIIalpha F 1-2 CKB1 and CKB2 guides (concatenated 
                     sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fgd-fbsyqr7"
     misc_feature    3056..3059
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    3060..3390
                     /label=pLI-sgRNA_AB - CKB1 Guide 1 (concatenated seque
                     /label=pLI-sgRNA_AB - CKB1 Guide 1 (concatenated sequence
                     2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fcg-fbsxuma"
     misc_feature    3060..3063
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        3067..3271
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    3269..3295
                     /label=CKB1 Guide 1 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9jn-f6otcu9"
     misc_feature    3269..3272
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    3269..3272
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    3272
                     /label=initial G for transcription
     unsure          3273..3291
                     /label=CKB1 Guide 1
                     /note="/created_by=oprital"
     misc_feature    join(3292..3390,3060..3272)
                     /label=pLI-sgRNA_AB (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9nt-f6otm1u"
     misc_feature    3292..3367
                     /label=sgRNA_scaffold
     misc_feature    3292..3295
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    3292..3295
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      3368..3374
                     /label=regulatory
     misc_feature    3387..3717
                     /label=pLI-sgRNA_BC - CKB1 Guide 2 (concatenated seque
                     /label=pLI-sgRNA_BC - CKB1 Guide 2 (concatenated sequence
                     3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fch-fbsxuma"
     misc_feature    3387..3390
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        3394..3598
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    3596..3622
                     /label=CKB1 Guide 2 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9jl-f6otcu9"
     misc_feature    3596..3599
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    3596..3599
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    3599
                     /label=initial G for transcription
     unsure          3600..3618
                     /label=CKB1 Guide 2
                     /note="/created_by=oprital"
     misc_feature    join(3619..3717,3387..3599)
                     /label=pLI-sgRNA_BC (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9nr-f6otm1u"
     misc_feature    3619..3694
                     /label=sgRNA_scaffold
     misc_feature    3619..3622
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    3619..3622
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      3695..3701
                     /label=regulatory
     misc_feature    3714..4044
                     /label=pLI-sgRNA_CD - CKB1 Guide 3 (concatenated seque
                     /label=pLI-sgRNA_CD - CKB1 Guide 3 (concatenated sequence
                     4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fci-fbsxuma"
     misc_feature    3714..3717
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        3721..3925
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    3923..3949
                     /label=CKB1 Guide 3 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9jm-f6otcu9"
     misc_feature    3923..3926
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    3923..3926
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    3926
                     /label=initial G for transcription
     unsure          3927..3945
                     /label=CKB1 Guide 3
                     /note="/created_by=oprital"
     misc_feature    join(3946..4044,3714..3926)
                     /label=pLI-sgRNA_CD (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9ns-f6otm1u"
     misc_feature    3946..4021
                     /label=sgRNA_scaffold
     misc_feature    3946..3949
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    3946..3949
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      4022..4028
                     /label=regulatory
     misc_feature    4041..4371
                     /label=pLI-sgRNA_DE - CKB2 Guide 1 (concatenated seque
                     /label=pLI-sgRNA_DE - CKB2 Guide 1 (concatenated sequence
                     5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fd0-fbsxv4n"
     misc_feature    4041..4044
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        4048..4252
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    4250..4276
                     /label=CKB2 Guide 1 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9k1-f6otd4p"
     misc_feature    4250..4253
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    4250..4253
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    4253
                     /label=initial G for transcription
     unsure          4254..4272
                     /label=CKB2 Guide 1
                     /note="/created_by=oprital"
                     /note="/modified_by=oprital"
     misc_feature    join(4273..4371,4041..4253)
                     /label=pLI-sgRNA_DE (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9oi-f6otmhk"
     misc_feature    4273..4348
                     /label=sgRNA_scaffold
     misc_feature    4273..4276
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    4273..4276
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      4349..4355
                     /label=regulatory
     misc_feature    4368..4698
                     /label=pLI-sgRNA_EF - CKB2 Guide 2 (concatenated seque
                     /label=pLI-sgRNA_EF - CKB2 Guide 2 (concatenated sequence
                     6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fd1-fbsxv4n"
