LOCUS       Exported                6358 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    complement(192..254)
                     /label=MCS 2
     misc_feature    192..199
                     /label=NotI
                     /note="NotI"
     misc_feature    197..218
                     /label=HnHc_dR4_1
                     /note="HnHc_dR4_1"
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     misc_feature    620..638
                     /label=HnHc_dR4_2
                     /note="HnHc_dR4_2"
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1170..2060
                     /label=HaloTag
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    complement(1210..1230)
                     /label=HTnR
                     /note="HTnR"
     misc_feature    1607..1625
                     /label=HnHc_dR4_3
                     /note="HnHc_dR4_3"
     misc_feature    2061..2099
                     /label=HT7 Linker N terminal
     misc_feature    2073..2093
                     /label=TEV protease recognition sequence
     misc_feature    2100..2107
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2109..3287
                     /label=p53
                     /note="Recieved from Kazusa in April 2010. Entered into 
                     database semi automatically by Ethan in May 2010."
     misc_feature    3288..3295
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3327..3342
                     /label=HnHc_dR4_4
                     /note="HnHc_dR4_4"
     misc_feature    3370..3371
                     /label=BlpI
                     /note="BlpI"
     polyA_signal    3391..3612
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(3636..3669)
                     /label=Cre site
                     /note="Cre site"
     promoter        3678..3744
                     /label=EM7 bacterial promoter
     CDS             3758..4552
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    4132..4149
                     /label=HnHc_dR4_5
                     /note="HnHc_dR4_5"
     rep_origin      4707..4743
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    4729..4751
                     /label=HnHc_dR4_6
                     /note="HnHc_dR4_6"
     misc_feature    5325..5343
                     /label=HnHc_dR4_7
                     /note="HnHc_dR4_7"
     polyA_signal    complement(5425..5472)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(5496..5894)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(5919..6337)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    5928..5945
                     /label=HnHc_dR4_8
                     /note="HnHc_dR4_8"
     misc_feature    join(6329..6358,1..3)
                     /label=RSGBFB15 (primer)
                     /note="RSGBFB15 (primer)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc aaagccacca tggcagaaat cggtactggc tttccattcg
     1201 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     1261 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     1321 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     1381 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     1441 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     1501 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     1561 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     1621 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     1681 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     1741 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     1801 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     1861 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     1921 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     1981 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     2041 cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag agcgataacg
     2101 cgatcgccat ggaggagccg cagtcagatc ctagcgtcga gccccctctg agtcaggaaa
     2161 cattttcaga cctatggaaa ctacttcctg aaaacaacgt tctgtccccc ttgccgtccc
     2221 aagcaatgga tgatttgatg ctgtccccgg acgatattga acaatggttc actgaagacc
     2281 caggtccaga tgaagctccc agaatgccag aggctgctcc ccccgtggcc cctgcaccag
     2341 cagctcctac accggcggcc cctgcaccag ccccctcctg gcccctgtca tcttctgtcc
     2401 cttcccagaa aacctaccag ggcagctacg gtttccgtct gggcttcttg cattctggga
     2461 cagccaagtc tgtgacttgc acgtactccc ctgccctcaa caagatgttt tgccaactgg
     2521 ccaagacctg ccctgtgcag ctgtgggttg attccacacc cccgcccggc acccgcgtcc
     2581 gcgccatggc catctacaag cagtcacagc acatgacgga ggttgtgagg cgctgccccc
     2641 accatgagcg ctgctcagat agcgatggtc tggcccctcc tcagcatctt atccgagtgg
     2701 aaggaaattt gcgtgtggag tatttggatg acagaaacac ttttcgacat agtgtggtgg
     2761 tgccctatga gccgcctgag gttggctctg actgtaccac catccactac aactacatgt
     2821 gtaacagttc ctgcatgggc ggcatgaacc ggaggcccat cctcaccatc atcacactgg
     2881 aagactccag tggtaatcta ctgggacgga acagctttga ggtgcgtgtt tgtgcctgtc
     2941 ctgggagaga ccggcgcaca gaggaagaga atctccgcaa gaaaggggag cctcaccacg
     3001 agctgccccc agggagcact aagcgagcac tgcccaacaa caccagctcc tctccccagc
     3061 caaagaagaa accactggat ggagaatatt tcacccttca gatccgtggg cgtgagcgct
     3121 tcgagatgtt ccgagagctg aatgaggcct tggaactcaa ggatgcccag gctgggaagg
     3181 agccaggggg gagcagggct cactccagcc acctgaagtc caaaaagggt cagtctacct
     3241 cccgccataa aaaactcatg ttcaagacag aagggcctga ctcagacgtt taaacgaatt
     3301 cgggctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc tgatttggct
     3361 gctgccaccg ctgagcaagg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     3421 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3481 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3541 attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag taaaacctct
     3601 acaaatgtgg taaaatctta attaagaatt ctaatataac ttcgtatagc atacattata
     3661 cgaagttatg gatccactgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     3721 tacgacaagg tgaggaacta aacccaggag gcagatcatg attgaacaag atggattgca
     3781 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     3841 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     3901 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     3961 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4021 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4081 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4141 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4201 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4261 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     4321 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4381 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4441 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4501 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gattcgaaat
     4561 gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat acggttatcc
     4621 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     5161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5281 agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5401 atccttttat agtccggaat cgtcacacaa aaaaccaaca cacagatgta atgaaaataa
     5461 agatatttta ttgcggccat cgtgatggct agagtttagc cctcccacac ataaccagag
     5521 ggcagcaatt cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatggct
     5581 ttgatcccag gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg
     5641 cccctgttct catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca
     5701 gcagtgccca gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca
     5761 ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag
     5821 atggggatgc tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac
     5881 aaaggcttgg ccatggtggc gctagtgtca gaagaatcga gctttttgca aaagcctagg
     5941 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     6001 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     6061 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     6121 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     6181 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     6241 cctaactgac acacattcca cagctggttc tttccgcctc agaaggtacc taaccaagtt
     6301 cctctttcag aggttatttc aggccatggt gctgcgcaag acgcgttaat taagacgt
//
