LOCUS       Exported                7003 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN21A(pF-47nA)-WDR5      5707 bp    DNA   circular     
                  10/21/2016

            DEFINITION  Current Name: pFN21A (pF-47nA) - WDR5 Old/Alternate 
            Name(s):BIG-3 (insert) SWD3 (insert) WD repeat domain 5 (insert) 
            WDR5 (insert) pF-47nA (backbone)
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     misc_feature    992..997
                     /label=6base
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1067..4309
                     /label=Complete ORF
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:43:25 AM"
     misc_feature    1067..1957
                     /label=HT7
     misc_feature    complement(1859..1864)
                     /label=6base
     misc_feature    1958..1996
                     /label=HT7 Linker N terminal
     misc_feature    1970..1990
                     /label=TEV protease recognition sequence
     misc_feature    1997..2000
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2001..4307
                     /label=BRAF insert
                     /note="Recieved from Kazusa in April 2010. Entered into 
                     database semi automatically by Ethan in May 2010."
     misc_feature    2001..2004
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4304..4307
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4308..4441
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     misc_feature    4308..4311
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    4463..4684
                     /label=Sv40 late polyadenylation signal
     CDS             4945..5805
                     /label=beta-lactamase
     rep_origin      5960..5996
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6667..6952
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgccatggc ggcgctgagc ggtggcggtg gtggcggcgc
     2041 ggagccgggc caggctctgt tcaacgggga catggagccc gaggccggcg ccggcgccgg
     2101 cgccgcggcc tcttcggctg cggaccctgc cattccggag gaggtgtgga atatcaaaca
     2161 aatgattaag ttgacacagg aacatataga ggccctattg gacaaatttg gtggggagca
     2221 taatccacca tcaatatatc tggaggccta tgaagaatac accagcaagc tagatgcact
     2281 ccaacaaaga gaacaacagt tattggaatc tctggggaac ggaactgatt tttctgtttc
     2341 tagctctgca tcaatggata ccgttacatc ttcttcctct tctagccttt cagtgctacc
     2401 ttcatctctt tcagtttttc aaaatcccac agatgtggca cggagcaacc ccaagtcacc
     2461 acaaaaacct atcgttagag tcttcctgcc caacaaacag aggacagtgg tacctgcaag
     2521 gtgtggagtt acagtccgag acagtctaaa gaaagcactg atgatgagag gtctaatccc
     2581 agagtgctgt gctgtttaca gaattcagga tggagagaag aaaccaattg gttgggacac
     2641 tgatatttcc tggcttactg gagaagaatt gcatgtggaa gtgttggaga atgttccact
     2701 tacaacacac aactttgtac gaaaaacgtt tttcacctta gcattttgtg acttttgtcg
     2761 aaagctgctt ttccagggtt tccgctgtca aacatgtggt tataaatttc accagcgttg
     2821 tagtacagaa gttccactga tgtgtgttaa ttatgaccaa cttgatttgc tgtttgtctc
     2881 caagttcttt gaacaccacc caataccaca ggaagaggcg tccttagcag agactgccct
     2941 aacatctgga tcatcccctt ccgcacccgc ctcggactct attgggcccc aaattctcac
     3001 cagtccgtct ccttcaaaat ccattccaat tccacagccc ttccgaccag cagatgaaga
     3061 tcatcgaaat caatttgggc aacgagaccg atcctcatca gctcccaatg tgcatataaa
     3121 cacaatagaa cctgtcaata ttgatgactt gattagagac caaggatttc gtggtgatgg
     3181 aggatcaacc acaggtttgt ctgctacccc ccctgcctca ttacctggct cactaactaa
     3241 cgtgaaagcc ttacagaaat ctccaggacc tcagcgagaa aggaagtcat cttcatcctc
     3301 agaagacagg aatcgaatga aaacacttgg tagacgggac tcgagtgatg attgggagat
     3361 tcctgatggg cagattacag tgggacaaag aattggatct ggatcatttg gaacagtcta
     3421 caagggaaag tggcatggtg atgtggcagt gaaaatgttg aatgtgacag cacctacacc
     3481 tcagcagtta caagccttca aaaatgaagt aggagtactc aggaaaacac gacatgtgaa
     3541 tatcctactc ttcatgggct attccacaaa gccacaactg gctattgtta cccagtggtg
     3601 tgagggctcc agcttgtatc accatctcca tatcattgag accaaatttg agatgatcaa
     3661 acttatagat attgcacgac agactgcaca gggcatggat tacttacacg ccaagtcaat
     3721 catccacaga gacctcaaga gtaataatat atttcttcat gaagacctca cagtaaaaat
     3781 aggtgatttt ggtctagcta cagtgaaatc tcgatggagt gggtcccatc agtttgaaca
     3841 gttgtctgga tccattttgt ggatggcacc agaagtcatc agaatgcaag ataaaaatcc
     3901 atacagcttt cagtcagatg tatatgcatt tggaattgtt ctgtatgaat tgatgactgg
     3961 acagttacct tattcaaaca tcaacaacag ggaccagata atttttatgg tgggacgagg
     4021 atacctgtct ccagatctca gtaaggtacg gagtaactgt ccaaaagcca tgaagagatt
     4081 aatggcagag tgcctcaaaa agaaaagaga tgagagacca ctctttcccc aaattctcgc
     4141 ctctattgag ctgctggccc gctcattgcc aaaaattcac cgcagtgcat cagaaccctc
     4201 cttgaatcgg gctggtttcc aaacagagga ttttagtcta tatgcttgtg cttctccaaa
     4261 aacacccatc caggcagggg gatatggtgc gtttcctgtc cacgtttaaa cgaattcggg
     4321 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta
     4381 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     4441 cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     4501 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4561 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4621 tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt
     4681 ggtaaaatcg aattctaatg gatcctcttt gcgcttgcgt tttcccttgt ccagatagcc
     4741 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtaatg
     4801 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac tttcaaagtt
     5161 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5341 gccaacttac ttctgacaac tatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaattcga aatgaccgac
     5821 caagcgacgc ccaaccggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6361 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6541 aaggatttca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6661 tatagtccgg aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat
     6721 gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca
     6781 taggtattca tacggttaaa atttatcagg cgcgattgcg gcagtttttc gggtggtttg
     6841 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca
     6901 attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
     6961 agtactccgg ccacgatgcg tccggcgtag aggatcgaga tct
//