     misc_feature    4368..4371
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        4375..4579
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    4577..4603
                     /label=CKB2 Guide 2 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9jz-f6otd4p"
     misc_feature    4577..4580
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    4577..4580
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    4580
                     /label=initial G for transcription
     unsure          4581..4599
                     /label=CKB2 Guide 2
                     /note="/created_by=oprital"
     misc_feature    join(4600..4698,4368..4580)
                     /label=pLI-sgRNA_EF (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9oj-f6otmhk"
     misc_feature    4600..4675
                     /label=sgRNA_scaffold
     misc_feature    4600..4603
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    4600..4603
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      4676..4682
                     /label=regulatory
     misc_feature    4695..5025
                     /label=pLI-sgRNA_FG - CKB2 Guide 3 (concatenated seque
                     /label=pLI-sgRNA_FG - CKB2 Guide 3 (concatenated sequence
                     7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fd2-fbsxv4n"
     misc_feature    4695..4698
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        4702..4906
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    4904..4930
                     /label=CKB2 Guide 3 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9k0-f6otd4p"
     misc_feature    4904..4907
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    4904..4907
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    4907
                     /label=initial G for transcription
     unsure          4908..4926
                     /label=CKB2 Guide 3
                     /note="/created_by=oprital"
     misc_feature    join(4927..5025,4695..4907)
                     /label=pLI-sgRNA_FG (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9ol-f6otmhk"
     misc_feature    4927..5002
                     /label=sgRNA_scaffold
     misc_feature    4927..4930
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    4927..4930
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      5003..5009
                     /label=regulatory
     misc_feature    join(5022..5029,3056..3063)
                     /label=LIIalpha F 1-2 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:ffg-fbsy3mo"
     misc_feature    5022..5025
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    5026..6999
                     /label=LIIalpha F 2-3 CKB3 and CKB4 guides (concatenat
                     /label=LIIalpha F 2-3 CKB3 and CKB4 guides (concatenated 
                     sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fgl-fbsyr7u"
     misc_feature    5026..5029
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    5030..5360
                     /label=pLI-sgRNA_AB - CKB3 Guide 1 (concatenated seque
                     /label=pLI-sgRNA_AB - CKB3 Guide 1 (concatenated sequence
                     2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fdk-fbsxviy"
     misc_feature    5030..5033
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        5037..5241
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    5239..5265
                     /label=CKB3 Guide 1 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:4or-f34wweh"
     misc_feature    5239..5242
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    5239..5242
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    5242
                     /label=initial G for transcription
     unsure          5243..5261
                     /label=CKB3 Guide 1
                     /note="/created_by=oprital"
                     /note="/modified_by=oprital"
     misc_feature    join(5262..5360,5030..5242)
                     /label=pLI-sgRNA_AB (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9o7-f6otm6z"
     misc_feature    5262..5337
                     /label=sgRNA_scaffold
     misc_feature    5262..5265
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    5262..5265
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      5338..5344
                     /label=regulatory
     misc_feature    5357..5687
                     /label=pLI-sgRNA_BC - CKB3 Guide 2 (concatenated seque
                     /label=pLI-sgRNA_BC - CKB3 Guide 2 (concatenated sequence
                     3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fdl-fbsxviy"
     misc_feature    5357..5360
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        5364..5568
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    5566..5592
                     /label=CKB3 Guide 2 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:4oo-f34wwef"
     misc_feature    5566..5569
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    5566..5569
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    5569
                     /label=initial G for transcription
     unsure          5570..5588
                     /label=CKB3 Guide 2
                     /note="/created_by=oprital"
                     /note="/modified_by=oprital"
     misc_feature    join(5589..5687,5357..5569)
                     /label=pLI-sgRNA_BC (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9o5-f6otm6z"
     misc_feature    5589..5664
                     /label=sgRNA_scaffold
     misc_feature    5589..5592
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    5589..5592
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      5665..5671
                     /label=regulatory
     misc_feature    5684..6014
                     /label=pLI-sgRNA_CD - CKB3 Guide 3 (concatenated seque
