LOCUS       Exported                6217 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN21A(pF-47nA)-PSMD3      6307 bp    DNA   circular    
                   11/7/2016

            DEFINITION  Current Name: pFN21A (pF-47nA) - PSMD3 Old/Alternate 
            Name(s):P58 (insert) proteasome (prosome, macropain) 26S subunit, 
            non-ATPase, 3 (insert) PSMD3 (insert) RPN3 (insert) S3 (insert) 
            pF-47nA (backbone)
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1067..3523
                     /label=Complete ORF
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:34:01 AM"
     misc_feature    1067..1957
                     /label=HT7
     misc_feature    1958..1996
                     /label=HT7 Linker N terminal
     misc_feature    1970..1990
                     /label=TEV protease recognition sequence
     misc_feature    1997..2004
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2001..3521
                     /label=PSMD3 insert
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:34:01 AM"
     misc_feature    2006..3517
                     /label=YAP1
                     /note="YAP1"
     misc_feature    2376..2381
                     /label=EcoICR I
     misc_feature    2414..2420
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2655..2660
                     /label=6base
     misc_feature    complement(3204..3209)
                     /label=6base
     misc_feature    complement(3362..3367)
                     /label=6base
     misc_feature    3518..3525
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3522..3655
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    3677..3898
                     /label=Sv40 late polyadenylation signal
     CDS             4159..5019
                     /label=beta-lactamase
     rep_origin      5174..5210
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    5881..6166
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgccatgga tcccgggcag cagccgccgc ctcaaccggc
     2041 cccccagggc caagggcagc cgccttcgca gcccccgcag gggcagggcc cgccgtccgg
     2101 acccgggcaa ccggcacccg cggcgaccca ggcggcgccg caggcacccc ccgccgggca
     2161 tcagatcgtg cacgtccgcg gggactcgga gaccgacctg gaggcgctct tcaacgccgt
     2221 catgaacccc aagacggcca acgtgcccca gaccgtgccc atgaggctcc ggaagctgcc
     2281 cgactccttc ttcaagccgc cggagcccaa atcccactcc cgacaggcca gtactgatgc
     2341 aggcactgca ggagccctga ctccacagca tgttcgagct cattcctctc cagcttctct
     2401 gcagttggga gctgtttctc ctgggacact gacccccact ggagtagtct ctggcccagc
     2461 agctacaccc acagctcagc atcttcgaca gtcttctttt gagatacctg atgatgtacc
     2521 tctgccagca ggttgggaga tggcaaagac atcttctggt cagagatact tcttaaatca
     2581 catcgatcag acaacaacat ggcaggaccc caggaaggcc atgctgtccc agatgaacgt
     2641 cacagccccc accagtccac cagtgcagca gaatatgatg aactcggctt caggtcctct
     2701 tcctgatgga tgggaacaag ccatgactca ggatggagaa atttactata taaaccataa
     2761 gaacaagacc acctcttggc tagacccaag gcttgaccct cgttttgcca tgaaccagag
     2821 aatcagtcag agtgctccag tgaaacagcc accacccctg gctccccaga gcccacaggg
     2881 aggcgtcatg ggtggcagca actccaacca gcagcaacag atgcgactgc agcaactgca
     2941 gatggagaag gagaggctgc ggctgaaaca gcaagaactg cttcggcagg caatgcggaa
     3001 tatcaatccc agcacagcaa attctccaaa atgtcaggag ttagccctgc gtagccagtt
     3061 accaacactg gagcaggatg gtgggactca aaatccagtg tcttctcccg ggatgtctca
     3121 ggaattgaga acaatgacga ccaatagctc agatcctttc cttaacagtg gcacctatca
     3181 ctctcgagat gagagtacag acagtggact aagcatgagc agctacagtg tccctcgaac
     3241 cccagatgac ttcctgaaca gtgtggatga gatggataca ggtgatacta tcaaccaaag
     3301 caccctgccc tcacagcaga accgtttccc agactacctt gaagccattc ctgggacaaa
     3361 tgtggacctt ggaacactgg aaggagatgg aatgaacata gaaggagagg agctgatgcc
     3421 aagtctgcag gaagctttga gttctgacat ccttaatgac atggagtctg ttttggctgc
     3481 caccaagcta gataaagaaa gctttcttac atggttagtt taaacgaatt cgggctcggt
     3541 acccggggat cctctagagt cgacctgcag gcatgcaagc tgatccggct gctaacaaag
     3601 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     3661 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     3721 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3781 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3841 gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa atgtggtaaa
     3901 atcgaattct aatggatcct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
     3961 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt aatggtttct
     4021 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4141 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4201 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4261 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4321 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcactttcaa agttctgcta
     4381 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4441 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4501 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4561 ttacttctga caactatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4621 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4681 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4741 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4801 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4861 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4921 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4981 atcgctgaga taggtgcctc actgattaag cattggtaat tcgaaatgac cgaccaagcg
     5041 acgcccaacc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5101 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5161 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5221 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5281 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5341 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5401 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5461 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5521 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5581 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5641 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5701 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5761 ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5821 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt
     5881 ccggaaatac aggaacgcac gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa
     5941 tggcagcttc agtggattaa gtgggggtaa tgtggcctgt accctctggt tgcataggta
     6001 ttcatacggt taaaatttat caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca
     6061 tttttacctg tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag
     6121 ggattatggt aaatccactt actgtctgcc ctcgtagcca tcgagataaa ccgcagtact
     6181 ccggccacga tgcgtccggc gtagaggatc gagatct
//
