LOCUS       Exported               10484 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)-BAZ2A      10484 bp    DNA   circular  
                     2/2/2022

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) - BAZ2A Old/Alternate 
            Name(s):AB527356 (insert) BAZ2A (insert) bromodomain adjacent to 
            zinc finger domain protein (insert) bromodomain adjacent to zinc 
            finger domain, 2A (insert) bromodomain adjacent to zinc finger 
            domain, 2A, tr (insert) FHC10761 (insert) FXC10761 (insert) 
            pf1ke0092 (insert) TIP5 (insert) transcription termination factor 
            I-interacting pro (insert) TTF-I interacting peptide 5 (insert) 
            TTF-I-interacting protein 5 (insert) WALp3 (insert) pF-Nn5K3 
            (backbone)
FEATURES             Location/Qualifiers
     source          1..10484
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..7319
                     /label=Complete ORF
                     /note="InsertComparisonToAccessionNumber updated to 
                     Idenitcal to the CDS from AB527356 by Ethan Strauss on 
                     12/18/2012. InsertPrimerInformation updated to Unknown by 
                     Ethan Strauss on 12/18/2012."
     CDS             1065..1577
                     /label=N terminal NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1594..7317
                     /label=BAZ2A insert
                     /note="InsertComparisonToAccessionNumber updated to 
                     Idenitcal to the CDS from AB527356 by Ethan Strauss on 
                     12/18/2012. InsertPrimerInformation updated to Unknown by 
                     Ethan Strauss on 12/18/2012."
     misc_feature    2919..2925
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    4539..4544
                     /label=EcoICR I
     misc_feature    7314..7321
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    7318..7451
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    7473..7694
                     /label=Sv40 late polyadenylation signal
     promoter        7793..8211
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(7866..8102)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      8109..8174
                     /label=Sv40 Min Ori
     promoter        8219..8285
                     /label=EM7 bacterial promoter
     CDS             8299..9093
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    9157..9205
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      9441..9477
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    10148..10433
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggaaatggag gcaaacgacc
     1621 attttaactt tactggcctt ccccctgcac ctgctgcctc aggactgaaa ccctctcctt
     1681 cctcagggga gggcctctac actaacgggt ctcccatgaa cttcccccag caagggaaaa
     1741 gtttgaatgg ggatgtgaat gttaatggct tatctactgt atctcacact actacttcag
     1801 ggattttgaa ctctgctccc cactcctcca gcacctcaca cctccatcac cccagcgtgg
     1861 cctacgactg tctctggaac tactcacagt acccatctgc caatcctggc agcaacctca
     1921 aggacccacc ccttctctcc cagttctcgg ggggacaata cccactcaac ggcatccttg
     1981 ggggcagccg gcaaccttca tccccaagtc ataacactaa ccttcgggct gggagccaag
     2041 agttctgggc caacggtacc cagagtccca tggggcttaa ctttgattca caagaactgt
     2101 atgattcctt tcctgaccag aattttgagg tgatgcccaa tggaccccct agttttttca
     2161 cctccccaca gacttctcct atgttgggat ctagcattca aacctttgca ccctcccagg
     2221 aggtaggcag tggtatccat cctgatgagg cagcagaaaa ggagatgact tcagttgtgg
     2281 cagagaatgg cactggcttg gtaggcagct tggagctgga agaagagcag ccagaactga
     2341 agatgtgtgg ctacaatggc tctgtccctt ctgtggaatc gttacaccaa gaggtctcag
     2401 tcctggtccc tgaccccaca gtgagctgtt tagatgatcc ttcacatctt cctgatcaac
     2461 tggaagacac tccaatcctc agtgaagact ctctggagcc cttcaactct ctggcaccag
     2521 agccagtgag tggaggacta tatggtattg atgacacgga gctgatgggt gcagaggaca
     2581 agctgcctct tgaggacagc cctgtgattt ctgcccttga ttgcccttcc ctcaataatg
     2641 ctactgcctt cagtctcctg gcagatgata gtcaaacatc aacctctatc tttgccagtc
     2701 ccacctctcc acctgtccta ggggagtctg tcctgcaaga taacagcttt gacctgaata
     2761 atggtagtga cgctgaacag gaagaaatgg aaactcaatc ttcagacttc ccaccatccc