                     /label=pLI-sgRNA_CD - CKB3 Guide 3 (concatenated sequence
                     4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fdm-fbsxviy"
     misc_feature    5684..5687
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        5691..5895
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    5893..5919
                     /label=CKB3 Guide 3 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:4ou-f34wweh"
     misc_feature    5893..5896
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    5893..5896
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    5896
                     /label=initial G for transcription
     unsure          5897..5915
                     /label=CKB3 Guide 3
                     /note="/created_by=oprital"
                     /note="/modified_by=oprital"
     misc_feature    join(5916..6014,5684..5896)
                     /label=pLI-sgRNA_CD (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9o6-f6otm6z"
     misc_feature    5916..5991
                     /label=sgRNA_scaffold
     misc_feature    5916..5919
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    5916..5919
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      5992..5998
                     /label=regulatory
     misc_feature    6011..6341
                     /label=pLI-sgRNA_DE - CKB4 Guide 1 (concatenated seque
                     /label=pLI-sgRNA_DE - CKB4 Guide 1 (concatenated sequence
                     5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fe4-fbsxvv8"
     misc_feature    6011..6014
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        6018..6222
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    6220..6246
                     /label=CKB4 Guide 1 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9kt-f6ote95"
     misc_feature    6220..6223
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    6220..6223
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    6223
                     /label=initial G for transcription
     unsure          6224..6242
                     /label=CKB4 Guide 1
                     /note="/created_by=oprital"
     misc_feature    join(6243..6341,6011..6223)
                     /label=pLI-sgRNA_DE (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9ow-f6otml6"
     misc_feature    6243..6318
                     /label=sgRNA_scaffold
     misc_feature    6243..6246
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    6243..6246
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      6319..6325
                     /label=regulatory
     misc_feature    6338..6668
                     /label=pLI-sgRNA_EF - CKB4 Guide 2 (concatenated seque
                     /label=pLI-sgRNA_EF - CKB4 Guide 2 (concatenated sequence
                     6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fe5-fbsxvv8"
     misc_feature    6338..6341
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        6345..6549
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    6547..6573
                     /label=CKB4 Guide 2 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9kr-f6ote95"
     misc_feature    6547..6550
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    6547..6550
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    6550
                     /label=initial G for transcription
     unsure          6551..6569
                     /label=CKB4 Guide 2
                     /note="/created_by=oprital"
     misc_feature    join(6570..6668,6338..6550)
                     /label=pLI-sgRNA_EF (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9ox-f6otml7"
     misc_feature    6570..6645
                     /label=sgRNA_scaffold
     misc_feature    6570..6573
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    6570..6573
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      6646..6652
                     /label=regulatory
     misc_feature    6665..6995
                     /label=pLI-sgRNA_FG - CKB4 Guide 3 (concatenated seque
                     /label=pLI-sgRNA_FG - CKB4 Guide 3 (concatenated sequence
                     7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fe6-fbsxvv8"
     misc_feature    6665..6668
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        6672..6876
                     /label=AtU6.26 promoter
                     /note="/modified_by=oprital"
     misc_feature    6874..6900
                     /label=CKB4 Guide 3 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9ks-f6ote95"
     misc_feature    6874..6877
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    6874..6877
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    6877
                     /label=initial G for transcription
     unsure          6878..6896
                     /label=CKB4 Guide 3
                     /note="/created_by=oprital"
     misc_feature    join(6897..6995,6665..6877)
                     /label=pLI-sgRNA_FG (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:9oz-f6otml7"
     misc_feature    6897..6972
                     /label=sgRNA_scaffold
     misc_feature    6897..6900
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    6897..6900
                     /label=Ligation
                     /label=nonstandard type:  ligation
     regulatory      6973..6979
                     /label=regulatory
     misc_feature    join(6992..6999,5026..5033)
                     /label=LIIalpha F 2-3 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:ffl-fbsy3sf"
     misc_feature    6992..6995
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    6996..7032
                     /label=LII dy 3-4 (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fh0-fbsysgu"
     misc_feature    6996..6999
