LOCUS       Exported                6132 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pRSG118 (C-terminal HiBiT Flexi entry vector), designed by Chris 
            Eggers

            

            Synthesize the SacI/XhoI fragment and insert it into pNB51.

            

            Or...synthesize the SacI/XbaI fragment and insert it into pNB2K.
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from %22CMV 
                     Promoter%22 to %22CMV immediate-early enhancer/promoter%22 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..2918
                     /label=BIRC2
                     /note="Created by Kazusa. Available from Kazusa. Data 
                     recieved from Kazusa 3/2009. Entered by Ethan 5/2009.  End 
                     definitions corrected 5/2010. Name changed from KIBB3090 to
                     BIRC2 by Ethan 5/17/2010. (note added in estrauss by 
                     5/17/2010 3:01:01 PM"
     misc_feature    2923..2987
                     /label=Untitled
     CDS             2928..2951
                     /codon_start=1
                     /label=8GS linker
                     /note="8GS linker"
                     /translation="GSSGGSSG"
     CDS             2952..2984
                     /codon_start=1
                     /label=HiBiT
                     /note="HiBiT"
                     /translation="VSGWRLFKKIS"
     polyA_signal    3121..3342
                     /label=Sv40 late polyadenylation signal
     promoter        3441..3859
                     /label=SV40 promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3867..3933
                     /label=EM7 bacterial promoter
     CDS             3947..4741
                     /label=NeoR / KanR
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    4805..4853
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5089..5125
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    5796..6081
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcac aaaactgcct
     1081 cccaaagact tttcccaggt ccctcgtatc aaaacattaa gagtataatg gaagatagca
     1141 cgatcttgtc agattggaca aacagcaaca aacaaaaaat gaagtatgac ttttcctgtg
     1201 aactctacag aatgtctaca tattcaactt tccccgccgg ggtgcctgtc tcagaaagga
     1261 gtcttgctcg tgctggtttt tattatactg gtgtgaatga caaggtcaaa tgcttctgtt
     1321 gtggcctgat gctggataac tggaaactag gagacagtcc tattcaaaag cataaacagc
     1381 tatatcctag ctgtagcttt attcagaatc tggtttcagc tagtctggga tccacctcta
     1441 agaatacgtc tccaatgaga aacagttttg cacattcatt atctcccacc ttggaacata
     1501 gtagcttgtt cagtggttct tactccagcc tttctccaaa ccctcttaat tctagagcag
     1561 ttgaagacat ctcttcatcg aggactaacc cctacagtta tgcaatgagt actgaagaag
     1621 ccagatttct tacctaccat atgtggccat taactttttt gtcaccatca gaattggcaa
     1681 gagctggttt ttattatata ggacctggag atagggtagc ctgctttgcc tgtggtggga
     1741 agctcagtaa ctgggaacca aaggatgatg ctatgtcaga acaccggagg cattttccca
     1801 actgtccatt tttggaaaat tctctagaaa ctctgaggtt tagcatttca aatctgagca
     1861 tgcagacaca tgcagctcga atgagaacat ttatgtactg gccatctagt gttccagttc
     1921 agcctgagca gcttgcaagt gctggttttt attatgtggg tcgcaatgat gatgtcaaat
     1981 gcttttgttg tgatggtggc ttgaggtgtt gggaatctgg agatgatcca tgggtagaac
     2041 atgccaagtg gtttccaagg tgtgagttct tgatacgaat gaaaggccaa gagtttgttg
     2101 atgagattca aggtagatat cctcatcttc ttgaacagct gttgtcaact tcagatacca
     2161 ctggagaaga aaatgctgac ccaccaatta ttcattttgg acctggagaa agttcttcag
     2221 aagatgctgt catgatgaat acacctgtgg ttaaatctgc cttggaaatg ggctttaata
     2281 gagacctggt gaaacaaaca gttcaaagta aaatcctgac aactggagag aactataaaa
     2341 cagttaatga tattgtgtca gcacttctta atgctgaaga tgaaaaaaga gaagaggaga
     2401 aggaaaaaca agctgaagaa atggcatcag atgatttgtc attaattcgg aagaacagaa
     2461 tggctctctt tcaacaattg acatgtgtgc ttcctatcct ggataatctt ttaaaggcca
     2521 atgtaattaa taaacaggaa catgatatta ttaaacaaaa aacacagata cctttacaag
     2581 cgagagaact gattgatacc attttggtta aaggaaatgc tgcggccaac atcttcaaaa
     2641 actgtctaaa agaaattgac tctacattgt ataagaactt atttgtggat aagaatatga
     2701 agtatattcc aacagaagat gtttcaggtc tgtcactgga agaacaattg aggaggttgc
     2761 aagaagaacg aacttgtaaa gtgtgtatgg acaaagaagt ttctgttgta tttattcctt
     2821 gtggtcatct ggtagtatgc caggaatgtg ccccttctct aagaaaatgc cctatttgca
     2881 ggggtataat caagggtact gttcgtacat ttctctctgt ttctcaaggc agttcaggtg
     2941 gctcgagcgg tgtgagcggc tggcggctgt tcaagaagat tagctaaggc cgcgactcta
     3001 gagtcgacct gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga
     3061 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag
     3121 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3181 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3241 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     3301 gggaggtttt tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa
     3361 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3421 cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     3481 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3601 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3661 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3721 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3781 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3841 aggcttttgc aaaaagctta attaactgtt gacaattaat catcggcata gtatatcggc
     3901 atagtataat acgacaaggt gaggaactaa acccaggagg cagatcatga ttgaacaaga
     3961 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4021 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4081 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     4141 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4201 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4261 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4321 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4381 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4441 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     4501 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4561 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4621 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4681 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4741 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg
     4801 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     4861 agcgataagg atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     4921 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacccggtat cagctcactc
     4981 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5041 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5101 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5161 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5221 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5281 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5341 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5401 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5461 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5521 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5581 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5641 ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc
     5701 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5761 atcaaaaagg atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg
     5821 atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg
     5881 ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc
     5941 gcgattgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     6001 gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt
     6061 ctgccctcgt agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga
     6121 ggatcgagat ct
//
