LOCUS       Exported                5258 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pRSG118 (C-terminal HiBiT Flexi entry vector), designed by Chris 
            Eggers

            

            Synthesize the SacI/XhoI fragment and insert it into pNB51.

            

            Or...synthesize the SacI/XbaI fragment and insert it into pNB2K.
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from %22CMV 
                     Promoter%22 to %22CMV immediate-early enhancer/promoter%22 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     CDS             1065..2180
                     /label=CDK9
     misc_feature    2185..2249
                     /label=Untitled
     CDS             2190..2213
                     /codon_start=1
                     /label=8GS linker
                     /note="8GS linker"
                     /translation="GSSGGSSG"
     CDS             2214..2246
                     /codon_start=1
                     /label=HiBiT
                     /note="HiBiT"
                     /translation="VSGWRLFKKIS"
     polyA_signal    2383..2604
                     /label=Sv40 late polyadenylation signal
     promoter        2703..3121
                     /label=SV40 promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3129..3195
                     /label=EM7 bacterial promoter
     CDS             3209..4003
                     /label=NeoR / KanR
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    4067..4115
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    4158..5080
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    4335
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggcg aagcagtacg
     1081 actcggtgga gtgccctttt tgtgatgaag tttccaaata cgagaagctc gccaagatcg
     1141 gccaaggcac cttcggggag gtgttcaagg ccaggcaccg caagaccggc cagaaggtgg
     1201 ctctgaagaa ggtgctgatg gaaaacgaga aggaggggtt ccccattaca gccttgcggg
     1261 agatcaagat ccttcagctt ctaaaacacg agaatgtggt caacttgatt gagatttgtc
     1321 gaaccaaagc ttccccctat aaccgctgca agggtagtat atacctggtg ttcgacttct
     1381 gcgagcatga ccttgctggg ctgttgagca atgttttggt caagttcacg ctgtctgaga
     1441 tcaagagggt gatgcagatg ctgcttaacg gcctctacta catccacaga aacaagatcc
     1501 tgcataggga catgaaggct gctaatgtgc ttatcactcg tgatggggtc ctgaagctgg
     1561 cagactttgg gctggcccgg gccttcagcc tggccaagaa cagccagccc aaccgctaca
     1621 ccaaccgtgt ggtgacactc tggtaccggc ccccggagct gttgctcggg gagcgggact
     1681 acggcccccc cattgacctg tggggtgctg ggtgcatcat ggcagagatg tggacccgca
     1741 gccccatcat gcagggcaac acggagcagc accaactcgc cctcatcagt cagctctgcg
     1801 gctccatcac ccctgaggtg tggccaaacg tggacaacta tgagctgtac gaaaagctgg
     1861 agctggtcaa gggccagaag cggaaggtga aggacaggct gaaggcctat gtgcgtgacc
     1921 catacgcact ggacctcatc gacaagctgc tggtgctgga ccctgcccag cgcatcgaca
     1981 gcgatgacgc cctcaaccac gacttcttct ggtccgaccc catgccctcc gacctcaagg
     2041 gcatgctctc cacccacctg acgtccatgt tcgagtactt ggcaccaccg cgccggaagg
     2101 gcagccagat cacccagcag tccaccaacc agagtcgcaa tcccgccacc accaaccaga
     2161 cggagtttga gcgcgtcttc gtttctcaag gcagttcagg tggctcgagc ggtgtgagcg
     2221 gctggcggct gttcaagaag attagctaag gccgcgactc tagagtcgac ctgcaggcat
     2281 gcaagctgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     2341 tgagcaataa ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca
     2401 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2461 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2521 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca
     2581 agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt
     2641 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat
     2701 ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     2761 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     2881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2941 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3001 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3061 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3121 taattaactg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     3181 gtgaggaact aaacccagga ggcagatcat gattgaacaa gatggattgc acgcaggttc
     3241 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3301 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3361 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3421 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3481 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3541 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3601 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3661 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3721 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     3781 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     3841 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     3901 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3961 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     4021 gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt
     4081 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatccgctc
     4141 atgcgatgat aagctgtcaa acatgagaat tacaacttat atcgtatggg gctgacttca
     4201 ggtgctacat ttgaagagat aaattgcact gaaatctaga aatattttat ctgattaata
     4261 agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa
     4321 accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt
     4381 aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg
     4441 catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt
     4501 tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga
     4561 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
     4621 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     4681 aggcaggaac aggagagcgc acgagggagc cgccaggggg aaacgcctgg tatctttata
     4741 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg
     4801 ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt
     4861 cctggcatct tccaggaaat ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg
     4921 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt atcacatatt
     4981 ctgctgacgc accggtgcag ccttttttct cctgccacat gaagcacttc actgacaccc
     5041 tcatcagtgc caacatagta agccagtata cactccgcta gcgctgatgt ccggcggtgc
     5101 ttttgccgtt acgcaccacc ccgtcatgag attacgcgtt ttagcggtgc gtacaattaa
     5161 gggattatgg taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagtac
     5221 tccggccacg atgcgtccgg cgtagaggat cgagatct
//