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    7029..7065
                     /label=LII dy 4-5 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fh1-fbsysgu"
     misc_feature    7029..7032
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    7062..16438
                     /label=LIIbeta F 5-6-Cas9 and OLEO-RFP (concatenated
                     s
                     /label=LIIbeta F 5-6-Cas9 and OLEO-RFP (concatenated
                     sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fgt-fbsyrj6"
     misc_feature    7062..7065
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    7066..13269
                     /label=pLI-AB-Cas9 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:b8m-f73z380"
     misc_feature    7066..7069
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        7070..8734
                     /label=AtRPS5a Promoter
                     /note="/modified_by=Chico Sababa"
     primer_bind     complement(7195..7215)
                     /label=Cas9 for seq-
     CDS             8735..8803
                     /label=3xFLAG
                     /note="/modified_by=Chico Sababa"
     CDS             8804..8854
                     /label=NLS
                     /note="/modified_by=Chico Sababa"
     CDS             8855..12955
                     /label=human-codon-optimized SpCas9
                     /note="/modified_by=Chico Sababa"
     CDS             12956..13006
                     /label=nucleoplasmin NLS peptide
                     /note="/modified_by=Chico Sababa"
     terminator      13016..13265
                     /label=Heat shock protein 18.2 terminator
                     /note="/modified_by=Chico Sababa"
     primer_bind     13189..13211
                     /label=Cas9 for seq+
     primer_bind     13218..13242
                     /label=DC819_Cas9_seqF
     misc_feature    13266..13277
                     /label=LI dy B-C (Plasmid #54342) (concatenated sequen
                     /label=LI dy B-C (Plasmid #54342) (concatenated sequence
                     3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:b7x-f73ywv8"
     CDS             complement(13266..13277)
                     /codon_start=2
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="VLP"
     misc_feature    13266..13269
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    13270..13273
                     /label=B-C dy
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     misc_feature    13274..13299
                     /label=LI dy C-D (Plasmid #54343) (concatenated sequen
                     /label=LI dy C-D (Plasmid #54343) (concatenated sequence
                     4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:b7y-f73ywv9"
     misc_feature    13274..13277
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    13278..13295
                     /label=C-D dy
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     misc_feature    13296..13325
                     /label=LI dy D-E (Plasmid #54344) (concatenated sequen
                     /label=LI dy D-E (Plasmid #54344) (concatenated sequence
                     5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:b7z-f73ywv9"
     misc_feature    13296..13299
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    13300..13321
                     /label=D-E dy
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     misc_feature    13322..13347
                     /label=pLI - E-F Dummy  (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:b8c-f73yzuh"
     misc_feature    13322..13325
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    13344..16434
                     /label=pLI-FG-OLE1-RFP correct (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:b8n-f73z380"
     misc_feature    13344..13347
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        13348..14657
                     /label=OLEO1 Promoter
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     Promoter Olesin (A. thaliana) (pDGE146_PcUbi4-2_Cas9_)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Promoter
                     Olesin (A. thaliana) (pDGE146_test)"
                     /note="/Transferred_From=pDGE146_test"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=Chico Sababa"
     primer_bind     13409..13431
                     /label=OLEO-RFP for seq1+
     primer_bind     complement(13446..13468)
                     /label=OLEO-RFP for seq4-
     primer_bind     14098..14120
                     /label=OLEO-RFP for seq2+
     primer_bind     complement(14163..14185)
                     /label=OLEO-RFP for seq1-
     misc_feature    14410
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     5'UTR           14658..14725
                     /label=5'UTR
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     5'UTR Olesin (A. thaliana) (pDGE146_PcUbi4-2_Cas9_)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     5'UTR
                     Olesin (A. thaliana) (pDGE146_test)"
                     /note="/Transferred_From=pDGE146_test"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=Chico Sababa"
     CDS             join(14726..15078,15319..15484)
                     /label=OLEO1
                     /note="/created_by=Chico Sababa"
                     /note="/modified_by=Chico Sababa"
     primer_bind     14830..14852
                     /label=OLEO-RFP for seq3+
     primer_bind     complement(14930..14952)
                     /label=OLEO-RFP for seq2-
     primer_bind     15476..15498
                     /label=OLEO-RFP for seq4+
     CDS             15485..16180
                     /label=RFP
                     /note="/created_by=Chico Sababa"
     primer_bind     complement(15720..15742)
                     /label=OLEO-RFP for seq3-
     misc_feature    15781
