LOCUS       Exported               15957 bp ds-DNA     circular SYN 09-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...9z1-iv6hodn
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15957
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
     promoter        382..580
                     /label=CMV promoter
     LTR             598..778
                     /label=5' LTR (truncated)
     misc_feature    825..950
                     /label=HIV-1 Psi
     misc_feature    1443..1676
                     /label=RRE
     misc_feature    2203..2320
                     /label=cPPT/CTS
     misc_feature    2371..3041
                     /label=UCOE
     promoter        3091..3302
                     /label=EF-1-alpha core promoter
     regulatory      3321..3326
                     /label=Kozak sequence
     CDS             3327..3383
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             3384..7484
                     /product="nickase mutant of the Cas9 endonuclease from 
                     theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(R221K N394K H840A)
                     /note="/Original_Translation=DKKYSIGLDIGTNSVGWAVITDEYKVPSKK
                     FKVLG 
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD 
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR 
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA 
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK 
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD 
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD 
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK 
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK 
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK 
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL 
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQ 
                     SGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPA 
                     IKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKE 
                     LGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLK 
                     DDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG 
                     GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS 
                     DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI 
                     AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF 
                     ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPT 
                     VAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII 
                     KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ 
                     KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL 
                     FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /note="/modified_by=chenp"
     CDS             7485..7586
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
     CDS             7587..9617
                     /label=RT codon optimized Genscript
                     /note="/created_by=jessiedavis"
     CDS             9630..9683
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/Original_Translation=KRTADGSEFEPKKKRKV"
     CDS             9684..9719
                     /label=GSG-cmycNLS
                     /note="/created_by=chenp"
                     /note="/modified_by=chenp"
     CDS             9720..9785
                     /label=Translation 7440-7577
     CDS             9729..9785
                     /label=P2A
     misc_feature    9786..10505
                     /label=GFP
     unsure          10506..10796
                     /label=LinkerKASH
                     /note="/created_by=lkoblan"
     misc_feature    10812..11400
                     /label=WPRE
     LTR             11472..11705
                     /label=3' LTR (Delta-U3)
     polyA_signal    11737..11961
                     /label=bGH poly(A) signal
     rep_origin      12007..12435
                     /label=f1 ori
     promoter        12449..12778
                     /label=SV40 promoter
     rep_origin      12629..12764
                     /label=SV40 ori
     promoter        12826..12873
                     /label=EM7 promoter
     CDS             12892..13266
                     /label=BleoR
     polyA_signal    13396..13517
                     /label=SV40 poly(A) signal
     primer_bind     complement(13566..13582)
                     /label=M13 rev
     protein_bind    13590..13606
                     /label=lac operator
     promoter        complement(13614..13620)
                     /label=lac promoter
     promoter        complement(13621..13638)
                     /label=lac promoter
     promoter        complement(13639..13644)
                     /label=lac promoter
     protein_bind    13659..13680
                     /label=CAP binding site
     rep_origin      complement(13968..14556)
                     /direction=LEFT
                     /label=ori
     CDS             complement(14727..15518)
                     /label=AmpR
     CDS             complement(15519..15587)
                     /label=AmpR
     promoter        complement(15588..15692)
                     /label=AmpR promoter
     unsure          join(15931..15957,1..19)
                     /note="/Color=#ff0000"
                     /note="/created_by=lkoblan"
                     /note="/modified_by=lkoblan"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaggtacc cgggaggtgg tccctgcagt tacgccaatg
     2401 ataacccccg ccagaaaaat cttagtagcc ttcccttttt gttttccgtg ccccaactcg
     2461 gcggattgac tcggcccctt ccggaaacac ccgaatcaac ttctagtcaa attattgttc
     2521 acgccgcaat gacccacccc tggcccgcgt ctgtggaact gacccctggt gtacaggaga
     2581 gttcgctgct gaaagtggtc ccaaaggggt actagttttt aagctcccaa ctccccctcc
     2641 cccagcgtct ggaggattcc acaccctcgc accgcagggg cgaggaagtg ggcggagtcc
     2701 ggttttggcg ccagccgctg aggctgccaa gcagaaaagc caccgctgag gagactccgg
     2761 tcactgtcct cgccccgcct cccccttccc tccccttggg gaccaccggg cgccacgccg
     2821 cgaacggtaa gtgccgcggt cgtcggcgcc tccgccctcc ccctagggcc ccaattccca
     2881 gcgggcgcgg cgcgcggccc ctccccccgc cgggcgcgcg cccgctgccc cgcccttcgt
     2941 ggccgcccgg cgtgggcggt gccacccctc cccccggcgg ccccgcgcgc agctcccggc
     3001 tccctccccc ttcggatgtg gcttgagctg taggcgcgga ggctagctag gtcttgaaag
     3061 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     3121 agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa
     3181 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     3241 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     3301 aggaccggtt ctagagcgct gccaccatga aacggacagc cgacggaagc gagttcgagt
     3361 caccaaagaa gaagcggaaa gtcgacaaga agtacagcat cggcctggac atcggcacca
     3421 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
     3481 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg
