LOCUS       Exported                6997 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(809..2089)
                     /label=IL13Ra1
                     /note="IL13Ra1"
     misc_feature    complement(2091..2098)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2103..2129
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2130..2146)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2149..2194)
                     /label=Minimal CMV Promoter
     misc_feature    2172..2194
                     /label=core
                     /note="core"
     promoter        2204..2758
                     /label=CMV Enhancer
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2776..2842
                     /label=EM7 bacterial promoter
     CDS             2856..3515
                     /label=Chloramphenicol resistance
     misc_feature    3632..4554
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    3809
                     /label=C2129
                     /note="C2129"
     polyA_signal    complement(4575..4622)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4646..5440)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5454..5748)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5819..6997)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacct gagaggcttt cttcaggttt tctatcagca
      841 ctacagagtc ggtttcctcc ttggtttgct tctcatagat gtcgtacttc ttccagtgca
      901 gagtatcatc attctggtct ccaaacattt ctttaaaaat cttgccagga tcaggaattg
      961 gagggaatat aataatcttg agccttttta ggtaaagcag gagtactatg attgcacctg
     1021 cgacgatgac tggaacaatg agtaacatgg ttatgtagag tgtggaattg cgcttcttac
     1081 ctatactcat ttcttggctc caattactcc agagtttgtc atcctcatag cataacttat
     1141 ttgttttgac tcttattctg actgtgttca aagtatcagg aagaacacca gggaccatga
     1201 aacaagatgt attctccaca tttctctcaa attctggatt ctcacattta gcctcttgga
     1261 cgtagaaaac attatgtgtc tcagtttggc tgttattgac ttctacttca taaaataggc
     1321 atctgctaat aaaattctgt ggattctccc attgcacata taggtcatca ttgtggaagg
     1381 agaggttttt aatatgtgga ggatcaggtt tcacacggga agttaaaggc actatattga
     1441 aggatggttt aatttttcct gcattatcct tgaccattat ttggacactg tgttgttcaa
     1501 aactggaatc cttcactttg gtcagatcaa aggaacaacc aaagtattgg ccttctctaa
     1561 agatgttttc acattgatga attttttcca ggcttctgtg ccaatagtag agagtatagt
     1621 tagtgtcggg actggtattc cttccaggga gccaagaaca cttcatgtag ctcaggttgt
     1681 gccaaatgca ttgaagctca gtcacagcag actcaggatc accttctggg ggtgagatgc
     1741 atttttcaac caaaatgcta ggcttctcac tctcattggt gctacactgg gaccccactt
     1801 gcagacaaat cctctcattc aggggtactt ctattgaacg acgagtttcc ggagctattt
     1861 tcttatcttg tttgtcgcca aaatgactaa aataccatag actacaattt gagctggctc
     1921 cctcgggtgg attccatgtc catattactg tgcagaggtt ttcaacagag acactcaaat
     1981 ttgtcacagg tggctgagtt tccgtaggcg cggcgccccc gcccccgccc ccgccgccgg
     2041 cgcagagcag cagcgcccac agcccgcaga gccgcgccgg ccactccatg gcgatcgcta
     2101 gctgcaaaaa gaacaagtag cttgtattct atagtgtcac ctaaatgggc gatctgacgg
     2161 ttcactaaac cagctctgct tatataggcc tccctggcgc gccactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcaa
     2761 atcgaattct aatactgttg acaattaatc atcggcatag tatatcggca tagtataata
     2821 cgacaaggtg aggaactaaa cccaggaggc agatcatgga gaaaaaaatc actggatata
     2881 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     2941 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     3001 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     3061 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     3121 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     3181 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     3241 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     3301 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     3361 ccgttttcac tatgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     3421 ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     3481 aacagtactg cgatgagtgg cagggcgggg cgtaagcggg actctggggt tcgaaatgac
     3541 cgaccaagcg acgcccaacc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3601 gaatcagggg ataacgcagg aaagaacatg tcaaacatga gaattacaac ttatatcgta
     3661 tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt
     3721 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
     3781 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
     3841 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
     3901 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
     3961 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
     4021 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     4081 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     4141 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc
     4201 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     4261 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     4321 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     4381 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     4441 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca
     4501 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctattttat
     4561 agtccggaat cgtcacacaa aaaaccaaca cacagatgta atgaaaataa agatatttta
     4621 ttgcggccat cgtgatggct agagttcaga agaactcgtc aagaaggcga tagaaggcga
     4681 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     4741 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     4801 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     4861 gcaagcaggc atcgccgtgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     4921 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     4981 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     5041 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     5101 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     5161 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     5221 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg
     5281 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     5341 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     5401 cggccggaga acctgcgtgc aatccatctt gttcaatcat ggtggcatcg attcccggtc
     5461 aacatccagt acacctccgg caccaccggc ttccccaagg gcgccacgct ctcccaccac
     5521 aacatcctca acaacggcta ctgggtgggc cgcacggtcg gctacacgga gcaggaccgg
     5581 gtgtgtctgc cggtgccctt ctaccactgc ttcggcatgg tcatgggcaa cttgggcgcc
     5641 acctcccacg ccgcctgcat cgtcatcccg gccccgtcct tcgagccggc ggcgacgctg
     5701 gaggcggtgc agcgggagag gtgcacgtcc ctgtacggcg tccccacgaa gccgaattcc
     5761 accacactgg actagatgca tgctcgacag tgtgatggat atctgcagaa ttcggctttc
     5821 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc
     5881 aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc
     5941 taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc
     6001 aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc
     6061 ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg
     6121 tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctgcctgcag
     6181 cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg
     6241 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
     6301 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
     6361 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
     6421 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
     6481 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
     6541 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
     6601 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
     6661 cgggatcaag aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat
     6721 aacccgtaaa gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg
     6781 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     6841 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     6901 accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg
     6961 gcgatgtgcg ctctgcccac tgacgggcac cggagcc
//