     2821 tgacccagcc agctcctgat cagtcatcca ctattcagct acatccagca acctcaccag
     2881 cagtctcgcc aacaacctcc ccagcagtct ccctagtggt ttctccagca gcctccccag
     2941 aaatctctcc agaagtttgt cccgcagctt ctacagttgt ctctccagca gtcttctcag
     3001 tggtctctcc agcttcctca gcagtcctcc cagcagtctc cttagaagtc ccgttgacgg
     3061 cttcagtgac atccccaaaa gcctctcccg taacttcccc agcagctgcc tttccaacag
     3121 cctccccagc aaataaggat gtcagcagct ttctagaaac cactgctgac gtggaagaga
     3181 tcactggaga aggactcact gcttctggta gtggtgatgt catgaggaga cgtattgcta
     3241 ccccagaaga agttcgtctt cccctccaac atgggtggcg gagagaggtg cgcatcaaga
     3301 agggcagcca ccgatggcag ggggagacct ggtattatgg cccctgtggg aagaggatga
     3361 agcaatttcc agaagtgatc aagtacctga gccgcaacgt ggtacacagt gtccgccgag
     3421 agcacttcag cttcagtccc cgtatgcctg ttggagattt ctttgaagaa agagacacgc
     3481 cagagggctt gcagtgggtg cagctctcag cagaggagat cccgtcgagg attcaggcaa
     3541 ttactggcaa acggggtcga cctcgaaaca ctgagaaggc taagactaag gaagtcccca
     3601 aggtgaaacg gggtcgaggt cggccaccta aggtcaaaat cactgagcta ttgaacaaga
     3661 cagacaaccg ccccctaaag aaactggagg cccaagaaac attgaatgag gaggataaag
     3721 caaagattgc taaaagcaag aagaagatga ggcagaaggt tcaacgggga gagtgtcaga
     3781 ctactatcca agggcaggcc agaaataagc ggaaacaaga gaccaagagc ttaaagcaga
     3841 aggaagctaa gaagaaatcc aaggctgaga aagaaaaagg aaagacaaag caggaaaaac
     3901 tgaaggaaaa agtcaagagg gaaaagaagg agaaggtaaa aatgaaggaa aaggaggagg
     3961 tgaccaaagc caagccagcc tgtaaagcag ataagaccct ggccacacag aggcgcttgg
     4021 aggaacggca gaggcagcag atgatcttgg aggaaatgaa gaagccgaca gaggatatgt
     4081 gtctgactga ccaccagccc ctgcctgact tctcacgagt ccctggtctg acattgccca
     4141 gtggagcctt ctcagactgc ttgaccattg tggagttcct gcatagcttt ggcaaggtgc
     4201 tgggctttga tcctgccaaa gatgtgccta gcctgggggt cctgcaggag ggactcctgt
     4261 gtcaaggtga cagcttgggt gaggtgcaag acctgctggt caggctgctg aaggctgcac
     4321 tccatgatcc tggctttccc tcctactgtc agtccctaaa gatcttgggg gagaaggtgt
     4381 ctgagatccc actgacaaga gacaatgtgt cagagatcct gcgctgcttc cttatggcat
     4441 atggagtaga gccagccctc tgtgaccgcc tgcgcaccca gccttttcag gcccagccac
     4501 cccagcagaa ggctgctgtc ctggccttcc ttgtgcatga gctcaatggc tccaccctca
     4561 tcatcaatga gattgacaag actctggaga gtatgtccag ctacaggaaa aacaagtgga
     4621 ttgttgaagg ccggctccgg aggctgaaaa ctgttctggc caagcgaact gggcggtctg
     4681 aagtagagat ggaagggcca gaggaatgcc tgggacggag gcgcagttct cggatcatgg
     4741 aggagaccag tggcatggaa gaagaggaag aagaggagtc tatagcagct gtccctggcc
     4801 gcaggggtcg aagagatgga gaggttgatg ccacagcatc tagcatccca gagctagagc
     4861 gccagataga aaaactcagc aagcgtcagc ttttctttcg caaaaagctg cttcactcat
     4921 cccagatgct tcgggcggtc tccctgggtc aggaccgcta cagacgtcgc tactgggtat
     4981 tgccgtattt ggctggtatc tttgtagaag gaacagaggg gaacttagtt cctgaggagg
     5041 tgataaagaa ggaaactgac tccttaaaag tggcagccca tgcgtcactc aaccctgccc
     5101 tcttctctat gaagatggag ttagctggct ccaacaccac tgccagttct cctgcccggg
     5161 cccgaggccg acctcgaaaa actaagcccg ggtctatgca acctaggcat cttaagtccc
     5221 ctgtcagggg tcaggattca gaacagcccc aggcccagct tcagcctgag gctcagcttc
     5281 atgctcctgc ccagccccag cctcagcttc agcttcagct tcagtcccat aaggggttcc
     5341 tggagcaaga aggctcccct ttgtcactgg gtcagagcca gcatgacctc agccagtcag
     5401 ccttcctgtc ttggctgagc cagactcaga gccatagctc cctgttgagc agctcagtcc
     5461 tcacacctga tagcagtccg ggaaaactag acccagctcc atcacaaccc ccggaggagc
     5521 cagagcctga tgaggcagaa tccagccctg atcctcaagc actctggttt aacatctcag
     5581 cccagatgcc ctgcaatgct gcccccacac cgccccctgc agtttctgag gaccaaccca
     5641 ctccctcccc tcagcagctt gcctcctcca agccaatgaa tagacctagt gctgccaacc
     5701 cttgttctcc agtgcagttc tcttccacgc ccttggctgg gttggcccct aagaggcgag
     5761 caggagaccc tggagaaatg ccacagagtc ccacagggct gggacagccc aaacggagag
     5821 ggagacctcc cagtaagttc ttcaaacaga tggaacagcg ttacctaacc cagctgacag
     5881 cccagcctgt cccacctgag atgtgctcag gctggtggtg gatacgagat cctgagatgt
     5941 tggatgccat gctcaaggcc ctacaccccc gaggtatccg ggagaaggca cttcacaaac
     6001 accttaacaa gcacagggac ttcttgcagg aagtctgcct gcggccctca gctgacccca
     6061 tctttgagcc caggcaacta cctgcctttc aagaagggat tatgagctgg tcccccaaag
