LOCUS       Exported                7886 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     misc_feature    complement(805..808)
                     /label=PmeI
                     /note="PmeI"
     misc_feature    complement(809..3151)
                     /label=B-catenin
                     /note="B-catenin"
     misc_feature    complement(3153..3155)
                     /label=SgfI
                     /note="SgfI"
     misc_feature    complement(3156..3160)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3165..3191
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(3192..3208)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(3211..3256)
                     /label=Minimal CMV Promoter 2
     misc_feature    3234..3256
                     /label=core
                     /note="core"
     promoter        3266..3820
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        3838..3904
                     /label=EM7 bacterial promoter
     CDS             3918..4577
                     /label=Chloramphenicol resistance
     rep_origin      4746..4782
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5464..5511)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(5535..6329)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(6343..6637)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(6708..7886)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacca ggtcagtatc aaaccaggcc agctgattgc
      841 tgtcacctgg aggcagccca tccatgaggt cctgggcatg ccccagatct ggcagcccat
      901 caactggata gtcagcacca gggtggtggc cacccatctc atgttccatc atggggtcca
      961 tacccaaggc atcctggcca tatccaccag agtgaaaaga acgatagcta ggatcatcct
     1021 ggcgatatcc aaggggttct ccctgggcac caatatcaag tccaagatca gcagtctcat
     1081 tccaagccat tggctctgtt ctgaagagag agctggtcag ctcaactgaa agccgtttct
     1141 tgtaatcttg tggcttgtcc tcagacattc ggaacaaaac agcagctgca tatgtcgcca
     1201 caccttcatt cctagagtga agtaactctg tcagaggagc tgtggctccc tcagcttcaa
     1261 tagcttctgc agcttccttg tcctgagcaa gttcacagag gacccctgca gctactcttt
     1321 ggatgttttc aatgggagaa taaagcagct gcacaaacaa tggaatggta tttagtcctc
     1381 tgataacaat tcggttgtga acatcccgag ctaggatgtg aagggctccg gtacaacctt
     1441 caactatttc ttccatgcgg accccctcca caaattgctg ctgtgtccca cccatggacg
     1501 tacggcgctg ggtatcctga tgtgcacgaa caagcaactg aactagtcgt ggaatggcac
     1561 cctgctcacg caaaggtgca tgatttgcgg gacaaagggc aagatttcga atcaatccaa
     1621 cagtagcctt tatcagaggc cagtgggatg gtgggtgtaa gagcttaacc acaactggta
     1681 gtccatagtg aaggcgaact gcattctggg ccatctctgc ttcttggtgt cggctggtca
     1741 gatgacgaag agcacagatg gcaggctcag tgatgtcttc cctgtcacca gcccgaagga
     1801 cagtacgcac aagagcctct ataccaccca cttggcagac catcatcttg ttcttataat
     1861 tattgcaagt gaggttagaa agaattccag ctgcacaggt gaccacattt atatcatctg
     1921 aacccagaag ctgaacaaga gtcccaagga gaccttccat cccttcctgt ttagttgcag
     1981 catctgaaag attcctgaga gtccaaagac agttctgaac aagacgttga cttggatctg
     2041 tcaggtgaag tcctaaagct tgcattccac cagcttctac aatagccggc ttattactag
     2101 agcagacaga tagcaccttc agcactctgc ttgtggtcca cagtagtttt tcgtaagtat
     2161 aggtcctcat tatatttact aaagcttggg gtccaccact agccagtatg atgagcttgc
     2221 tttcttggtt gccataagct aaaatttgaa ggcagtctgt cgtaatagcc aagaatttaa
     2281 catttgtttt gttgagcaag gcaaccattt tctgcagccc accagctaaa cgcactgcca
     2341 ttttagctcc ttcttgatgt aataaaaggt tgtggagagt tgtaatggca taaaacaaca
     2401 cagaatccac tggtgaacca agcattttca ccagggcagg aatgcctcca gacttaaaga
     2461 tggccagtaa gccctcacga tgatgggaaa ggttatgcaa ggtcccagcg gtacaacgag
     2521 ctgtttctac atcatttgta ttctgcatgg tacgtacaat agcagacacc atctgaggag
     2581 aacgcatgat agcgtgtctg gaagcttcct ttttagaaag ctgatggacc ataactgcag
     2641 ccttattaac caccacctgg tcctcgtcat ttagcagttt tgtcagttca gggattgcac
     2701 gtgtggcaag ttctgcatca tcttgatagt taatcaagtt tacaactgca tgtttcagca
     2761 tctgtgatgg ttcagccaaa cgctggacat tagtgggatg agcagcatca aactgtgtag
     2821 atgggatctg catgccctca tctaatgtct cagggaacat agcagctcgt accctctgag
     2881 ctcgagtcat tgcatactgt ccatcaatat cagctacttg ttcttgagtg aaggactgag
     2941 aaaatccctg ttcccactca tacaggactt gggaggtatc cacatcctct tcctcaggat
     3001 tgcctttacc actcagagaa ggagctgtgg tagtggcacc agaatggatt ccagagtcca
     3061 ggtaagactg ttgctgccag tgactaacag ccgcttttct gtctggttcc atggccatgt
     3121 ccaactccat caaatcagct tgagtagcca tggcgatcgc tagctgcaaa aagaacaagt
     3181 agcttgtatt ctatagtgtc acctaaatgg gcgatctgac ggttcactaa accagctctg
     3241 cttatatagg cctccctggc gcgccactag ttattaatag taatcaatta cggggtcatt
     3301 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     3361 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3421 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     3481 ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa
     3541 atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta
     3601 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg
