LOCUS       Exported                9591 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     CDS             1056..5916
                     /label=Complete ORF
                     /note="Data entered semi automatically by Ethan on 
                     7/18/2011"
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1060..5916
                     /label=SMARCA4 insert
                     /note="Data entered semi automatically by Ethan on 
                     7/18/2011"
     misc_feature    1606..1611
                     /label=EcoICR I
     misc_feature    2271..2276
                     /label=EcoICR I
     misc_feature    3822..3827
                     /label=EcoICR I
     misc_feature    4989..4994
                     /label=EcoICR I
     misc_feature    complement(5319..5325)
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    5466..5471
                     /label=EcoICR I
     misc_feature    5919..5933
                     /label=linker
                     /note="linker"
     misc_feature    5934..6443
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    6580..6801
                     /label=Sv40 late polyadenylation signal
     promoter        6900..7318
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        7326..7392
                     /label=EM7 bacterial promoter
     CDS             7406..8200
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    8264..8312
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      8548..8584
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgtcc actccagacc
     1081 cacccctggg cggaactcct cggccaggtc cttccccggg ccctggccct tcccctggag
     1141 ccatgctggg ccctagcccg ggtccctcgc cgggctccgc ccacagcatg atggggccca
     1201 gcccagggcc gccctcagca ggacacccca tccccaccca ggggcctgga gggtaccctc
     1261 aggacaacat gcaccagatg cacaagccca tggagtccat gcatgagaag ggcatgtcgg
     1321 acgacccgcg ctacaaccag atgaaaggaa tggggatgcg gtcagggggc catgctggga
     1381 tggggccccc gcccagcccc atggaccagc actcccaagg ttacccctcg cccctgggtg
     1441 gctctgagca tgcctctagt ccagttccag ccagtggccc gtcttcgggg ccccagatgt
     1501 cttccgggcc aggaggtgcc ccgctggatg gtgctgaccc ccaggccttg gggcagcaga
     1561 accggggccc aaccccattt aaccagaacc agctgcacca gctcagagct cagatcatgg
     1621 cctacaagat gctggccagg gggcagcccc tccccgacca cctgcagatg gcggtgcagg
     1681 gcaagcggcc gatgcccggg atgcagcagc agatgccaac gctacctcca ccctcggtgt
     1741 ccgcaacagg acccggccct ggccctggcc ctggccccgg cccgggtccc ggcccggcac
     1801 ctccaaatta cagcaggcct catggtatgg gagggcccaa catgcctccc ccaggaccct
     1861 cgggcgtgcc ccccgggatg ccaggccagc ctcctggagg gcctcccaag ccctggcctg
     1921 aaggacccat ggcgaatgct gctgccccca cgagcacccc tcagaagctg attcccccgc
     1981 agccaacggg ccgcccttcc cccgcgcccc ctgccgtccc acccgccgcc tcgcccgtga
     2041 tgccaccgca gacccagtcc cccgggcagc cggcccagcc cgcgcccatg gtgccactgc
     2101 accagaagca gagccgcatc acccccatcc agaagccgcg gggcctcgac cctgtggaga
     2161 tcctgcagga gcgcgagtac aggctgcagg ctcgcatcgc acaccgaatt caggaacttg
     2221 aaaaccttcc cgggtccctg gccggggatt tgcgaaccaa agcgaccatt gagctcaagg
     2281 ccctcaggct gctgaacttc cagaggcagc tgcgccagga ggtggtggtg tgcatgcgga
     2341 gggacacagc gctggagaca gccctcaatg ctaaggccta caagcgcagc aagcgccagt
     2401 ccctgcgcga ggcccgcatc actgagaagc tggagaagca gcagaagatc gagcaggagc
     2461 gcaagcgccg gcagaagcac caggaatacc tcaatagcat tctccagcat gccaaggatt
     2521 tcaaggaata tcacagatcc gtcacaggca aaatccagaa gctgaccaag gcagtggcca
     2581 cgtaccatgc caacacggag cgggagcaga agaaagagaa cgagcggatc gagaaggagc
     2641 gcatgcggag gctcatggct gaagatgagg aggggtaccg caagctcatc gaccagaaga
     2701 aggacaagcg cctggcctac ctcttgcagc agacagacga gtacgtggct aacctcacgg
     2761 agctggtgcg gcagcacaag gctgcccagg tcgccaagga gaaaaagaag aaaaagaaaa
     2821 agaagaaggc agaaaatgca gaaggacaga cgcctgccat tgggccggat ggcgagcctc
