LOCUS       Exported                7436 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     CDS             complement(809..2701)
                     /label=RAD21
     misc_feature    complement(2703..2710)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2715..2741
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2742..2758)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2761..2806)
                     /label=Minimal CMV Promoter
     misc_feature    2784..2806
                     /label=core
                     /note="core"
     promoter        2816..3370
                     /label=CMV Enhancer
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        3388..3454
                     /label=EM7 bacterial promoter
     CDS             3468..4127
                     /label=Chloramphenicol resistance
     rep_origin      4296..4332
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5014..5061)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(5085..5879)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5893..6187)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(6258..7436)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacta taatatggaa ccttggtcca ggtgttgcga
      841 tgatgtcact gtacggttct tcctgtgtca gctcaatagc ttgctgcttt ttaagaacca
      901 agaagctgta gaactttgcg gcagcttgtt ttctgttcgt atttcgacat aactcaagca
      961 aactgataga ttcagctcca gttttagcaa gagcacgctg aagaccatga agcatctgct
     1021 gagtcctttt gttccatctt ctttcttcct gatcttgatc gccccctgat gcatcttcat
     1081 cctcttcctc ttcatcatct tctttttcct tctctttctc cttctctttt tctggcagaa
     1141 gttctaactc tggtattagc tgacagatat ttggaggttc ttctggggga agctctacag
     1201 gtggtatttc catctgctct acctgctgag gaggcatcac aggctctggg tcaatttgtc
     1261 cagcttttcg cttaactccc tgaggtggtg gtggaggcat agctgactca tctatgtttg
     1321 ttctgctggc ctccatcact gactcctgga ggcggcttgg ctcttcaata atgggctcat
     1381 cgataacatc acgctgctga tgctgctgtt gctggtcctc tctaggaacc tctggatttt
     1441 caaattcttt gaggaattca tccaaattat ctgcctctcc tcctttcctc ctttttctaa
     1501 ggtcttctgg tacaagcggt gtaagacagc gtgtaaagag cttcagtagt ctgttattcc
     1561 acaaaggctg agcaggtaaa gaaaacagtt tttctactcc tcctgtctct ttccacatca
     1621 tcaatttctt ggtgggcggt gccagatcca aagtagtaac aatatctgaa taatcactaa
     1681 gttgggctct aattgtcttg ctatccaact ctttgacact gtcaacaatt agcttcctct
     1741 tcctcttggc ttttgtttct ttaacagtta tatcaatagg ctccaatgca aatgcttctt
     1801 cctcatttgg aacaagtgtt gtttgatcag tcatggttgg cattggttca acgggatcca
     1861 ctgaatcagg actatcaggc ccacccattg atacattatc atcctcatcc atatcgtcat
     1921 gtgcaggctg ctctggcaac atcacccctg cctcagagag ggcaggggga tcatcaaaga
     1981 taccgccatc attattacta ataagtttgt catctaatat tccaccatca tttccttctc
     2041 caaaattatc atccttatat tgatcttcat attctaaatg gttaattttc tcattcagat
     2101 tgctggtgct ctgttcagac tctaatagga ggttagaagt agtagtgctt actaacatgt
     2161 cgtcatcctc aaaagcactg ccttctctca ttatctcacg atcatccatt ccaaaatcac
     2221 caaaatcatt ttcttgtaaa atactgatgt tcccaacttc ttctctcatg gttatctctt
     2281 ccactctact ctgattcaag ctgaactgct gggccacatc gatgtcatct aagtcaggca
     2341 gtggctgatc aaagtcatga aattcttcag gtaaagtaat ggcattataa gctgcttccc
     2401 gattttcctc aggcaggtca accacacctg gccgaaaagc catctttatc ttaatgaatg
     2461 cttcattaca gtctgcaaga aggtatttgg ctttcctgtg atagattcga actactccca
     2521 gtaagagatg tcctgatgtc cgtaatgcca ttttcacctt tggtgagatg atactctcca
     2581 cgctgctctc taaattacac tcgaacacat gggctttggt tagcttctta tcccaatggg
     2641 ccgctagcca aattttggcc agaggccctc ttttactgag aacaaaatgt gcgtagaaca
     2701 tggcgatcgc tagctgcaaa aagaacaagt agcttgtatt ctatagtgtc acctaaatgg
     2761 gcgatctgac ggttcactaa accagctctg cttatatagg cctccctggc gcgccactag
     2821 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     2881 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     2941 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3001 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3061 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3121 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3181 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
     3241 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3301 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
     3361 gtgggaggtc aaatcgaatt ctaatactgt tgacaattaa tcatcggcat agtatatcgg
     3421 catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg gagaaaaaaa
     3481 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     3541 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     3601 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     3661 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     3721 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     3781 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     3841 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     3901 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     3961 acttcttcgc ccccgttttc actatgggca aatattatac gcaaggcgac aaggtgctga
     4021 tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc
     4081 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaagcg ggactctggg
     4141 gttcgaaatg accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata
     4201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4861 attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac
     4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4981 ttcacctaga tccttttata gtccggaatc gtcacacaaa aaaccaacac acagatgtaa
     5041 tgaaaataaa gatattttat tgcggccatc gtgatggcta gagttcagaa gaactcgtca
     5101 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     5161 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     5221 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     5281 ttttccacca tgatattcgg caagcaggca tcgccgtggg tcacgacgag atcctcgccg
     5341 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     5401 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     5461 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     5521 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     5581 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     5641 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     5701 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     5761 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     5821 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     5881 gtggcatcga ttcccggtca acatccagta cacctccggc accaccggct tccccaaggg
     5941 cgccacgctc tcccaccaca acatcctcaa caacggctac tgggtgggcc gcacggtcgg
     6001 ctacacggag caggaccggg tgtgtctgcc ggtgcccttc taccactgct tcggcatggt
     6061 catgggcaac ttgggcgcca cctcccacgc cgcctgcatc gtcatcccgg ccccgtcctt
     6121 cgagccggcg gcgacgctgg aggcggtgca gcgggagagg tgcacgtccc tgtacggcgt
     6181 ccccacgaag ccgaattcca ccacactgga ctagatgcat gctcgacagt gtgatggata
     6241 tctgcagaat tcggctttca cgacacctga aatggaagaa aaaaactttg aaccactgtc
     6301 tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac
     6361 atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc
     6421 gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga gaactaatcg
     6481 aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga
     6541 aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc
     6601 cattttgagc tgcctgcagc agggccggga agcggccatc tttccgctca cgcaactggt
     6661 gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc gaggccaggc
     6721 accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc
     6781 aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc
     6841 ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact atcttgccag
     6901 aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca
     6961 gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg cccaggccag
     7021 gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga actctcccac
     7081 ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt ggaagtaatt
     7141 caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc acacacggca
     7201 cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca
     7261 cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt
     7321 tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc
     7381 aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagcc
//
