LOCUS       Exported                7462 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     CDS             complement(301..2193)
                     /label=RAD21
     misc_feature    complement(2195..2202)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(2203..2214)
                     /label=linker
                     /note="linker"
     misc_feature    complement(2215..2727)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    2741..2767
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2768..2784)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2787..2832)
                     /label=Minimal CMV Promoter
     misc_feature    2810..2832
                     /label=core
                     /note="core"
     promoter        2842..3396
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        3414..3480
                     /label=EM7 bacterial promoter
     CDS             3494..4153
                     /label=Chloramphenicol resistance
     rep_origin      4322..4358
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5040..5087)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(5111..5905)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5919..6213)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(6284..7462)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 tataatatgg aaccttggtc caggtgttgc gatgatgtca ctgtacggtt cttcctgtgt
      361 cagctcaata gcttgctgct ttttaagaac caagaagctg tagaactttg cggcagcttg
      421 ttttctgttc gtatttcgac ataactcaag caaactgata gattcagctc cagttttagc
      481 aagagcacgc tgaagaccat gaagcatctg ctgagtcctt ttgttccatc ttctttcttc
      541 ctgatcttga tcgccccctg atgcatcttc atcctcttcc tcttcatcat cttctttttc
      601 cttctctttc tccttctctt tttctggcag aagttctaac tctggtatta gctgacagat
      661 atttggaggt tcttctgggg gaagctctac aggtggtatt tccatctgct ctacctgctg
      721 aggaggcatc acaggctctg ggtcaatttg tccagctttt cgcttaactc cctgaggtgg
      781 tggtggaggc atagctgact catctatgtt tgttctgctg gcctccatca ctgactcctg
      841 gaggcggctt ggctcttcaa taatgggctc atcgataaca tcacgctgct gatgctgctg
      901 ttgctggtcc tctctaggaa cctctggatt ttcaaattct ttgaggaatt catccaaatt
      961 atctgcctct cctcctttcc tcctttttct aaggtcttct ggtacaagcg gtgtaagaca
     1021 gcgtgtaaag agcttcagta gtctgttatt ccacaaaggc tgagcaggta aagaaaacag
     1081 tttttctact cctcctgtct ctttccacat catcaatttc ttggtgggcg gtgccagatc
     1141 caaagtagta acaatatctg aataatcact aagttgggct ctaattgtct tgctatccaa
     1201 ctctttgaca ctgtcaacaa ttagcttcct cttcctcttg gcttttgttt ctttaacagt
     1261 tatatcaata ggctccaatg caaatgcttc ttcctcattt ggaacaagtg ttgtttgatc
     1321 agtcatggtt ggcattggtt caacgggatc cactgaatca ggactatcag gcccacccat
     1381 tgatacatta tcatcctcat ccatatcgtc atgtgcaggc tgctctggca acatcacccc
     1441 tgcctcagag agggcagggg gatcatcaaa gataccgcca tcattattac taataagttt
     1501 gtcatctaat attccaccat catttccttc tccaaaatta tcatccttat attgatcttc
     1561 atattctaaa tggttaattt tctcattcag attgctggtg ctctgttcag actctaatag
     1621 gaggttagaa gtagtagtgc ttactaacat gtcgtcatcc tcaaaagcac tgccttctct
     1681 cattatctca cgatcatcca ttccaaaatc accaaaatca ttttcttgta aaatactgat
     1741 gttcccaact tcttctctca tggttatctc ttccactcta ctctgattca agctgaactg
     1801 ctgggccaca tcgatgtcat ctaagtcagg cagtggctga tcaaagtcat gaaattcttc
     1861 aggtaaagta atggcattat aagctgcttc ccgattttcc tcaggcaggt caaccacacc
     1921 tggccgaaaa gccatcttta tcttaatgaa tgcttcatta cagtctgcaa gaaggtattt
     1981 ggctttcctg tgatagattc gaactactcc cagtaagaga tgtcctgatg tccgtaatgc
     2041 cattttcacc tttggtgaga tgatactctc cacgctgctc tctaaattac actcgaacac
     2101 atgggctttg gttagcttct tatcccaatg ggccgctagc caaattttgg ccagaggccc
     2161 tcttttactg agaacaaaat gtgcgtagaa catggcgatc gcgccgctcg agcccgccag
     2221 aatgcgttcg cacagccgcc agccggtcac tccgttgatg gttactcgga acagcaggga
     2281 gccgtcgggg ttgatcaggc gctcgtcgat aattttgttg ccgttccaca gggtccctgt
     2341 tacagtgatc tttttgccgt cgaacacggc gatgccttca tacggccgtc cgaaatagtc
     2401 gatcatgttc ggcgtaaccc cgtcgattac cagtgtgcca tagtgcagga tcaccttaaa
     2461 gtgatgatca tccacagggt acaccacctt aaaaattttt tcgatctggc ccatttggtc
     2521 gccgctcaga ccttcatacg ggatgatgac atggatgtcg atcttcagcc cattttcacc
     2581 gctcaggaca atcctttgga tcggagttac ggacaccccg agattctgaa acaaactgga
     2641 cacacctccc tgttcaagga cttggtccag gttgtagccg gctgtctgtc gccagtcccc
     2701 aacgaaatct tcgagtgtga agaccatggt gagcgctagc tgcaaaaaga acaagtagct
     2761 tgtattctat agtgtcacct aaatgggcga tctgacggtt cactaaacca gctctgctta
     2821 tataggcctc cctggcgcgc cactagttat taatagtaat caattacggg gtcattagtt
     2881 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     2941 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     3001 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     3061 gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg
