LOCUS       pHD-SPARC2-I-13x_lexAop-GCaMP8m_VK05        9656 bp    DNA     circular UNA 27-APR-2022
DEFINITION  injected construct.
ACCESSION   urn.local...i-eo0odml
VERSION     urn.local...i-eo0odml
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    860..951
                     /note="Amp Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp Promoter"
     misc_feature    1232..1254
                     /note="sgRNA Target Site"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sgRNA Target Site"
     misc_binding    1258..2277
                     /note="VK00005 HAL"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
     primer_bind     1906..1929
                     /note="VK00005_R4"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="VK00005 R4 primer bind"
     primer_bind     2026..2045
                     /note="VK00005_R3"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="VK00005 R3 primer bind"
     misc_structure  <2278..2295
                     /note="Insert Digestion Sites by Q5 SDM for F2R1"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Insert Digestion Sites by Q5 SDM for F2R1"
     regulatory      2302..2680
                     /note="13XLexAop"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="13XLexAop regulatory"
     regulatory      2689..2941
                     /note="HSP70 minimal Promoter"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="HSP70 minimal Promoter regulatory"
     regulatory      2942..>3019
                     /note="IVS [Split]"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="IVS [Split] regulatory"
     gene            3102..3139
                     /note="AttP38 according to Groth et al"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AttP38 according to Groth et al gene"
     intron          3242..3307
                     /note="small T Intron from
                     pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS Note - This
                     was taken from Nern et al., 2015 (PNAS)"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="intron"
     misc_feature    3437..3457
                     /note="SV40 NLS from
                     pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="misc"
     misc_feature    3458..3706
                     /note="In line with SV40 NLS"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="In line with SV40 NLS"
     regulatory      3729..3863
                     /note="SV40 Poly A"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 Poly A regulatory"
     gene            3875..3934
                     /note="AttP Wt according to Groth et al"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AttP Wt according to Groth et al gene"
     gap             4029..4171
                     /note="Fill 1 - Here are the randomly generated filler
                     sequences. They should be devoid of most major restriction
                     enzyme sites and start codons. The sequence in the middle
                     of each one is a six frame stop. Designed by Irving"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
     gene            4266..4335
                     /note="AttBWT according to Groth et al., 2000"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="gene"
     gene            4340..5608
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="6xHis-GCaMP8m"
     regulatory      5609..5836
                     /note="SV40 PolyA Signal"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 PolyA Signal regulatory"
     misc_RNA        5837..5901
                     /note="Build back of LoxP site for BsiWI cloning into
                     pHD-dAttP-3xP3DsRed-HAR1_HAL4_AmpR(AttP40)"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="RNA"
     misc_recomb     5862..5895
                     /note="LoxP Site"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="LoxP Site"
     misc_recomb     5903..5920
                     /note="AttP Site"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AttP Site"
     misc_recomb     5921..5954
                     /note="LoxP Site"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="LoxP Site"
     misc_feature    5961..6166
                     /note="3xP3 Enhancer + hsp70 min"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="3xP3 Enhancer + hsp70 min"
     gene            6186..6863
                     /note="DsRed"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="DsRed"
     misc_feature    join(6719..>7145,<8192..8485)
                     /note="[Split]"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="F2.2_Fwd13Seq"
     misc_recomb     7112..7145
                     /note="LoxP Site 2"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="LoxP Site 2"
     misc_binding    7146..8165
                     /note="VK00005 HAR"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
     primer_bind     complement(7250..7273)
                     /note="VK00005_F4"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="VK00005 F4 primer bind"
     primer_bind     complement(7384..7405)