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    15784
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    15799
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    15907
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    15928
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    15931
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    15940
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    15943
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     primer_bind     16028..16050
                     /label=OLEO-RFP for seq5+
     terminator      16181..16430
                     /label=AT5G59690 Terminator
                     /note="/created_by=Chico Sababa"
     misc_feature    join(16431..16438,7062..7069)
                     /label=LIIbeta F 5-6 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:b8x-f73z3nw"
     misc_feature    16431..16434
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    join(16435..18365,1..3059)
                     /label=LIIIalpha fin (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument: 
                     urn:local:.:fh2-fbsysgu"
     misc_feature    16435..16438
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    16457..16481
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     complement(16563..16582)
                     /label=pLIIalpha for seq-
     rep_origin      complement(16604..17192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16684..16703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(17330..17349)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(17331..17349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(17354..17370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17354..17370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(17472..17491)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             17483..18292
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(17550..17569)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc tgccattttt
       61 ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag ctcggtgcct
      121 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
      181 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
      241 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
      301 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
      361 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
      421 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
      481 tttgggttcc caatgtacgt gctatccaca ggaaagaagc tttttcgacc tttttcccct
      541 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
      601 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
      661 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
      721 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
      781 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
      841 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
      901 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
      961 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
     1021 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
     1081 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
     1141 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
     1201 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
     1261 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
     1321 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
     1381 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
     1441 ggcttcctaa tcgacgacgc accggctgcc ggcggttgcc gggattcttt gcggattcga
     1501 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
     1561 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
     1621 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
     1681 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
     1741 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
     1801 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
     1861 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
     1921 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
     1981 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
     2041 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
     2101 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
     2161 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
     2221 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
     2281 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
     2341 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
     2401 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
     2461 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
     2521 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
     2581 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
     2641 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
     2701 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
     2761 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
     2821 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
     2881 gatttcatgc agccgcacgg ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     2941 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
     3001 gacgcttaga cacttaataa cacattgcgg acgtttttaa tgtactgggg tggattacgg
     3061 cgggggcatc ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga
     3121 aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca
     3181 tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag
     3241 tttatataca gctagagtcg aagtagtgat tggtaagtct gattgaccaa cgttttagag
     3301 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     3361 tcggtgcttt tttttactgt cgagtatctg gggcatcttc attcttaaga tatgaagata
     3421 atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta tatgggaaag
     3481 aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa aagtcccaca