     3541 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
     3601 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
     3661 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     3721 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     3781 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     3841 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     3901 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     3961 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     4021 ccagactgag caagagcaga aagctggaaa atctgatcgc ccagctgccc ggcgagaaga
     4081 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     4141 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     4201 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     4261 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     4321 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     4381 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     4441 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca
     4501 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     4561 agagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     4621 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     4681 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     4741 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     4801 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc
     4861 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     4921 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa
     4981 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca
     5041 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     5101 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     5161 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     5221 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     5281 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     5341 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     5401 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     5461 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     5521 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     5581 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     5641 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     5701 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     5761 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     5821 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     5881 ccgactacga tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgaca
     5941 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     6001 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc
     6061 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     6121 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     6181 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga
     6241 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     6301 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     6361 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
     6421 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     6481 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     6541 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt
     6601 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata
     6661 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga
     6721 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
     6781 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
     6841 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
     6901 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
     6961 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
     7021 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga
     7081 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
     7141 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca
     7201 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct
     7261 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta
     7321 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga
     7381 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
     7441 gcctgtacga gacacggatc gacctgtctc agctgggagg tgactccggc ggaagctctg
     7501 gtggcagcaa gcggaccgcc gacggctctg aattcgagag ccctaagaag aaaagaaagg
     7561 tgagcggagg ctctagcggc ggaagcaccc tgaacattga agacgagtat agactgcatg
     7621 aaacaagcaa ggaacccgac gtgtccctgg gctccacctg gctgtccgac tttccccagg
     7681 cctgggccga gacaggagga atgggcctgg ccgtgcggca ggcacccctg atcatccctc
     7741 tgaaggccac ctctacaccc gtgagcatca agcagtaccc tatgtctcag gaggccagac
     7801 tgggcatcaa gcctcacatc cagaggctgc tggaccaggg catcctggtg ccatgccaga
     7861 gcccctggaa cacaccactg ctgcccgtga agaagccagg caccaatgac tatagacccg
     7921 tgcaggatct gagagaggtg aacaagaggg tggaggatat ccaccccacc gtgcccaacc
     7981 cttacaatct gctgtccggc ctgccccctt ctcaccagtg gtatacagtg ctggacctga
     8041 aggatgcctt cttttgtctg agactgcacc ctaccagcca gccactgttc gcctttgagt
     8101 ggagggaccc tgagatgggc atctctggcc agctgacctg gacacgcctg cctcagggct
     8161 tcaagaatag cccaacactg tttaacgagg ccctgcaccg cgacctggca gatttccgga
     8221 tccagcaccc agatctgatc ctgctgcagt acgtggacga tctgctgctg gccgccacca
     8281 gcgagctgga ttgccagcag ggaacacgcg ccctgctgca gaccctggga aacctgggat
     8341 atagggcatc cgccaagaag gcccagatct gtcagaagca ggtgaagtac ctgggctatc
     8401 tgctgaagga gggccagaga tggctgacag aggccaggaa ggagacagtg atgggccagc
     8461 caacacccaa gaccccaaga cagctgaggg agttcctggg caaagcagga ttttgcaggc
     8521 tgttcatccc aggattcgca gagatggcag cacctctgta cccactgacc aagccgggca
     8581 ccctgtttaa ttggggccct gaccagcaga aggcctatca ggagatcaag caggccctgc
     8641 tgacagcacc agccctgggc ctgccagacc tgaccaagcc tttcgagctg tttgtggatg
     8701 agaagcaggg ctacgccaag ggcgtgctga cccagaagct gggaccatgg agacggcccg
     8761 tggcctatct gtccaagaag ctggacccag tggcagcagg atggccacca tgcctgagga
     8821 tggtggcagc aatcgccgtg ctgacaaagg atgccggcaa gctgaccatg ggacagccac
     8881 tggtcatcct ggcaccacac gcagtggagg ccctggtgaa gcagcctcca gatcgctggc
     8941 tgtctaacgc ccggatgaca cactaccagg ccctgctgct ggacaccgat cgcgtgcagt
     9001 ttggccctgt ggtggccctg aatccagcca ccctgctgcc tctgccagag gagggcctgc
     9061 agcacaactg tctggacatc ctggcagagg cacacggaac aaggccagac ctgaccgatc
     9121 agcccctgcc tgacgccgat cacacatggt ataccgatgg aagctccctg ctgcaggagg
     9181 gccagaggaa ggcaggagca gcagtgacca cagagacaga agtgatctgg gccaaggccc
     9241 tgccagcagg cacatccgcc cagcgggccg agctgatcgc cctgacccag gccctgaaga
     9301 tggccgaggg caagaagctg aacgtgtaca cagactccag atatgccttc gccaccgcac
     9361 acatccacgg agagatctac aggcgccggg gctggctgac ctctgagggc aaggagatca
     9421 agaacaagga tgagatcctg gccctgctga aggccctgtt tctgcccaag cggctgagca
     9481 tcatccactg tcctggacac cagaagggac actccgccga ggcaaggggc aatcggatgg
     9541 ccgaccaggc cgccagaaag gctgctatta ctgaaactcc cgacacttcc actctgctga
