LOCUS       Exported                5812 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-31Kp-MAP2K1      5812 bp    DNA   circular           
            5/31/2016

            DEFINITION  Current Name: pF-31Kp - MAP2K1
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="synthetic DNA construct"
                     /label=pF-31Kp-MEK1 (MAP2K1)
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from %22CMV 
                     Promoter%22 to %22CMV immediate-early enhancer/promoter%22 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..2783
                     /label=Complete ORF
     CDS             1065..1577
                     /label=N terminal NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1594..2781
                     /label=MAP2K1 insert
     misc_feature    2778..2785
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2782..2915
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     %22AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC 
                     AAGCTGATCCGGCTGCTAACAAAGC%22 at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    2937..3158
                     /label=Sv40 late polyadenylation signal
     promoter        3257..3675
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(3330..3566)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      3573..3638
                     /label=Sv40 Min Ori
     promoter        3683..3749
                     /label=EM7 bacterial promoter
     CDS             3763..4557
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    4621..4669
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gcccaagaag aagccgacgc
     1621 ccatccagct gaacccggcc cccgacggct ctgcagttaa cgggaccagc tctgcggaga
     1681 ccaacttgga ggccttgcag aagaagctgg aggagctaga gcttgatgag cagcagcgaa
     1741 agcgccttga ggcctttctt acccagaagc agaaggtggg agaactgaag gatgacgact
     1801 ttgagaagat cagtgagctg ggggctggca atggcggtgt ggtgttcaag gtctcccaca
     1861 agccttctgg cctggtcatg gccagaaagc taattcatct ggagatcaaa cccgcaatcc
     1921 ggaaccagat cataagggag ctgcaggttc tgcatgagtg caactctccg tacatcgtgg
     1981 gcttctatgg tgcgttctac agcgatggcg agatcagtat ctgcatggag cacatggatg
     2041 gaggttctct ggatcaagtc ctgaagaaag ctggaagaat tcctgaacaa attttaggaa
     2101 aagttagcat tgctgtaata aaaggcctga catatctgag ggagaagcac aagatcatgc
     2161 acagagatgt caagccctcc aacatcctag tcaactcccg tggggagatc aagctctgtg
     2221 actttggggt cagcgggcag ctcatcgact ccatggccaa ctccttcgtg ggcacaaggt
     2281 cctacatgtc gccagaaaga ctccagggga ctcattactc tgtgcagtca gacatctgga
     2341 gcatgggact gtctctggta gagatggcgg ttgggaggta tcccatccct cctccagatg
     2401 ccaaggagct ggagctgatg tttgggtgcc aggtggaagg agatgcggct gagaccccac
     2461 ccaggccaag gacccccggg aggcccctta gctcatacgg aatggacagc cgacctccca
     2521 tggcaatttt tgagttgttg gattacatag tcaacgagcc tcctccaaaa ctgcccagtg
     2581 gagtgttcag tctggaattt caagattttg tgaataaatg cttaataaaa aaccccgcag
     2641 agagagcaga tttgaagcaa ctcatggttc atgcttttat caagagatct gatgctgagg
     2701 aagtggattt tgcaggttgg ctctgctcca ccatcggcct taaccagccc agcacaccaa
     2761 cccatgctgc tggcgtcgtt taaacgaatt cgggctcggt acccggggat cctctagagt
     2821 cgacctgcag gcatgcaagc tgatccggct gctaacaaag cccgaaagga agctgagttg
     2881 gctgctgcca ccgctgagca ataactagca taaccccttg gggcggccgc ttcgagcaga
     2941 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3001 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3061 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     3121 ggttttttta agcaagtaaa acctctacaa atgtggtaaa atcgaatttt aacaaaatat
     3181 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3241 ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
     3301 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     3361 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3421 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3481 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3541 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3601 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3661 ttttgcaaaa agcttaatta actgttgaca attaatcatc ggcatagtat atcggcatag
     3721 tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgattga acaagatgga
     3781 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     3841 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     3901 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     3961 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4021 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4081 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     4141 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     4201 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     4261 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     4321 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     4381 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     4441 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4501 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     4561 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgatggccgc
     4621 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagcg
     4681 ataaggatcc gctcatgcga tgataagctg tcaaacatga gaattacaac ttatatcgta
     4741 tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt
     4801 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
     4861 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
     4921 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
     4981 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
     5041 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
     5101 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     5161 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     5221 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc
     5281 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     5341 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     5401 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     5461 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     5521 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca
     5581 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctagcgctg
     5641 atgtccggcg gtgcttttgc cgttacgcac caccccgtca tgagattacg cgttttagcg
     5701 gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt agccatcgag
     5761 ataaaccgca gtactccggc cacgatgcgt ccggcgtaga ggatcgagat ct
//