     3541 tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag tagtgattgt
     3601 gagagattga gaaatcgagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     3661 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt ttactgtcga gtacaccggg
     3721 catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag
     3781 agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag
     3841 ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata
     3901 tacagctaga gtcgaagtag tgattgagac gtgtgtgaac agaaagtttt agagctagaa
     3961 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     4021 ctttttttta ctgtcgagta aagggggcat cttcattctt aagatatgaa gataatcttc
     4081 aaaaggcccc tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat
     4141 agtatttctt atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt
     4201 agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga ttgcagaacc
     4261 aagatatcca cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     4321 acttgaaaaa gtggcaccga gtcggtgctt ttttttactg tcgagtaaat cgggcatctt
     4381 cattcttaag atatgaagat aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc
     4441 aggcccattt atatgggaaa gaacaatagt atttcttata taggcccatt taagttgaaa
     4501 acaatcttca aaagtcccac atcgcttaga taagaaaacg aagctgagtt tatatacagc
     4561 tagagtcgaa gtagtgattg gagagagaga tgagctctag ttttagagct agaaatagca
     4621 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     4681 tttactgtcg agtatgaggg gcatcttcat tcttaagata tgaagataat cttcaaaagg
     4741 cccctgggaa tctgaaagaa gagaagcagg cccatttata tgggaaagaa caatagtatt
     4801 tcttatatag gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc gcttagataa
     4861 gaaaacgaag ctgagtttat atacagctag agtcgaagta gtgattggaa aatcgaatct
     4921 aaccgtgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     4981 aaaagtggca ccgagtcggt gctttttttt actgtcgagt atgtcgggcg cgggggcatc
     5041 ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga aagaagagaa
     5101 gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca tttaagttga
     5161 aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag tttatataca
     5221 gctagagtcg aagtagtgat tgctctcatc ctctaaccca agttttagag ctagaaatag
     5281 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     5341 tttttactgt cgagtatctg gggcatcttc attcttaaga tatgaagata atcttcaaaa
     5401 ggcccctggg aatctgaaag aagagaagca ggcccattta tatgggaaag aacaatagta
     5461 tttcttatat aggcccattt aagttgaaaa caatcttcaa aagtcccaca tcgcttagat
     5521 aagaaaacga agctgagttt atatacagct agagtcgaag tagtgattga tatactgccc
     5581 taaatgcggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     5641 gaaaaagtgg caccgagtcg gtgctttttt ttactgtcga gtacaccggg catcttcatt
     5701 cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag agaagcaggc
     5761 ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag ttgaaaacaa
     5821 tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga
     5881 gtcgaagtag tgattgcaac agcctggctc attgggtttt agagctagaa atagcaagtt
     5941 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttta
     6001 ctgtcgagta aagggggcat cttcattctt aagatatgaa gataatcttc aaaaggcccc
     6061 tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat agtatttctt
     6121 atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt agataagaaa
     6181 acgaagctga gtttatatac agctagagtc gaagtagtga ttgatatact gcccaaaatg
     6241 cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     6301 gtggcaccga gtcggtgctt ttttttactg tcgagtaaat cgggcatctt cattcttaag
     6361 atatgaagat aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc aggcccattt
     6421 atatgggaaa gaacaatagt atttcttata taggcccatt taagttgaaa acaatcttca
     6481 aaagtcccac atcgcttaga taagaaaacg aagctgagtt tatatacagc tagagtcgaa
     6541 gtagtgattg ataaagcttt tttgaacagg ttttagagct agaaatagca agttaaaata
     6601 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttactgtcg
     6661 agtatgaggg gcatcttcat tcttaagata tgaagataat cttcaaaagg cccctgggaa
     6721 tctgaaagaa gagaagcagg cccatttata tgggaaagaa caatagtatt tcttatatag
     6781 gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc gcttagataa gaaaacgaag
     6841 ctgagtttat atacagctag agtcgaagta gtgattggat gcagttgaat ctgacagttt
     6901 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     6961 ccgagtcggt gctttttttt actgtcgagt atgtcattgt agataattag gctaaactat
     7021 ccttaaggtg tctagataat taggctaaac tatccttaag gtgaggcgga tccctcaact
     7081 tttgattcgc tatttgcagt gcacctgtgg cgttcatcac atcttttgtg acactgtttg
     7141 cactggtcat tgctattaca aaggaccttc ctgatgttga aggagatcga aagtaagtaa
     7201 ctgcacgcat aaccattttc tttccgctct ttggctcaat ccatttgaca gtcaaagaca
     7261 atgtttaacc agctccgttt gatatattgt ctttatgtgt ttgttcaagc atgtttagtt
     7321 aatcatgcct ttgattgatc ttgaataggt tccaaatatc aaccctggca acaaaacttg
     7381 gagtgagaaa cattgcattc ctcggttctg gacttctgct agtaaattat gtttcagcca
     7441 tatcactagc tttctacatg cctcaggtga attcatctat ttccgtctta actatttcgg
     7501 ttaattaaag cacgaacacc attactgcat gtagaagctt gataaactat cgccaccaat
     7561 ttatttttgt tgcgatattg ttactttcct cagtatgcag ctttgaaaag accaaccctc
     7621 ttatccttta acaatgaaca ggtttttaga ggtagcttga tgattcctgc acatgtgatc
     7681 ttggcttcag gcttaatttt ccaggtaaag cattatgaga tactcttata tctcttacat
     7741 acttttgaga taatgcacaa gaacttcata actatatgct ttagtttctg catttgacac
     7801 tgccaaattc attaatctct aatatctttg ttgttgatct ttggtagaca tgggtactag
     7861 aaaaagcaaa ctacaccaag gtaaaatact tttgtacaaa cataaactcg ttatcacgga
     7921 acatcaatgg agtgtatatc taacggagtg tagaaacatt tgattattgc aggaagctat
     7981 ctcaggatat tatcggttta tatggaatct cttctacgca gagtatctgt tattcccctt
     8041 cctctagctt tcaatttcat ggtgaggata tgcagttttc tttgtatatc attcttcttc
     8101 ttctttgtag cttggagtca aaatcggttc cttcatgtac atacatcaag gatatgtcct
