LOCUS       Exported                8228 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(301..2700)
                     /label=IL4Ra
                     /note="IL4Ra"
     misc_feature    complement(2702..2709)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(2710..2721)
                     /label=linker
                     /note="linker"
     misc_feature    complement(2722..3231)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(3232..3318)
                     /label=IL6 sig seq
                     /note="IL6 sig seq"
     misc_feature    3334..3360
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(3361..3377)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(3380..3425)
                     /label=Minimal CMV Promoter
     misc_feature    3403..3425
                     /label=core
                     /note="core"
     promoter        3435..3989
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        4007..4073
                     /label=EM7 bacterial promoter
     CDS             4087..4746
                     /label=Chloramphenicol resistance
     misc_feature    4863..5785
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    5040
                     /label=C2129
                     /note="C2129"
     polyA_signal    complement(5806..5853)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(5877..6671)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(6685..6979)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(7050..8228)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 agagaccctc atgtatgtgg gtcccacgga gacaaagttc acgattttgg gggtctggct
      361 tgagctctga gcattgccag gggcaggatg gaaggatgat gaggatttac tcttctctga
      421 gatgcccgag ggtgccaggg aggccggaca cagactggcc tccagtggaa ccccacctgg
      481 agaggggtct ggggccctca ggggggttgt agggggcgag gacctgtctc cacagcagca
      541 gccacagcaa ggactggcca tgacaggggt ctggccacca tcctcctggc catgacactg
      601 tttcaggtgg ccgcacaggt ggcaggtaag ggctgagtag acaatgccac tgcccaggct
      661 gtccacaagg gggtctgtgg cctgctcctg gggaagtggg ggctttggca tgtcctctac
      721 cttttccccc ggctccagac ccaggtgctc tggggagctg cttgggagat gtgagctctg
      781 cggactgcga ggtggctccc tgtccagtcc aaaggtgaac aaggggacag ggactggggc
      841 agggtcccca gggcagccag gaatgaggtc ttggaaaggc ttatacccct cttccccact
      901 gctagcccca aacccacatt tctctgggga cacagcactg ctggcaagca ggcttgagaa
      961 ggccttgtaa ccagcctctc ctgggggacc caagcccacc accgcactgg cctgggtgcc
     1021 accctgctcc accgcatgta caaactcctg atagccactg gtgggggccg agacgggggc
     1081 tgcagctgcc ccatgctgga ggacatttcg gcggaggatc tgctcccagg tttctggctc
     1141 aggttggggc acagtggttg gctcagagag ctgggggaca cagggcatct cgggttctac
     1201 ttcctccagg tgtctggcca gcagtgggtc tggacccagc tctctgggac acggtgactg
     1261 gctcagggag ttgctgaagc tgcggtaagc agggttgcct gcgatgacga ggggcgtctc
     1321 tgtgcaagtc aggttgtctg gactctgggt cgggctggca ggaggacttg gctccaggtg
     1381 gagaggctgc tccttgcccc agggaggtgc ctccttgggc cctgcacttg ggaactcatc
     1441 ccagggcatg tgagcactcg tacttcccga aggtggaaga aggcatgact cccccatgtc
     1501 ctgctggcaa aagcccccat tctcctctcc gagcaggtcc aggaacaggc tctctgttag
     1561 ccgggccaca atgccctccc ttccctcctg gaagtcatcc ctgctgctct caggcgatgc
     1621 acagaagctc cctttttctt cctctacctc ctcctcctcc tcacactcca ccggggcctc
     1681 aaacaactcc acacatcgca ccacgctgat gctctctggc cagaggactg tcttgctgat
     1741 ctccactggg caccatgctg attttccaga gccctggaaa ggcatctctt tggcagcctt
     1801 gtgaggatct tcatcccttt tcatgttgtg ctccagaaaa cagggcaaga gcttggtaag
     1861 acaattcttc cagtgtgggc acttggctgg ttcctggcct cgggaccgct tctcccactg
     1921 tgacccctga gcatcctgga ttattatagc cacgaggcgg ctgcgggctg ggttgggaat
     1981 ctgatcccac cattctttct taatcttggt gatgctgaca tagcacaaca ggcagacggc
     2041 caggatgaca atgcaggaaa cgctgacgcc cagcaggagg tgctgctcga agggctccct
     2101 gtaggagttg tgccacttgg tgctggggct ccactcactc caggtggtgt tatagcactg
     2161 agcccaggcc ctcacccgtg ccctgtagga aatcccagac ttcagggtgc tggctgcgat
     2221 gcggagggag ggttctaggt aggtcacgtt atagattctg aaatctgccg ggtcgttttc
     2281 actccaaatg ttgactgcat aggtgagatg attatacagg taattgtcag ggggatacgg
     2341 gttgctccag gtcagcagca gagtgtcgga gacattggtg tgaactgtca ggtttcctgg
     2401 ggccctgggt ttcacatgct cgctgggctt gaaggagccc ttccacagca gctgctgccc
     2461 agcccacagg tccagtgtat agttatccgc actgaccacg tcatccatga gcaggtggca
     2521 cacgcacccc gcgcctccgt tgttctcagg gatacacgtg tgggcttcgg agagcagaaa
     2581 aaccagctgg tacaacaggc ggagctcggt gctgcaattg gtgggaccat tcatcttcca
     2641 ctcgcaagta gagatgctca tgtagtcgga gacgcaggtg ggctcctgca agaccttcat
     2701 ggcgatcgcg ccgctcgagc ccgccagaat gcgttcgcac agccgccagc cggtcactcc
     2761 gttgatggtt actcggaaca gcagggagcc gtcggggttg atcaggcgct cgtcgataat
     2821 tttgttgccg ttccacaggg tccctgttac agtgatcttt ttgccgtcga acacggcgat
     2881 gccttcatac ggccgtccga aatagtcgat catgttcggc gtaaccccgt cgattaccag
     2941 tgtgccatag tgcaggatca ccttaaagtg atgatcatcc acagggtaca ccaccttaaa
     3001 aattttttcg atctggccca tttggtcgcc gctcagacct tcatacggga tgatgacatg
     3061 gatgtcgatc ttcagcccat tttcaccgct caggacaatc ctttggatcg gagttacgga
     3121 caccccgaga ttctgaaaca aactggacac acctccctgt tcaaggactt ggtccaggtt
     3181 gtagccggct gtctgtcgcc agtccccaac gaaatcttcg agtgtgaaga ctggggcagg
     3241 gaaggcagca ggcaacacca ggagcaggcc cagggagaag gcaactggac cgaaggcgct
     3301 tgtggagaag gagttcatgg tggctttgct agctgcaaaa agaacaagta gcttgtattc
