LOCUS       Exported                6571 bp ds-DNA     linear   SYN 30-MAY-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1179
                     /label=EF1a
                     /note="EF1a"
     misc_recomb     1250..1544
                     /label=R4 aatB
                     /note="R4 aatB"
     CDS             1558..2352
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    2376..2423
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      complement(3105..3141)
                     /direction=LEFT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     CDS             complement(3310..3969)
                     /label=Chloramphenicol resistance
     promoter        complement(3983..4049)
                     /label=EM7 bacterial promoter
     promoter        complement(4067..4621)
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     misc_feature    4631..4676
                     /label=Minimal CMV Promoter 2
     misc_feature    complement(4631..4653)
                     /label=core
                     /note="core"
     misc_feature    4679..4695
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(4696..4722)
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    4736..5248
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    5249..5260
                     /label=linker
                     /note="linker"
     misc_feature    5261..5268
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    5270..6271
                     /label=WDR5 ORF
                     /note="WDR5 ORF"
     misc_feature    5617..5622
                     /label=EcoICR I
     misc_feature    6272..6275
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    6276..6279
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    6317..6366
                     /label=MCS 2
     misc_feature    6370..6557
                     /label=SV40\polyA\signal
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg caggcagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa agccgaattc tgcagatatc
     1201 catcacactg tcgagcatgc atctagtcca gtgtggtgga attcggcttc gtggggacgc
     1261 cgtacaggga cgtgcacctc tcccgctgca ccgcctccag cgtcgccgcc ggctcgaagg
     1321 acggggccgg gatgacgatg caggcggcgt gggaggtggc gcccaagttg cccatgacca
     1381 tgccgaagca gtggtagaag ggcaccggca gacacacccg gtcctgctcc gtgtagccga
     1441 ccgtgcggcc cacccagtag ccgttgttga ggatgttgtg gtgggagagc gtggcgccct
     1501 tggggaagcc ggtggtgccg gaggtgtact ggatgttgac cgggaatcga tgccaccatg
     1561 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1621 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1681 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     1741 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     1801 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     1861 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     1921 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     1981 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     2041 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     2101 gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     2161 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     2221 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     2281 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     2341 gagttcttct gaactctagc catcacgatg gccgcaataa aatatcttta ttttcattac
     2401 atctgtgtgt tggttttttg tgtgacgatt ccggactata aaaggatcta ggtgaagatc
     2461 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2521 gaccccgtag aaaagatcaa aggatcttct tgaaatcctt tttttctgcg cgtaatctgc
     2581 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2641 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2701 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2761 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2821 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2881 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2941 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3001 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3061 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3121 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3181 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3241 accgcctttg agtgagctga taccggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     3301 gagtcccgct tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     3361 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag
     3421 caccttgtcg ccttgcgtat aatatttgcc catagtgaaa acgggggcga agaagttgtc
     3481 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     3541 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     3601 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     3661 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     3721 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
     3781 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     3841 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     3901 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     3961 tttctccatg atctgcctcc tgggtttagt tcctcacctt gtcgtattat actatgccga
     4021 tatactatgc cgatgattaa ttgtcaacag tattagaatt cgatttgacc tcccaccgta
     4081 cacgcctacc gcccatttgc gtcaacgggg cggggttatt acgacatttt ggaaagtccc
     4141 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     4201 cccgtgagtc aaaccgctat ccacgcccat tggtgtactg ccaaaaccgc atcaccatgg
     4261 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccgt aaggtcatgt
     4321 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcggacttg
     4381 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     4441 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     4501 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     4561 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     4621 tggcgcgcca gggaggccta tataagcaga gctggtttag tgaaccgtca gatcgcccat
     4681 ttaggtgaca ctatagaata caagctactt gttctttttg cagctagcgc tcaccatggt
     4741 cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca acctggacca
     4801 agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc
     4861 gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat
     4921 cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt
     4981 gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac tggtaatcga
     5041 cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca tcgccgtgtt
     5101 cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa ttatcgacga
     5161 gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg gagtgaccgg
     5221 ctggcggctg tgcgaacgca ttctggcggg ctcgagcggc gcgatcgcca tggcgacgga
     5281 ggagaagaag cccgagaccg aggccgccag agcacagcca accccttcgt catccgccac
     5341 tcagagcaag cctacacctg tgaagccaaa ctatgctcta aagttcaccc ttgctggcca
     5401 caccaaagca gtgtcctccg tgaaattcag cccgaatgga gagtggctgg caagttcatc
     5461 tgctgataaa cttattaaaa tttggggcgc gtatgatggg aaatttgaga aaaccatatc
     5521 tggtcacaag ctgggaatat ccgatgtagc ctggtcgtca gattctaacc ttcttgtttc
     5581 tgcctcagat gacaaaacct tgaagatatg ggacgtgagc tcgggcaagt gtctgaaaac
     5641 cctgaaggga cacagtaatt atgtcttttg ctgcaacttc aatccccagt ccaaccttat
     5701 tgtctcagga tcctttgacg aaagcgtgag gatatgggat gtgaaaacag ggaagtgcct
     5761 caagactttg ccagctcact cggatccagt ctcggccgtt cattttaatc gtgatggatc
     5821 cttgatagtt tcaagtagct atgatggtct ctgtcgcatc tgggacaccg cctcaggcca
     5881 gtgcctgaag acgctcatcg atgacgacaa cccccccgtg tcttttgtga agttctcccc
     5941 gaacggcaaa tacatcctgg ccgccacgct ggacaacact ctgaagctct gggactacag
     6001 caaggggaag tgcctgaaga cgtacactgg ccacaagaat gagaaatact gcatatttgc
     6061 caatttctct gttactggtg ggaagtggat tgtgtctggc tcagaggata accttgttta
     6121 catctggaac cttcagacga aagagattgt acagaaacta caaggccaca cagatgtcgt
     6181 gatctcaaca gcttgtcacc caacagaaaa catcatcgcc tctgctgcgc tagaaaatga
     6241 caaaacaatt aaactgtgga agagtgactg cgtttaaacg aattcgggct cggtacccgg
     6301 ggatcctcta gaaacaccta ggctctcgta cgaagtcagc tgataacgtc gacgtattgc
     6361 ggccgcggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     6421 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6481 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6541 catttttttc actgccgacg tcacgcgtct t
//