     3121 cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc
     3181 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt
     3241 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3301 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg
     3361 acgcaaatgg gcggtaggcg tgtacggtgg gaggtcaaat cgaattctaa tactgttgac
     3421 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     3481 caggaggcag atcatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     3541 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     3601 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     3661 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     3721 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     3781 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     3841 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     3901 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     3961 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacta tgggcaaata
     4021 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
     4081 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     4141 gggcggggcg taagcgggac tctggggttc gaaatgaccg accaagcgac gcccaaccgg
     4201 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4261 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4321 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4381 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4441 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4501 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4561 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4621 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4681 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4741 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     4801 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4861 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt caagaagatc
     4921 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4981 tggtcatgag attatcaaaa aggatcttca cctagatcct tttatagtcc ggaatcgtca
     5041 cacaaaaaac caacacacag atgtaatgaa aataaagata ttttattgcg gccatcgtga
     5101 tggctagagt tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     5161 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     5221 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     5281 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     5341 cgtgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     5401 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     5461 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     5521 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     5581 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     5641 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     5701 acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
     5761 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     5821 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     5881 cgtgcaatcc atcttgttca atcatggtgg catcgattcc cggtcaacat ccagtacacc
     5941 tccggcacca ccggcttccc caagggcgcc acgctctccc accacaacat cctcaacaac
     6001 ggctactggg tgggccgcac ggtcggctac acggagcagg accgggtgtg tctgccggtg
     6061 cccttctacc actgcttcgg catggtcatg ggcaacttgg gcgccacctc ccacgccgcc
     6121 tgcatcgtca tcccggcccc gtccttcgag ccggcggcga cgctggaggc ggtgcagcgg
     6181 gagaggtgca cgtccctgta cggcgtcccc acgaagccga attccaccac actggactag
     6241 atgcatgctc gacagtgtga tggatatctg cagaattcgg ctttcacgac acctgaaatg
     6301 gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa
     6361 aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca
     6421 cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg
     6481 tactccaaaa gctcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt
     6541 cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg
     6601 cccgctctcc cgagcgccgc gtcctccatt ttgagctgcc tgcagcaggg ccgggaagcg
     6661 gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg
     6721 cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc
     6781 gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg
     6841 cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc
     6901 ccgcatttac aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt
     6961 aaatggctag agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca
     7021 cgcggcggcc ccagcgccca ggccaggcct caactcaagc acgaggcgaa ggggctcctt
     7081 aagcgcaagg cctcgaactc tcccacccac ttccaacccg aagctcggga tcaagaatca
     7141 cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc
     7201 caggcccgcg ggaaccacac acggcactta cctgtgttct ggcggcaaac ccgttgcgaa
     7261 aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa
     7321 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
     7381 cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
     7441 cccactgacg ggcaccggag cc
//