     3361 tatagtgtca cctaaatggg cgatctgacg gttcactaaa ccagctctgc ttatataggc
     3421 ctccctggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     3481 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3541 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3601 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     3661 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     3721 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     3781 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     3841 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     3901 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     3961 tgggcggtag gcgtgtacgg tgggaggtca aatcgaattc taatactgtt gacaattaat
     4021 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa acccaggagg
     4081 cagatcatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     4141 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     4201 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     4261 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     4321 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     4381 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     4441 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     4501 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     4561 aacgtggcca atatggacaa cttcttcgcc cccgttttca ctatgggcaa atattatacg
     4621 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     4681 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     4741 gcgtaagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc
     4801 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4861 gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa
     4921 gagataaatt gcactgaaat ctagaaatat tttatctgat taataagatg atcttcttga
     4981 gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc
     5041 ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga
     5101 gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact
     5161 aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg
     5221 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     5281 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     5341 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     5401 agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5461 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     5521 aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag
     5581 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
     5641 cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg
     5701 tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca
     5761 tagtaagcca gtatacactc cgctatttta tagtccggaa tcgtcacaca aaaaaccaac
     5821 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagttcag
     5881 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5941 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     6001 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     6061 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccgtg ggtcacgacg
     6121 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     6181 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6241 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6301 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6361 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6421 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6481 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6541 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6601 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6661 tgttcaatca tggtggcatc gattcccggt caacatccag tacacctccg gcaccaccgg
     6721 cttccccaag ggcgccacgc tctcccacca caacatcctc aacaacggct actgggtggg
     6781 ccgcacggtc ggctacacgg agcaggaccg ggtgtgtctg ccggtgccct tctaccactg
     6841 cttcggcatg gtcatgggca acttgggcgc cacctcccac gccgcctgca tcgtcatccc
     6901 ggccccgtcc ttcgagccgg cggcgacgct ggaggcggtg cagcgggaga ggtgcacgtc
     6961 cctgtacggc gtccccacga agccgaattc caccacactg gactagatgc atgctcgaca
     7021 gtgtgatgga tatctgcaga attcggcttt cacgacacct gaaatggaag aaaaaaactt
     7081 tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc
     7141 aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg
     7201 ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc
     7261 gagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg
     7321 gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag
     7381 cgccgcgtcc tccattttga gctgcctgca gcagggccgg gaagcggcca tctttccgct
     7441 cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc
     7501 gcgaggccag gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt
     7561 ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg
     7621 ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc atttacaaga
     7681 ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat ggctagagac
     7741 ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag
     7801 cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc
     7861 gaactctccc acccacttcc aacccgaagc tcgggatcaa gaatcacgta ctgcagccag
     7921 gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa agaggccagg cccgcgggaa
     7981 ccacacacgg cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg
     8041 gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg gagccagtac
     8101 acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt tcaattgccg
     8161 acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca ctgacgggca
     8221 ccggagcc
//
