LOCUS       Exported                5491 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1685..2318
                     /label=TREM2_NM_018965
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1067..1153
                     /label=IL6 sig seq
                     /note="IL6 sig seq"
     misc_feature    1154..1663
                     /label=NanoLuc(Sec)
                     /note="NanoLuc(Sec)"
     misc_feature    1664..1675
                     /label=linker
                     /note="linker"
     misc_feature    1676..1683
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1685..2320
                     /label=TREM2
                     /note="TREM2"
     misc_feature    2321..2328
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2480..2701
                     /label=Sv40 late polyadenylation signal
     promoter        2800..3218
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3226..3292
                     /label=EM7 bacterial promoter
     CDS             3306..4100
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4164..4212
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      4448..4484
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatga actccttctc
     1081 cacaagcgcc ttcggtccag ttgccttctc cctgggcctg ctcctggtgt tgcctgctgc
     1141 cttccctgcc ccagtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
     1201 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
     1261 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
     1321 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
     1381 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
     1441 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
     1501 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
     1561 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
     1621 caacggagtg accggctggc ggctgtgcga acgcattctg gcgggctcga gcggcgcgat
     1681 cgcccacaac accacagtgt tccagggcgt ggcgggccag tccctgcagg tgtcttgccc
     1741 ctatgactcc atgaagcact gggggaggcg caaggcctgg tgccgccagc tgggagagaa
     1801 gggcccatgc cagcgtgtgg tcagcacgca caacttgtgg ctgctgtcct tcctgaggag
     1861 gtggaatggg agcacagcca tcacagacga taccctgggt ggcactctca ccattacgct
     1921 gcggaatcta caaccccatg atgcgggtct ctaccagtgc cagagcctcc atggcagtga
     1981 ggctgacacc ctcaggaagg tcctggtgga ggtgctggca gaccccctgg atcaccggga
     2041 tgctggagat ctctggttcc ccggggagtc tgagagcttc gaggatgccc atgtggagca
     2101 cagcatctcc aggagcctct tggaaggaga aatccccttc ccacccactt ccatccttct
     2161 cctcctggcc tgcatctttc tcatcaagat tctagcagcc agcgccctct gggctgcagc
     2221 ctggcatgga cagaagccag ggacacatcc acccagtgaa ctggactgtg gccatgaccc
     2281 agggtatcag ctccaaactc tgccagggct gagagacacg gtttaaacga attcgggctc
     2341 ggtacccggg gatcctctag agtcgacctg caggcatgca agctgatccg gctgctaaca
     2401 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     2461 ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     2521 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2581 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2641 caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt
     2701 aaaatcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     2761 acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa
     2821 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga
     2881 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2941 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3001 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3061 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3121 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3181 gaggcttttt tggaggccta ggcttttgca aaaagcttaa ttaactgttg acaattaatc
     3241 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa cccaggaggc
     3301 agatcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     3361 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     3421 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     3481 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3541 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     3601 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     3661 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     3721 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     3781 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     3841 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     3901 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     3961 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4021 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4081 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     4141 caacctgcca tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     4201 gttttttgtg tgaatcgata gcgataagga tcctctttgc gcttgcgttt tcccttgtcc
     4261 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     4321 acccggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4861 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     4921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag
     5041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5101 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa
     5161 atacaggaac gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag
     5221 cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata
     5281 cggttaaaat ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta
     5341 cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta
     5401 tggtaaatcc acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc
     5461 acgatgcgtc cggcgtagag gatcgagatc t
//
