LOCUS       Exported                6717 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1067..1957
                     /label=HaloTag
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    1958..1996
                     /label=HT7 Linker N terminal
     misc_feature    1997..2004
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2006..3682
                     /label=PRKAA1
                     /note="PRKAA1"
     misc_feature    3683..3690
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3842..4063
                     /label=Sv40 late polyadenylation signal
     promoter        4162..4580
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4588..4654
                     /label=EM7 bacterial promoter
     CDS             4668..5462
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5526..5574
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    5617..6539
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    5794
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgccatgcg cagactcagt tcctggagaa agatggcgac
     2041 agccgagaag cagaaacacg acgggcgggt gaagatcggc cactacattc tgggtgacac
     2101 gctgggggtc ggcaccttcg gcaaagtgaa ggttggcaaa catgaattga ctgggcataa
     2161 agtagctgtg aagatactca atcgacagaa gattcggagc cttgatgtgg taggaaaaat
     2221 ccgcagagaa attcagaacc tcaagctttt caggcatcct catataatta aactgtacca
     2281 ggtcatcagt acaccatctg atattttcat ggtgatggaa tatgtctcag gaggagagct
     2341 atttgattat atctgtaaga atggaaggct ggatgaaaaa gaaagtcggc gtctgttcca
     2401 acagatcctt tctggtgtgg attattgtca caggcatatg gtggtccata gagatttgaa
     2461 acctgaaaat gtcctgcttg atgcacacat gaatgcaaag atagctgatt ttggtctttc
     2521 aaacatgatg tcagatggtg aatttttaag aacaagttgt ggctcaccca actatgctgc
     2581 accagaagta atttcaggaa gattgtatgc aggcccagag gtagatatat ggagcagtgg
     2641 ggttattctc tatgctttat tatgtggaac ccttccattt gatgatgacc atgtgccaac
     2701 tctttttaag aagatatgtg atgggatctt ctatacccct caatatttaa atccttctgt
     2761 gattagcctt ttgaaacata tgctgcaggt ggatcccatg aagagggcca caatcaaaga
     2821 tatcagggaa catgaatggt tcaaacagga ccttccaaaa tatctctttc ctgaggatcc
     2881 atcatatagt tcaaccatga ttgatgatga agccttaaaa gaagtatgtg aaaagtttga
     2941 gtgctcagaa gaggaagttc tcagctgtct ttacaacaga aatcaccagg atcctttggc
     3001 agttgcctac catctcataa tagataacag gagaataatg aatgaagcca aagatttcta
     3061 tttggcgaca agcccacctg attcttttct tgatgatcat cacctgactc ggccccatcc
     3121 tgaaagagta ccattcttgg ttgctgaaac accaagggca cgccataccc ttgatgaatt
     3181 aaatccacag aaatccaaac accaaggtgt aaggaaagca aaatggcatt taggaattag
     3241 aagtcaaagt cgaccaaatg atattatggc agaagtatgt agagcaatca aacaattgga
     3301 ttatgaatgg aaggttgtaa acccatatta tttgcgtgta cgaaggaaga atcctgtgac
     3361 aagcacttac tccaaaatga gtctacagtt ataccaagtg gatagtagaa cttatctact
     3421 ggatttccgt agtattgatg atgaaattac agaagccaaa tcagggactg ctactccaca
     3481 gagatcggga tcagttagca actatcgatc ttgccaaagg agtgattcag atgctgaggc
     3541 tcaaggaaaa tcctcagaag tttctcttac ctcatctgtg acctcacttg actcttctcc
     3601 tgttgaccta actccaagac ctggaagtca cacaatagaa ttttttgaga tgtgtgcaaa
     3661 tctaattaaa attcttgcac aagtttaaac gaattcgggc tcggtacccg gggatcctct
     3721 agagtcgacc tgcaggcatg caagctgatc cggctgctaa caaagcccga aaggaagctg
     3781 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcg gccgcttcga
     3841 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3901 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3961 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     4021 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatcga attttaacaa
     4081 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4141 tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa
     4201 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     4261 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4321 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4381 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4441 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4501 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4561 taggcttttg caaaaagctt aattaactgt tgacaattaa tcatcggcat agtatatcgg
     4621 catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg attgaacaag
     4681 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4741 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4801 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     4861 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4921 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4981 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5041 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5101 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5161 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5221 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5281 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5341 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5401 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5461 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg
     5521 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     5581 tagcgataag gatccgctca tgcgatgata agctgtcaaa catgagaatt acaacttata
     5641 tcgtatgggg ctgacttcag gtgctacatt tgaagagata aattgcactg aaatctagaa
     5701 atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc
     5761 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac
     5821 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc
     5881 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg
     5941 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg
     6001 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
     6061 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
     6121 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
     6181 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
     6241 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca
     6301 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
     6361 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
     6421 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg
     6481 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag
     6541 cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcatgaga ttacgcgttt
     6601 tagcggtgcg tacaattaag ggattatggt aaatccactt actgtctgcc ctcgtagcca
     6661 tcgagataaa ccgcagtact ccggccacga tgcgtccggc gtagaggatc gagatct
//