     3661 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     3721 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc
     3781 gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc aaatcgaatt ctaatactgt
     3841 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     3901 aacccaggag gcagatcatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     3961 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     4021 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     4081 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     4141 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     4201 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     4261 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     4321 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     4381 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc actatgggca
     4441 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     4501 tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     4561 ggcagggcgg ggcgtaagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4621 ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4681 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4741 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4801 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4861 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4921 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4981 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5041 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5101 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5161 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     5221 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5281 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atttcaagaa
     5341 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5401 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata gtccggaatc
     5461 gtcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat tgcggccatc
     5521 gtgatggcta gagttcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     5581 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     5641 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     5701 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     5761 tcgccgtggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     5821 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     5881 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     5941 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     6001 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     6061 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     6121 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     6181 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     6241 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     6301 cctgcgtgca atccatcttg ttcaatcatg gtggcatcga ttcccggtca acatccagta
     6361 cacctccggc accaccggct tccccaaggg cgccacgctc tcccaccaca acatcctcaa
     6421 caacggctac tgggtgggcc gcacggtcgg ctacacggag caggaccggg tgtgtctgcc
     6481 ggtgcccttc taccactgct tcggcatggt catgggcaac ttgggcgcca cctcccacgc
     6541 cgcctgcatc gtcatcccgg ccccgtcctt cgagccggcg gcgacgctgg aggcggtgca
     6601 gcgggagagg tgcacgtccc tgtacggcgt ccccacgaag ccgaattcca ccacactgga
     6661 ctagatgcat gctcgacagt gtgatggata tctgcagaat tcggctttca cgacacctga
     6721 aatggaagaa aaaaactttg aaccactgtc tgaggcttga gaatgaacca agatccaaac
     6781 tcaaaaaggg caaattccaa ggagaattac atcaagtgcc aagctggcct aacttcagtc
     6841 tccacccact cagtgtgggg aaactccatc gcataaaacc cctcccccca acctaaagac
     6901 gacgtactcc aaaagctcga gaactaatcg aggtgcctgg acggcgcccg gtactccgtg
     6961 gagtcacatg aagcgacggc tgaggacgga aaggcccttt tcctttgtgt gggtgactca
     7021 cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc tgcctgcagc agggccggga
     7081 agcggccatc tttccgctca cgcaactggt gccgaccggg ccagccttgc cgcccagggc
     7141 ggggcgatac acggcggcgc gaggccaggc accagagcag gccggccagc ttgagactac
     7201 ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg
     7261 gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct
     7321 tggcccgcat ttacaagact atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa
     7381 ttttaaatgg ctagagactt atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt
     7441 cgcacgcggc ggccccagcg cccaggccag gcctcaactc aagcacgagg cgaaggggct
     7501 ccttaagcgc aaggcctcga actctcccac ccacttccaa cccgaagctc gggatcaaga
     7561 atcacgtact gcagccaggt ggaagtaatt caaggcacgc aagggccata acccgtaaag
     7621 aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc aaacccgttg
     7681 cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc accctcggga
     7741 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
     7801 gcaccggttc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
     7861 tctgcccact gacgggcacc ggagcc
//