     8161 tctgaatttt tatatcttgc aataaaaatg cttgtaccaa ttgaaacacc agctttttga
     8221 gttctatgat cactgacttg gttctaacca aaaaaaaaaa atgtttaatt tacatatcta
     8281 aaagtaggtt tagggaaacc taaacagtaa aatatttgta tattattcga atttcactca
     8341 tcataaaaac ttaaattgca ccataaaatt ttgttttact attaatgatg taatttgtgt
     8401 aacttaagat aaaaataata ttccgtaagt taaccggcta aaaccacgta taaaccaggg
     8461 aacctgttaa accggttctt tactggataa agaaatgaaa gcccatgtag acagctccat
     8521 tagagcccaa accctaaatt tctcatctat ataaaaggag tgacattagg gtttttgttc
     8581 gtcctcttaa agcttctcgt tttctctgcc gtctctctca ttcgcgcgac gcaaacgatc
     8641 ttcaggtgat cttctttctc caaatcctct ctcataactc tgatttcgta cttgtgtatt
     8701 tgagctcacg ctctgtttct ctcaccacag ccccatggac tataaggacc acgacggaga
     8761 ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc caaagaagaa
     8821 gcggaaggtc ggtatccacg gagtcccagc agccgacaag aagtacagca tcggcctgga
     8881 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
     8941 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
     9001 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
     9061 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
     9121 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
     9181 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
     9241 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
     9301 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
     9361 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
     9421 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     9481 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     9541 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     9601 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     9661 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     9721 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     9781 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     9841 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     9901 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     9961 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
    10021 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
    10081 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
    10141 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
    10201 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
    10261 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
    10321 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
    10381 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
    10441 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
    10501 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
    10561 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
    10621 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
    10681 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
    10741 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
    10801 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
    10861 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
    10921 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
    10981 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
    11041 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
    11101 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
    11161 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
    11221 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
    11281 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
    11341 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga
    11401 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
    11461 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
    11521 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
    11581 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
    11641 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
    11701 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
    11761 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
    11821 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
    11881 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
    11941 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
    12001 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
    12061 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
    12121 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
    12181 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
    12241 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
    12301 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
    12361 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
    12421 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
    12481 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
    12541 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
    12601 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
    12661 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
    12721 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
    12781 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
    12841 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
    12901 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacaaaag
    12961 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagtaaaagg gtgggatatg
    13021 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
    13081 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
    13141 atctcattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
    13201 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
    13261 ataagtctgg aagcacctga cctaagctag cctagaaggg ctaagaccta agctagccta
    13321 gaatctgacc taagctagcc tagtgaggta tgtaggtata gtaacatgaa caagaacaga
    13381 ctcaagtaca agatcgcata cgaaaatgga aatggcaatg tcacttccac ataatcaaac