     9601 ttgaaaactc ctccccttct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagt
     9661 ctcccaagaa gaagaggaaa gtcggctctg gccctgccgc taagagagtg aagctggacg
     9721 gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg
     9781 gaccgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     9841 tggacggcga cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca
     9901 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     9961 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
    10021 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
    10081 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
    10141 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
    10201 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
    10261 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
    10321 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
    10381 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
    10441 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
    10501 agtccagcgg aggctctggt gggtcttctg ggctgaggtc aagagaagaa gaggaggaga
    10561 cagattccag gatgcctcac ctggacagcc cgggaagtag ccagcctcga cgtagtttcc
    10621 tgtccagagt gatccgggct gctctacccc tgcagctgtt gcttctctta ctacttttgc
    10681 tggcctgtct cctcccagca tcagaagatg actacagctg cacgcaagcc aacaactttg
    10741 cgcggtcctt ctatcccatg ctgcgctaca ccaatggccc tccacccact taatagacgc
    10801 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
    10861 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    10921 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    10981 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    11041 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    11101 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    11161 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
    11221 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    11281 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    11341 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    11401 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    11461 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
    11521 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11581 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11641 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11701 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
    11761 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
    11821 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
    11881 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
    11941 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
    12001 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    12061 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    12121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    12181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    12241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    12301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    12361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    12421 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
    12481 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
    12541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    12601 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    12661 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
    12721 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    12781 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
    12841 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
    12901 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
    12961 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
    13021 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
    13081 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
    13141 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
    13201 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
    13261 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    13321 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    13381 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    13441 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    13501 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
    13561 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    13621 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    13681 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    13741 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    13801 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    13861 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    13921 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    13981 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    14041 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    14101 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    14161 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    14221 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    14281 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    14341 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    14401 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    14461 gttggtagct cttgatccgg caaacaaacc accgctacca gcggtggttt ttttgtttgc
    14521 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    14581 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    14641 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    14701 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    14761 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    14821 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    14881 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    14941 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    15001 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    15061 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    15121 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    15181 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    15241 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    15301 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    15361 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    15421 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    15481 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    15541 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    15601 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    15661 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    15721 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
    15781 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
    15841 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
    15901 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
