LOCUS       Exported                6354 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC27K(pFC27K)      5527 bp    DNA   circular           
            3/27/2014

            DEFINITION  Current Name: pFC27K (pFC27K) Old/Alternate 
            Name(s):pFC27K (backbone)
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..2267
                     /label=BRD4 (49-460)
                     /note="BRD4 (49-460)"
     misc_feature    2274..2318
                     /label=HT7 Linker C terminal
     misc_feature    2319..3209
                     /label=HT7 tag C terminal
     polyA_signal    3343..3564
                     /label=Sv40 late polyadenylation signal
     promoter        3663..4081
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4089..4155
                     /label=EM7 bacterial promoter
     CDS             4169..4963
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5027..5075
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5311..5347
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggag acctccaacc
     1081 ctaacaagcc caagaggcag accaaccaac tgcaatacct gctcagagtg gtgctcaaga
     1141 cactatggaa acaccagttt gcatggcctt tccagcagcc tgtggatgcc gtcaagctga
     1201 acctccctga ttactataag atcattaaaa cgcctatgga tatgggaaca ataaagaagc
     1261 gcttggaaaa caactattac tggaatgctc aggaatgtat ccaggacttc aacactatgt
     1321 ttacaaattg ttacatctac aacaagcctg gagatgacat agtcttaatg gcagaagctc
     1381 tggaaaagct cttcttgcaa aaaataaatg agctacccac agaagaaacc gagatcatga
     1441 tagtccaggc aaaaggaaga ggacgtggga ggaaagaaac agggacagca aaacctggcg
     1501 tttccacggt accaaacaca actcaagcat cgactcctcc gcagacccag acccctcagc
     1561 cgaatcctcc tcctgtgcag gccacgcctc accccttccc tgccgtcacc ccggacctca
     1621 tcgtccagac ccctgtcatg acagtggtgc ctccccagcc actgcagacg cccccgccag
     1681 tgccccccca gccacaaccc ccacccgctc cagctcccca gcccgtacag agccacccac
     1741 ccatcatcgc ggccacccca cagcctgtga agacaaagac caccattgac cccattcacg
     1801 agccaccctc gctgcccccg gagcccaaga ccaccaagct gggccagcgg cgggagagca
     1861 gccggcctgt gaaacctcca aagaaggacg tgcccgactc tcagcagcac ccagcaccag
     1921 agaagagcag caaggtctcg gagcagctca agtgctgcag cggcatcctc aaggagatgt
     1981 ttgccaagaa gcacgccgcc tacgcctggc ccttctacaa gcctgtggac gtggaggcac
     2041 tgggcctaca cgactactgt gacatcatca agcaccccat ggacatgagc acaatcaagt
     2101 ctaaactgga ggcccgtgag taccgtgatg ctcaggagtt tggtgctgac gtccgattga
     2161 tgttctccaa ctgctataag tacaaccctc ctgaccatga ggtggtggcc atggcccgca
     2221 agctccagga tgtgttcgaa atgcgctttg ccaagatgcc ggacgaggtt tctctcgagc
     2281 caaccactga ggatctgtac tttcagagcg ataacgatgg atccgaaatc ggtactggct
     2341 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
     2401 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
     2461 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
     2521 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
     2581 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
     2641 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
     2701 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
     2761 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
     2821 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
     2881 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
     2941 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
     3001 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     3061 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     3121 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     3181 gctggctgtc tactctggag atttccggtt aatagaattc tagagtcgac ctgcaggcat
     3241 gcaagctgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     3301 tgagcaataa ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca
     3361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     3421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     3481 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca
     3541 agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt
     3601 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat
     3661 ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     3721 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3781 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3841 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3901 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3961 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4021 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4081 taattaactg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     4141 gtgaggaact aaacccagga ggcagatcat gattgaacaa gatggattgc acgcaggttc
     4201 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4261 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4321 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     4381 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4441 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4501 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4561 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4621 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     4681 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     4741 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     4801 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     4861 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     4921 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     4981 gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt
     5041 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatcctctt
     5101 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     5161 ccgtttctgc ggactggctt tctacccggt atcagctcac tcaaaggcgg taatacggtt
     5221 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5281 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5341 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5521 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5641 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5701 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5761 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5821 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5881 gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5941 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6001 ctagatcctt ttatagtccg gaaatacagg aacgcacgct ggatggccct tcgctgggat
     6061 ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc
     6121 ctctggttgc ataggtattc atacggttaa aatttatcag gcgcgattgc ggcagttttt
     6181 cgggtggttt gttgccattt ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag
     6241 cggtgcgtac aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg
     6301 agataaaccg cagtactccg gccacgatgc gtccggcgta gaggatcgag atct
//
