LOCUS       Exported                8214 bp ds-DNA     circular UNA 09-APR-2025
DEFINITION  natural circular DNA
ACCESSION   urn.local...9yo-iv6hnss
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    812..831
                     /label=BII(R)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BII(R)
                     (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    874..936
                     /label=5'ITR
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:13/-19;
                     31bp spacer (pRH148(DY001-W)_with_sa)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     5'ITR
                     (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    1112..1343
                     /label=Core Insulator
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Core
                     Insulator (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(1113..1343)
                     /label=Core Insulator
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Core
                     Insulator (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    1370..1396
                     /label=MC_gbpr65
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     MC_gbpr65 (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    1393..1633
                     /label=minUCOE
                     /label=nonstandard type:  chromatin
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minUCOE
                     (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    1582..1595
                     /label=CTCF binding site
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     CTCF
                     binding site (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    1634..2063
                     /label=UCOE
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     UCOE (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2065..2143
                     /label=EFS
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     origin (pRH148(DY001-W)_with_sa)"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; EFS
                     (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2144..3242
                     /label=hEF1alpha promoter
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     hEF1alpha promoter (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     gene            3272..3967
                     /label=BFP
     LTR             4047..4647
                     /label=wPRE
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     wPRE (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    4680..4810
                     /label=SV40 polyA
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SV40
                     polyA (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_RNA        5053..5135
                     /label=Insert pegRNA cassettes here
                     /note="/created_by=lkoblan"
     misc_feature    5058..5064
                     /label=paqCI
                     /note="Geneious type: primer_bind_reverse"
                     /note="/%GC=71.4"
                     /note="/Hairpin_Tm=None"
                     /note="/Self_Dimer_Tm=None"
                     /note="/Sequence=CACCTGC"
                     /note="/Tm=10.9"
                     /note="/created_by=lkoblan"
     misc_feature    5069..5072
                     /label=5' overhang
                     /note="Geneious type: primer_bind_reverse"
                     /note="/Sequence=GGAG"
                     /note="/created_by=lkoblan"
                     /note="/modified_by=lkoblan"
     misc_RNA        5073..5076
                     /label=5' overhang
                     /note="/created_by=lkoblan"
     misc_RNA        complement(5078..5083)
                     /label=bsmbI
                     /note="/created_by=lkoblan"
     misc_RNA        5104..5109
                     /label=bsmbI
                     /note="/created_by=lkoblan"
     misc_RNA        5111..5114
                     /label=3' overhang
                     /note="/created_by=lkoblan"
     misc_feature    5115..5118
                     /label=3' overhang
                     /note="Geneious type: primer_bind_reverse"
                     /note="/Sequence=TGTG"
                     /note="/created_by=lkoblan"
                     /note="/modified_by=lkoblan"
     misc_feature    5163..5393
                     /label=Core Insulator
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Core
                     Insulator (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    5668..5702
                     /label=3'ITR
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     3'ITR
                     (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
     CDS             complement(7357..8214)
                     /label=Amp
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; Amp
                     (pRH148(DY001-W)_with_sa)"
                     /note="/Transferred_From=pRH148(DY001-W)_with_sa"
                     /note="/Transferred_Similarity=100.00%"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccaagg atctgcgata tcgataagct
     1381 tgatatcgaa ttcgggaggt ggtccctgca gttacgccaa tgataacccc cgccagaaaa
     1441 atcttagtag ccttcccttt ttgttttccg tgccccaact cggcggattg actcggcccc
     1501 ttccggaaac acccgaatca acttctagtc aaattattgt tcacgccgca atgacccacc
     1561 cctggcccgc gtctgtggaa ctgacccctg gtgtacagga gagttcgctg ctgaaagtgg
     1621 tcccaaaggg gtactagttt ttaagctccc aactccccct cccccagcgt ctggaggatt
     1681 ccacaccctc gcaccgcagg ggcgaggaag tgggcggagt ccggttttgg cgccagccgc
     1741 tgaggctgcc aagcagaaaa gccaccgctg aggagactcc ggtcactgtc ctcgccccgc
     1801 ctcccccttc cctccccttg gggaccaccg ggcgccacgc cgcgaacggt aagtgccgcg
     1861 gtcgtcggcg cctccgccct ccccctaggg ccccaattcc cagcgggcgc ggcgcgcggc
     1921 ccctcccccc gccgggcgcg cgcccgctgc cccgcccttc gtggccgccc ggcgtgggcg
     1981 gtgccacccc tccccccggc ggccccgcgc gcagctcccg gctccctccc ccttcggatg
     2041 tggcttgagc tgtaggcgcg gaggggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     2101 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     2161 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     2221 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     2281 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     2341 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     2401 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     2461 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     2521 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     2581 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     2641 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     2701 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     2761 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     2821 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     2881 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     2941 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     3001 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     3061 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     3121 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     3181 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     3241 tggaattctc acgcgtctgc agctagctat gagcgagctg attaaggaga acatgcacat
     3301 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     3361 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     3421 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     3481 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     3541 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     3601 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     3661 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gaaaccctgt accccgctga
     3721 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     3781 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     3841 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     3901 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     3961 gcttaatctc gagaaaatgt ccaaggatgg taagaaaaag aagaagaagt caaaaaccaa
     4021 gtgtgttatc atgtaatgaa ggtaccgctt atcgataatc aacctctgga ttacaaaatt
     4081 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4141 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4201 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4261 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4321 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4381 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4441 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4501 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4561 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4621 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcttaatta agttaactaa
     4681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4801 atcatgtctg gaattgactc aaatgatgtc aattagtcta tcagaagctc atctggtctc
     4861 ccttccgggg gacaagacat ccctgtttaa tatttaaaca gcagtgttcc caaactgggt
     4921 tcttatatcc cttgctctgg tcaaccaggt tgcagggttt cctgtcctca caggaacgaa
     4981 gtccctaaag aaacagtggc agccaggttt agccccggaa ttgactggat tcctttttta
     5041 gggcccattg gtctagacac ctgcatgcgg agggagtgag acgtggctcg ttagatagtc
     5101 tagcgtctca ctaatgtgac ctgcaggtgg gatccattag gcggccgcgt ttaaacatgg
     5161 ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga
     5221 ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg
     5281 gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta
     5341 gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct
     5401 ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat
     5461 aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac
     5521 tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac
     5581 gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta
     5641 gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag
     5701 ggttaatcta gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc
     5761 caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg
     5821 aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac
     5881 gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt
     5941 tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg
     6001 tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg
     6061 gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc
     6121 ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt
     6181 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6241 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6301 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6361 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6421 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6481 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6601 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6661 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6721 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6781 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6841 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6901 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6961 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7021 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7081 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7381 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7441 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7681 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7741 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7801 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8041 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8101 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8161 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcat
//
