LOCUS       Exported                6821 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    805..2086
                     /label=TEAD1 NM021961
                     /note="TEAD1 NM021961"
     misc_feature    complement(2088..2095)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2100..2126
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2127..2143)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2146..2191)
                     /label=Minimal CMV Promoter 2
     misc_feature    2169..2191
                     /label=core
                     /note="core"
     promoter        2201..2755
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2773..2839
                     /label=EM7 bacterial promoter
     CDS             2853..3512
                     /label=Chloramphenicol resistance
     rep_origin      3681..3717
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4399..4446)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4470..5264)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5278..5572)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5643..6821)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacgt cctttacaag cctgtaaata tgatgttgtg
      841 ctccgtgttc actatttgaa acttcaaaca cacaggccat gcagagtaga gtttcttgtg
      901 tatccctgtt tgttaccacc aataaaattg tgaagttttc caaaacactg ttcatcatat
      961 atttctctgg taagtgtttg agcttgtgga tgaagttgat catatattca cacattgggg
     1021 agcggtttat tcggtataca aatcggccat tctcaaacct tgcatactcc gtctctactt
     1081 tttctactac ttgcttccca aaggagcaaa ctttggtgga acaggtgact gtcatatttt
     1141 cagaactctc gtactgactg gttacaccat aaaaagcccc agcatcatct tgaatattgc
     1201 agtttaaatc agcccagaat tttacgagga agaaggcatt ttgagggccc tttccaaaca
     1261 gttcctttaa gccacctttc ttttcaggaa atttgtcata aatctgacga atgtccactg
     1321 attcaagcaa tgggtcactg taagaatggt tggcatgccc aatgtgcacg aagaggtgtt
     1381 tgttgtacga gtctgggtct cgctgctgct cgagaaaagc tgaaaattcc accaggcgaa
     1441 gcttggttgt gccaatggag cgaccttgcc aggcagggac tgagggagct ggggccgatg
     1501 caggctcaaa ccctggaatg ggggctgtga ccgctggctg gatggggtag gcctgctgca
     1561 caaaaggctt gacgtcttgt gaggatcctg gctgccctgt ttgaatcatt cccggccaga
     1621 accccggcgc ccctgggaag gtcgggcgtg gaatcccagg cagccccagc ttgttatgaa
     1681 tggcagtggc cgagacgatc tgggctgagg acatggccgc catgtgctgc agggccttat
     1741 cctttgcagt ctgatccttt agcttggaat gaaaatcacg agatttcctt ctggcaagaa
     1801 cctgaatgtg actagacacc tgttttctgg tcctcgtctt gcctgtcctg agtttgatgt
     1861 atctggctat caattcattc ctaccataca ttttgccttc gtctgataag atgattttcc
     1921 tcctcccaca tggtggatag atagccaggg cctcctgaaa gctttgctcg atgtcggggc
     1981 tccagacccc ttctgcatca ttgtcaattg gcttatctgc agagtcactc atcctttcca
     2041 tgttttcggc agggctctca ctgccgctcc agctgctggg ctccatggcg atcgctagct
     2101 gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatgggcgat ctgacggttc
     2161 actaaaccag ctctgcttat ataggcctcc ctggcgcgcc actagttatt aatagtaatc
     2221 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2281 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2341 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2401 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     2461 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     2521 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2581 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2641 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2701 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtcaaatc
     2761 gaattctaat actgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     2821 caaggtgagg aactaaaccc aggaggcaga tcatggagaa aaaaatcact ggatatacca
     2881 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     2941 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     3001 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     3061 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     3121 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     3181 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     3241 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     3301 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     3361 ttttcactat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     3421 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     3481 agtactgcga tgagtggcag ggcggggcgt aagcgggact ctggggttcg aaatgaccga
     3541 ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4261 aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4381 ttatagtccg gaatcgtcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat
     4441 tttattgcgg ccatcgtgat ggctagagtt cagaagaact cgtcaagaag gcgatagaag
     4501 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     4561 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     4621 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     4681 ttcggcaagc aggcatcgcc gtgggtcacg acgagatcct cgccgtcggg catgcgcgcc
     4741 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     4801 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     4861 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     4921 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     4981 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     5041 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     5101 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     5161 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     5221 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatggtggc atcgattccc
     5281 ggtcaacatc cagtacacct ccggcaccac cggcttcccc aagggcgcca cgctctccca
     5341 ccacaacatc ctcaacaacg gctactgggt gggccgcacg gtcggctaca cggagcagga
     5401 ccgggtgtgt ctgccggtgc ccttctacca ctgcttcggc atggtcatgg gcaacttggg
     5461 cgccacctcc cacgccgcct gcatcgtcat cccggccccg tccttcgagc cggcggcgac
     5521 gctggaggcg gtgcagcggg agaggtgcac gtccctgtac ggcgtcccca cgaagccgaa
     5581 ttccaccaca ctggactaga tgcatgctcg acagtgtgat ggatatctgc agaattcggc
     5641 tttcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg
     5701 aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct
     5761 ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc
     5821 ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc
     5881 gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt
     5941 tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctgcct
     6001 gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc
     6061 cttgccgccc agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg
     6121 ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc
     6181 gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata
     6241 ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca
     6301 gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga
     6361 aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca
     6421 cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga
     6481 agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg
     6541 ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg
     6601 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     6661 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     6721 cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact
     6781 gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc c
//