                     /note="VK00005_F3"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="VK00005 F3 primer bind"
     misc_feature    complement(8167..8189)
                     /note="sgRNA Target Site"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sgRNA Target Site"
     misc_feature    8169..8191
                     /note="sgRNA cut site in reverse"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="sgRNA cut site in reverse"
     oriT            8945..9533
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="oriT"
ORIGIN      
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttcg cggatctcgc caagtgacgg ttggagctac
     1261 atagttaaat gttaaattaa acacaaaaac atatttaaag tacatataaa tacacataat
     1321 tattaatgaa gaaacctatg cttaaaagat tcaatgtttg attggcatct tagaaaacca
     1381 agcgaaaaat acaaaaaaaa atcaacaaac aaaaattatg atatattatt taaaagtaaa
     1441 gtatacattt acattacaga aaaacaaaag agaaaacttg cggtagcaac aaaactatta
     1501 tattaattac attttaatta tgctgtacta ttatgattat taattattat gattaattaa
     1561 ttacgatttt tatgcttaga caaaccaaca aaaaacaaat atgcaaaaac cattaaaaaa
     1621 aaaacaaaaa acaagcaaaa aattacactg gcgcagaaat ttgtattgtc aaatcaataa
     1681 aaaatttgtt taaaaatttc aaaagaaagc ctcttaagtt ttttcatttc aatttccttt
     1741 tcagttgaac actttttctt aaaatgttca gttttacgtt actttgcttt ggagtggtaa
     1801 gattttgggt ttcacttgat ttcattttcg ttttgactat cgtgctgtaa aaatatttac
     1861 taaattatat cgaaagtatt tatatcataa ttaaataaac aagaatttta tgtgtataaa
     1921 tgcgccacaa ggaaaatgtg taaaaacaaa aaccgtcagc ggtggcaaaa aaaaaaatgg
     1981 aaagagaagg ggaaatctac aagaaatatt atattctttt aaaatttatg cagcgtaagg
     2041 aagcgatggt tccaccaaaa accacgaaat taacaaagat atcacacttc aataattgac
     2101 tctatatata cacgccagca tatgtacgta gatgaaacga ttttggtgtc tgagtatgta
     2161 tgtatgtatg taggtgtatg taacattgtc cgtaactttt agccagaaaa aacggcagca
     2221 gaaaattgta attgtgtaac taaaagtgaa tattttatgt aaaaactatc tcagtcacgt
     2281 agtcgatcgc ggtacactag tgcatgcctg caggttactg tacatccata cagtaagtac
     2341 tgtacatcca tacagtaagt actgtacatc catacagtaa gtactgtaca tccatacagt
     2401 aagtactgta catccataca gtaagcggag actctagccc tagggcatgc ctgcaggtta
     2461 ctgtacatcc atacagtaag tactgtacat ccatacagta agtactgtac atccatacag
     2521 taagcggaga ctctagcgct agcgcatgcc tgcaggttac tgtacatcca tacagtaagt
     2581 actgtacatc catacagtaa gtactgtaca tccatacagt aagtactgta catccataca
     2641 gtaagtactg tacatccata cagtaagcgg agactctagc actagtgacg tcgagcgccg
     2701 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga
     2761 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc
     2821 tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa
     2881 ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata
     2941 gatctaaaag gtaggttcaa ccactgatgc ctaggcacac cgaaacgact aaccctaatt
     3001 cttatccttt acttcagact agtgcggccg ctaacgtaag ctagctagag gatctttgtg
     3061 aaggaacctt acggtaccaa tcaaaagatc tactccagtc cccaactggg gtaacctttg
     3121 agttctctca gttggggtaa cgtaagctag ctagaggatc tttgtgaagg aaccttactt
     3181 gcggccgctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     3241 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     3301 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     3361 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     3421 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     3481 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     3541 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     3601 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     3661 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3721 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3781 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3841 gtatcttatc atgtctggat cgatctggcc ggcgggagta gtgccccaac tggggtaacc
     3901 tttgagttct ctcagttggg ggcgtagggt cggggtcggc atggcatctc cacctcctcg
     3961 cggtccgacc tgggcatccg aaggaggacg tcgtccactc ggatggctaa gggagagctc
     4021 ggatccgggc aatacgaggt actggcagac acgcagtgtc gaacaacacc tgacctaacg
     4081 gtaagatgag tagataactc aactacttaa atcctggcct gcttgatatc tcgtagcgtt
     4141 cttagagatc gacgaactgt ttcacgaccg ggtcggcatg gcatctccac ctcctcgcgg
     4201 tccgacctgg gcatccgaag gaggacgtcg tccactcgga tggctaaggg agagctcgga
     4261 tccctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
     4321 cacctcaccc atcgtcgaca tgcaccacca ccatcatcat accaggcgga aaaagacctt
     4381 caaagaggtc gccacagccg tgaaaatcat cggccgattg atgggattga agattaacgt
     4441 gtatattaag gccgataaac agaagaacgg catcaaggcc aatttccata tacggcacaa
     4501 catcgaagac ggaggcgtcc agctggccta ccattaccag cagaacacac ctatcggcga
     4561 tggaccggtg ctgttgccgg ataatcacta cctgagtgtg gaaagtaagt tgtcgaagga
     4621 ccctaacgaa aaacgcgacc acatggtgtt gctcgaattc gtgacggcgg ccggaatcac
     4681 actgggtatg gacgaactgt acaagggcgg aaccggagga tcgatggtaa gcaaaggcga
     4741 ggagctgttt accggcgtgg tgccgatttt ggtggagttg gacggcgatg tcaacggcca
     4801 caagttcagc gtgagcggtg agggtgaggg cgatgcaacc tacggcaaac tgacattgaa
     4861 attcatctgc accacgggaa aactcccggt tccatggcca acactggtca ccactctgac