     6121 agaagacata cgagacagac ctagcagtgc ttcaatgggt agaggagctg gagcagcggg
     6181 ttatcatgtc tgatctgcag attcggggct ggacatgtcc tagcccagac tctacccgtg
     6241 aagacttggc ctactgtgag cacctctccg actcccagga ggatatcacc tggcgaggtc
     6301 ggggcaggga gggactggca cctcagcgta aaactaccaa ccctttggac ctggctgtga
     6361 tgcggctggc tgccctggaa cagaatgtag aacggcggta cctgcgggag cccctctggc
     6421 caactcatga ggttgtgctg gagaaggccc tgcttagcac acctaatggt gcccctgagg
     6481 gcaccactac agagatatca tatgagatca cccctcgcat tcgtgtctgg cgccagaccc
     6541 tcgagcggtg ccggagcgca gcccaggtgt gcttgtgcct gggccagctg gagaggtcca
     6601 ttgcctggga gaagtctgtc aacaaagtga catgtctagt ctgccggaag ggtgacaatg
     6661 atgagtttct tctgctttgt gatgggtgtg accgtggctg ccacatttac tgccatcgtc
     6721 ccaagatgga ggctgtccca gaaggagatt ggttctgtac tgtctgtttg gctcagcagg
     6781 tggagggaga attcactcag aagcctggtt tcccaaagcg tggccagaag cggaaaagtg
     6841 gttattcgct gaacttctca gagggtgatg gccgccgacg ccgggtactg ttgaggggcc
     6901 gagaaagccc agcagcaggg cctcggtact cggaagaagg gctctccccc tccaagcggc
     6961 ggcgactctc tatgcggaac caccacagtg atctcacatt ttgcgagatt atcctgatgg
     7021 agatggagtc ccatgatgca gcctggcctt tcctagagcc tgtgaaccca cgtttggtga
     7081 gtgggtaccg gcgcatcatc aaaaatccta tggatttttc caccatgcgg gagcggctgc
     7141 tcaggggagg gtacaccagc tcagaggagt ttgcggctga tgccctcctg gtatttgaca
     7201 actgccagac tttcaacgag gatgactctg aagtaggcaa ggctgggcac atcatgcgcc
     7261 gcttcttcga gagccgctgg gaggagtttt atcagggaaa acaggccaat ctggtttaaa
     7321 cgaattcggg ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat
     7381 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     7441 ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     7501 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7561 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     7621 tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct
     7681 ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     7741 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     7801 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     7861 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7921 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8041 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8101 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8161 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taattaactg
     8221 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     8281 aaacccagga ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     8341 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     8401 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8461 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     8521 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8581 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8641 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8701 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8761 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     8821 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8881 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     8941 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     9001 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     9061 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     9121 accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta
     9181 catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatcctctt tgcgcttgcg
     9241 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
     9301 ggactggctt tctacccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9361 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     9901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10021 aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10141 ttatagtccg gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca
    10201 tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc
    10261 ataggtattc atacggttaa aatttatcag gcgcgattgc ggcagttttt cgggtggttt
    10321 gttgccattt ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac
    10381 aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg
    10441 cagtactccg gccacgatgc gtccggcgta gaggatcgag atct
//