    13441 acgaatcctc atatcaacaa ggcctgagat tctaactagc tcataacaac ttagccaata
    13501 gttacttgag actaccaaat gtatgtagaa ctaaagacta agggacagag agttcgtcta
    13561 aacaggtgaa tctagtcgtt gttatctaat aaacaattca gccccaaatg cagaacacac
    13621 atagagctct ctattgattc aaattacgat ctgatactga taacgtctag atttttaggg
    13681 ttaaagcaat caatcacctg acgattcaag gtggttggat catgacgatt ccagaaaaca
    13741 tcaagcaagc tctcaaagct acactctttg ggatcatact gaactctaac aacctcgtta
    13801 tgtcccgtag tgccagtaca gacatcctcg taactcggat tgtgcacgat gccatggcta
    13861 tacccaacct cggtcttggt cacaccagga actctctggt aagctagctc cactccccag
    13921 aaacaaccgg cgccaaattg cgcgaattgc tgacctgatg acggaacatc atcgtcgggt
    13981 ccttgggcga ttgcggcgga agatgggtca gcttgggctt gaggacgaga gccgaatccg
    14041 agtctgttga aaaggttgtt cattggggat ttgtatacgg agattggtcg tcgagaggtt
    14101 tgagggaaag gacaaatggg tttggctctg gagaaagaga gtgcggcttt agagagagaa
    14161 ttgagaggtt tagagagaga tgcggcggcg atgagcgtgc cgaggagaga cgacgaggac
    14221 ctgcattatc aaagcagtga cgtggtgaaa tttggaactt ttaagaggca gatagattta
    14281 ttatttgtat ccattttctt cattgttcta gaatgtcgcg gaacaaattt taaaactaaa
    14341 tcctaaattt ttctaatttt gttgccaata gtggatatgt gggccgtata gaaggaatct
    14401 attgaaggct caaacccata ctgacgagcc caaaggttcg ttttgcgttt tatgtttcgg
    14461 ttcgatgcca acgccacatt ctgagctagg caaaaaacaa acgtgtcttt gaatagactc
    14521 ctctcgttaa cacatgcagc ggctgcatgg tgacgccatt aacacgtggc ctacaattgc
    14581 atgatgtctc cattgacacg tgacttctcg tctcctttct taatatatct aacaaacact
    14641 cctacctctt ccaaaatata tacacatctt tttgatcaat ctctcattca aaatctcatt
    14701 ctctctagta aacaagaaca aaaaaatggc ggatacagct agaggaaccc atcacgatat
    14761 catcggcagg gaccagtacc cgatgatggg ccgtgaccgt gaccagtacc agatgtccgg
    14821 acgaggatct gactactcca agtctaggca gattgctaaa gctgcaactg ctgtcacagc
    14881 tggtggttcc ctccttgttc tctccagcct tacccttgtt ggaactgtca tagctttgac
    14941 tgttgcaaca cctctgctcg ttatcttcag cccaatcctt gtcccggctc tcatcacagt
    15001 tgcactcctc atcaccggtt ttctttcctc tggagggttt ggcattgccg ctataaccgt
    15061 tttctcttgg atttacaagt aagcacacat ttatcatctt acttcataat tttgtgcaat
    15121 atgtgcatgc atgtgttgag ccagtagctt tggatcaatt tttttggtcg aataacaaat
    15181 gtaacaataa gaaattgcaa attctaggga acatttggtt aactaaatac gaaatttgac
    15241 ctagctagct tgaatgtgtc tgtgtatatc atctatatag gtaaaatgct tggtatgata
    15301 cctattgatt gtgaataggt acgcaacggg agagcaccca cagggatcag acaagttgga
    15361 cagtgcaagg atgaagttgg gaagcaaagc tcaggatctg aaagacagag ctcagtacta
    15421 cggacagcaa catactggtg gggaacatga ccgtgaccgt actcgtggtg gccagcacac
    15481 tactatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
    15541 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
    15601 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
    15661 taccagcttc atgtacggca gcagaacctt catcaaccac acccagggca tccccgactt
    15721 ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaggacgg
    15781 aggtgtgctg accgctactc aggacaccag cctccaggac ggctgcctca tctacaacgt
    15841 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
    15901 ctgggaagcc aacaccgaga tgctgtatcc agctgacgga ggactggaag gcagaagcga
    15961 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag
    16021 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact
    16081 ggaaagaatc aaggaggccg acaaagaaac ctacgtcgag cagcacgagg tggctgtggc
    16141 cagatactgc gacctcccta gcaaactggg gcacaagtga ttagagtttt tcagatccgc
    16201 gtttgtgttt tctgggtttc tcacttaagc gtctgcgttt tacttttgta ttgggtttgg
    16261 cgtttagtag tttgcggtag cgttcttgtt atgtgtaatt acgctttttc ttcttgcttc
    16321 agcagtttcg gttgaaatat aaatcgaatc aagtttcact ttatcagcgt tgttttaaat
    16381 tttggcatta aattggtgaa aattgcttca attttgtatc taaatagaag tgtctctggt
    16441 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
    16501 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
    16561 gtgccaacca caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    16621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    16681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    16741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    16801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    16861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    16921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    16981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    17041 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    17101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    17161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    17221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    17281 ggatcttcac ctagatcata aaatcattat ttgccatcca gctgatatcc cctatagtga
    17341 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
    17401 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
    17461 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
    17521 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
    17581 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
    17641 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
    17701 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
    17761 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
    17821 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
    17881 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt cttgctcagg cgcaatcacg
    17941 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
    18001 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
    18061 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
    18121 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
    18181 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
    18241 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
    18301 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
    18361 atgac
//