     4921 ttatggcgtt cagtgcttta gccggtaccc cgaccatatg aaacagcatg acttctttaa
     4981 gtccgcgatg cccgagggtt acatccaaga gcgaacaatc ttcttcaagg atgacggcaa
     5041 ttacaagacg cgcgcagaag tcaagttcga gggcgatacc ctggtgaaca ggattgaatt
     5101 gaaaggcatt gactttaagg aggatggaaa catcctgggc cataagctgg agtacaacct
     5161 gccggaccag ctgacagagg agcaaatcgc ggagtacaag gaagccttta gcctgttcga
     5221 caaggacggt gatggcacga ttaccacaaa agagttgggc accgtcatgc gcagtctggg
     5281 acaaaatcct actgaagctg agctgcagga catgattaac gaggtcgatg cagatggcga
     5341 cggcactatc gactttcccg agttcttgac aatgatggcc cgcaagatga agtaccggga
     5401 taccgaggag gagatccgcg aagcctttgg cgtgttcgat aaagacggca atggctacat
     5461 tagcgctgcg gagctgcgcc atgtcatgac caacttgggc gagaagctca cggacgagga
     5521 agtggacgaa atgatacgcg aagcggacat cgatggcgat ggccaggtca actatgagga
     5581 attcgttcaa atgatgaccg caaagtaatc ataatcagcc ataccacatt tgtagaggtt
     5641 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     5701 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5761 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5821 atcaatgtat cttagtcgac tgaggcactg cctcgagaga taacttcgta taatgtatgc
     5881 tatacgaagt tatcgtacgc tctcagttgg gggcgtagat aacttcgtat aatgtatgct
     5941 atacgaagtt atcgtacggg atctaattca attagagact aattcaatta gagctaattc
     6001 aattaggatc caagcttatc gatttcgaac cctcgaccgc cggagtataa atagaggcgc
     6061 ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa
     6121 gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tcggctcgaa gccggtcgcc
     6181 accatggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
     6241 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6301 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     6361 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
     6421 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6481 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tccaggacgg ctccttcatc
     6541 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag
     6601 actatgggct gggaggcgtc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     6661 gagatccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
     6721 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg
     6781 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg cgccgagggc
     6841 cgccaccacc tgttcctgta ggggccgcga ctctagatca taatcagcca taccacattt
     6901 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     6961 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     7021 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7081 tccaaactca tcaatgtatc ttaaccggta taacttcgta taatgtatgc tatacgaagt
     7141 tattgggcga tcatgcgtat atatgtttat ggtggtcaag atgatgatga taatggtgat
     7201 ggattctgat agggaaatcc aaccgaacga cggcagcgac aactgaaaca atgaaattgc
     7261 gacaattaag cgtaacgtaa cgtaaagtat atgtaaaacg tatccgtaaa cagccgtaag
     7321 agtttaaagt aaagtaatag aaactaaaac attgaaaggg aatataatgc aactttgagg
     7381 caactccagc tgaggcatat gaaataccgt atgaataata tctattaata tatatatata
     7441 tacacatgaa tatattttca aagacagtca caaatcaaat tgcggcaaaa aaaagagttt
     7501 ttaaacatga gaatttggca aaaatcgaat aaaatatgag aagaatgata cgggaggaaa
     7561 aggaaatgtt tcgaaaattg attgaaactc cataaaaaat aaaaattaac agaaaatttg
     7621 tatttgtgaa ataaaataat attacagttt gttttaaatg gaaacacaat gaaaaccccc
     7681 cagaatgaaa tatgtgaata aaatattgaa aataagtatt taaaatatat caacaaaaat
     7741 tatgaaaaac aaatcgtttg tgttgttatt tggctttggt aatgcttttc agaatcgtaa
     7801 gtactaagta ctttggatcc tttaaatgtc atttatttta aaaaatgaag aatgaacgca
     7861 acttatggag ctgattaatg ctatcaccta gggctaaaga acaatgtcag ccattcttaa
     7921 taacccttgg aaacactcat cgtttggtgc cctctcccct tcaggcgatc gttcattact
     7981 atatccgtag gcgccaaaag cttatcacgc acacagccac gtttttttgt gctcgccgag
     8041 aatagcgatt attttcaatg tcgcggctgt ctgtcctagg cttttgttgg cgctgggcaa
     8101 gtgtgttttg gaattttgcc cgtgaccttg aggcgataag ataagagtcg cgaaatgaat
     8161 ttgagcccaa ccgtcacttg gcgagatccg tcaatcgagt tcaagggcga cacaaaattt
     8221 attctaaatg cataataaat actgataaca tcttatagtt tgtattatat tttgtattat
     8281 cgttgacatg tataattttg atatcaaaaa ctgattttcc ctttattatt ttcgagattt
     8341 attttcttaa ttctctttaa caaactagaa atattgtata tacaaaaaat cataaataat
     8401 agatgaatag tttaattata ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag
     8461 tgcgttgctt ttttctcatt tataaggtta aataattctc atatatcaag caaagtgaca
     8521 ggcgccctta aatattctga caaatgctct ttccctaaac tccccccata aaaaaacccg
     8581 ccgaagcggg tttttacgtt atttgcggat taacgattac tcgttatcag aaccgcccag
     8641 ggggcccgag cttaagactg gccgtcgttt tacaacacag aaagagtttg tagaaacgca
     8701 aaaaggccat ccgtcagggg ccttctgctt agtttgatgc ctggcagttc cctactctcg
     8761 ccttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8821 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9121 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9361 gctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9541 ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag
     9601 ggattttggt catgagcttg cgccgtcccg tcaagtcagc gtaatgctct gctttt
//
