LOCUS       Exported                6006 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC27K(pFC27K)      5527 bp    DNA   circular           
            3/27/2014

            DEFINITION  Current Name: pFC27K (pFC27K) Old/Alternate 
            Name(s):pFC27K (backbone)
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     CDS             1065..1919
                     /label=CNOT7
     misc_feature    1926..1970
                     /label=HT7 Linker C terminal
     misc_feature    1971..2861
                     /label=HT7 tag C terminal
     polyA_signal    2995..3216
                     /label=Sv40 late polyadenylation signal
     promoter        3315..3733
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3741..3807
                     /label=EM7 bacterial promoter
     CDS             3821..4615
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4679..4727
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      4963..4999
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcca gcggcaactg
     1081 tagatcatag ccaaagaatt tgtgaagttt gggcttgcaa cttggatgaa gagatgaaga
     1141 aaattcgtca agttatccga aaatataatt acgttgctat ggacaccgag tttccaggtg
     1201 tggttgcaag acccattgga gaattcagga gcaatgctga ctatcaatac caactattgc
     1261 ggtgtaatgt agacttgtta aagataattc agctaggact gacatttatg aatgagcaag
     1321 gagaataccc tccaggaact tcaacttggc agtttaattt taaatttaat ttgacggagg
     1381 acatgtatgc ccaggactct atagagctac taacaacatc tggtatccag tttaaaaaac
     1441 atgaggagga aggaattgaa acccagtact ttgcagaact tcttatgact tctggagtgg
     1501 tcctctgtga aggggtcaaa tggttgtcat ttcatagcgg ttacgacttt ggctacttaa
     1561 tcaaaatcct aaccaactct aacttgcctg aagaagaact tgacttcttt gagatccttc
     1621 gattgttttt tcctgtcatt tatgatgtga agtacctcat gaagagctgc aaaaatctca
     1681 aaggtggatt acaggaggtg gcagaacagt tagagctgga acggatagga ccacaacatc
     1741 aggcaggatc tgattcattg ctcacaggaa tggccttttt caaaatgaga gaaatgttct
     1801 ttgaagatca tattgatgat gccaaatatt gtggtcattt gtatggcctt ggttctggtt
     1861 catcctatgt acagaatggc acagggaatg catatgaaga ggaagccaac aagcagtcag
     1921 tttctctcga gccaaccact gaggatctgt actttcagag cgataacgat ggatccgaaa
     1981 tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag cgcatgcact
     2041 acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt aacccgacct
     2101 cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc tgcattgctc
     2161 cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc ttcgacgacc
     2221 acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca
     2281 ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca
     2341 aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa tggccagaat
     2401 ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag ctgatcatcg
     2461 atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag
     2521 tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc
     2581 gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag
     2641 aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg ggcaccccag
     2701 gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct aactgcaagg
     2761 ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac ctgatcggca
     2821 gcgagatcgc gcgctggctg tctactctgg agatttccgg ttaatagaat tctagagtcg
     2881 acctgcaggc atgcaagctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     2941 tgctgccacc gctgagcaat aactagcata accccttggg gcggccgctt cgagcagaca
     3001 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3061 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3121 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     3181 tttttttaag caagtaaaac ctctacaaat gtggtaaaat cgaattttaa caaaatatta
     3241 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3301 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     3361 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     3421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3481 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3541 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3601 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3661 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3721 ttgcaaaaag cttaattaac tgttgacaat taatcatcgg catagtatat cggcatagta
     3781 taatacgaca aggtgaggaa ctaaacccag gaggcagatc atgattgaac aagatggatt
     3841 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3901 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3961 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4021 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4081 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4141 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4201 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4261 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4321 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     4381 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4441 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4501 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4561 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4621 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa
     4681 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat
     4741 aaggatcctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     4801 ccggggtcag caccgtttct gcggactggc tttctacccg gtatcagctc actcaaaggc
     4861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5401 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5521 gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg
     5581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5641 aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg ctggatggcc
     5701 cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag tgggggtaat
     5761 gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc aggcgcgatt
     5821 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg
     5881 aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc
     5941 tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg
     6001 agatct
//