     2881 tggacgagac cagccagatg agcgacctcc cggtgaaggt gatccacgtg gagagtggga
     2941 agatcctcac aggcacagat gcccccaaag ccgggcagct ggaggcctgg ctcgagatga
     3001 acccggggta tgaagtagct ccgaggtctg atagtgaaga aagtggctca gaagaagagg
     3061 aagaggagga ggaggaagag cagccgcagg cagcacagcc tcccaccctg cccgtggagg
     3121 agaagaagaa gattccagat ccagacagcg atgacgtctc tgaggtggac gcgcggcaca
     3181 tcattgagaa tgccaagcaa gatgtcgatg atgaatatgg cgtgtcccag gcccttgcac
     3241 gtggcctgca gtcctactat gccgtggccc atgctgtcac tgagagagtg gacaagcagt
     3301 cagcgcttat ggtcaatggt gtcctcaaac agtaccagat caaaggtttg gagtggctgg
     3361 tgtccctgta caacaacaac ctgaacggca tcctggccga cgagatgggc ctggggaaga
     3421 ccatccagac catcgcgctc atcacgtacc tcatggagca caaacgcatc aatgggccct
     3481 tcctcatcat cgtgcctctc tcaacgctgt ccaactgggc gtacgagttt gacaagtggg
     3541 ccccctccgt ggtgaaggtg tcttacaagg gatccccagc agcaagacgg gcctttgtcc
     3601 cccagctccg gagtgggaag ttcaacgtct tgctgacgac gtacgagtac atcatcaaag
     3661 acaagcacat cctcgccaag atccgttgga agtacatgat tgtggacgaa ggtcaccgca
     3721 tgaagaacca ccactgcaag ctgacgcagg tgctcaacac gcactatgtg gcaccccgcc
     3781 gcctgctgct gacgggcaca ccgctgcaga acaagcttcc cgagctctgg gcgctgctca
     3841 acttcctgct gcccaccatc ttcaagagct gcagcacctt cgagcagtgg tttaacgcac
     3901 cctttgccat gaccggggaa aaggtggacc tgaatgagga ggaaaccatt ctcatcatcc
     3961 ggcgtctcca caaagtgctg cggcccttct tgctccgacg actcaagaag gaagtcgagg
     4021 cccagttgcc cgaaaaggtg gagtacgtca tcaagtgcga catgtctgcg ctgcagcgag
     4081 tgctctaccg ccacatgcag gccaagggcg tgctgctgac tgatggctcc gagaaggaca
     4141 agaagggcaa aggcggcacc aagaccctga tgaacaccat catgcagctg cggaagatct
     4201 gcaaccaccc ctacatgttc cagcacatcg aggagtcctt ttccgagcac ttggggttca
     4261 ctggcggcat tgtccaaggg ctggacctgt accgagcctc gggtaaattt gagcttcttg
     4321 atagaattct tcccaaactc cgagcaacca accacaaagt gctgctgttc tgccaaatga
     4381 cctccctcat gaccatcatg gaagattact ttgcgtatcg cggctttaaa tacctcaggc
     4441 ttgatggaac cacgaaggcg gaggaccggg gcatgctgct gaaaaccttc aacgagcccg
     4501 gctctgagta cttcatcttc ctgctcagca cccgggctgg ggggctcggc ctgaacctcc
     4561 agtcggcaga cactgtgatc atttttgaca gcgactggaa tcctcaccag gacctgcaag
     4621 cgcaggaccg agcccaccgc atcgggcagc agaacgaggt gcgtgtgctc cgcctctgca
     4681 ccgtcaacag cgtggaggag aagatcctag ctgcagccaa gtacaagctc aacgtggacc
     4741 agaaggtgat ccaggccggc atgttcgacc agaagtcctc cagccatgag cggcgcgcct
     4801 tcctgcaggc catcctggag cacgaggagc aggatgagga ggaagacgag gtgcccgacg
     4861 acgagaccgt caaccagatg atcgcccggc acgaggagga gtttgatctg ttcatgcgca
     4921 tggacctgga ccgcaggcgc gaggaggccc gcaaccccaa gcggaagccg cgcctcatgg
     4981 aggaggacga gctcccctcg tggatcatca aggacgatgc ggaggtggag cggctgacct
     5041 gtgaggagga ggaggagaag atgttcggcc gtggctcccg ccaccgcaag gaggtggact
     5101 acagcgactc actgacggag aagcagtggc tcaagaccct gaaggccatc gaggagggca
     5161 cgctggagga gatcgaagag gaggtccggc agaagaaatc atcacggaag cgcaagcgag
     5221 acagcgacgc cggctcctcc accccgacca ccagcacccg cagccgcgac aaggacgacg
     5281 agagcaagaa gcagaagaag cgcgggcggc cgcctgccga gaaactctcc cctaacccac
     5341 ccaacctcac caagaagatg aagaagattg tggatgccgt gatcaagtac aaggacagca
     5401 gtggacgtca gctcagcgag gtcttcatcc agctgccctc gcgaaaggag ctgcccgagt
     5461 actacgagct catccgcaag cccgtggact tcaagaagat aaaggagcgc attcgcaacc
     5521 acaagtaccg cagcctcaac gacctagaga aggacgtcat gctcctgtgc cagaacgcac
     5581 agaccttcaa cctggagggc tccctgatct atgaagactc catcgtcttg cagtcggtct
     5641 tcaccagcgt gcggcagaaa atcgagaagg aggatgacag tgaaggcgag gagagtgagg
     5701 aggaggaaga gggcgaggag gaaggctccg aatccgaatc tcggtccgtc aaagtgaaga
     5761 tcaagcttgg ccggaaggag aaggcacagg accggctgaa gggcggccgg cggcggccga
     5821 gccgagggtc ccgagccaag ccggtcgtga gtgacgatga cagtgaggag gaacaagagg
     5881 aggaccgctc aggaagtggc agcgaagaag acgtttctct cggctcgagc ggcgtcttca
     5941 cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc
     6001 ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc
     6061 aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt
     6121 atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc
     6181 ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg
     6241 ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg
     6301 gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc
     6361 tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc
     6421 ggctgtgcga acgcattctg gcgtaaggcc gcgactctag agtcgacctg caggcatgca
     6481 agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     6541 gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg
     6601 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6661 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6721 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt
     6781 aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc
     6841 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg
     6901 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     6961 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7021 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7081 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7141 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7201 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7261 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttaa
     7321 ttaactgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     7381 aggaactaaa cccaggaggc agatcatgat tgaacaagat ggattgcacg caggttctcc
     7441 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     7501 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     7561 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     7621 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     7681 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     7741 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     7801 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     7861 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     7921 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     7981 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     8041 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     8101 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     8161 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     8221 atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt
     8281 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tcctctttgc
     8341 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     8401 tttctgcgga ctggctttct acccggtatc agctcactca aaggcggtaa tacggttatc
     8461 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8521 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8581 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8641 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8701 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8761 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8821 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8881 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8941 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9001 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9061 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9121 cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9181 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9241 gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     9301 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     9361 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc agtttttcgg
     9421 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     9481 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     9541 taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc t
//
