LOCUS       Exported                6845 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     misc_feature    809..2110
                     /label=TEAD4
                     /note="TEAD4"
     misc_feature    complement(2112..2119)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2124..2150
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2151..2167)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2170..2215)
                     /label=Minimal CMV Promoter 2
     misc_feature    2193..2215
                     /label=core
                     /note="core"
     promoter        2225..2779
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2797..2863
                     /label=EM7 bacterial promoter
     CDS             2877..3536
                     /label=Chloramphenicol resistance
     rep_origin      3705..3741
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4423..4470)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4494..5288)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5302..5596)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5667..6845)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaactt ctttcaccag cctgtagatg tggtgctgag
      841 ccccgtgctc actggctgac acctcaaaga cataggcaat gcacagcaag gtctcctgtg
      901 tgtctctgtt ggtgaccacc tgcaggatgg tgaagttctc cagcacgctg ttcatcatgt
      961 acttctcagg gaggtgcttg agcttgtgga tgaagttgat catgtactca cagagcgggg
     1021 accggtggat gcggtaagag tagtgtccat tctcatagcg agcatactct gtctcaactt
     1081 tctccaccac ctgcttgccg aaagagcaga ccttcgtgga gcaggtgatg atcatgttct
     1141 cggggctctc atactggctg gagaccccat agaaggagct gccttcatcc tcgatgttgg
     1201 tgttgaggtc tgcccagaac ttcacaagaa aaaaggcatt ggagggtccc cgttcgaaga
     1261 gatccttgag tccacccttt ttctccggga atttgtcata gatttggcgg atgtccacgg
     1321 cttcgaggta ggggtcgctg tagcttgggc tggactggcc aatgtgcacg aacaggtgct
     1381 tgttgtacgt gtccgggtcc tgctgctgct ccaggaaggc agagaactcc aacatccaga
     1441 gcttggagct ggccacgctg cggccctgcc atgggggtgc cgggggcgca gagggcgatg
     1501 gggcgggccc tgcaggagac tcaaaccctg gcagaggcag cggaggctgg acagcatagg
     1561 tttgctgaga gaaaggcttc acatcatggg acgttccggc ttggcctggc aaagctcctt
     1621 gccaaaaccc tgagactgct gggcggccgg ggccccgggc gagggccatg ctactgtgga
     1681 aggccgtggc ggagatgatc tgtgcagacg acatggcagc catgctctgc agggccttgt
     1741 ccttagctgc ctggtccttt agcttggcct ggatctcgcg agctttgcga cgagccagca
     1801 cctggatgtg gctggagacc tgcttcctgg tgcgggtctt ccctgtccgg agcttgatgt
     1861 agcgggcaat cagctcgttc cgaccataca tcttgccctc gtccgacagg atgattttgc
     1921 gcctgccaca gggcgggtag atggcgaggg cctcctggaa actctgctca atatccgggc
     1981 tccacacgcc ctctgcgtca ttgtcgatgg gcttgtccag tgcctgactg cccccagagg
     2041 cggtgctccc ctcaggggag gtgggagagc tccactcgtt ggaggtaatg gtgccggccg
     2101 tgccctccat ggcgatcgct agctgcaaaa agaacaagta gcttgtattc tatagtgtca
     2161 cctaaatggg cgatctgacg gttcactaaa ccagctctgc ttatataggc ctccctggcg
     2221 cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2281 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2341 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2401 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2461 tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2521 ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2581 tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc
     2641 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2701 tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag
     2761 gcgtgtacgg tgggaggtca aatcgaattc taatactgtt gacaattaat catcggcata
     2821 gtatatcggc atagtataat acgacaaggt gaggaactaa acccaggagg cagatcatgg
     2881 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     2941 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     3001 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     3061 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     3121 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     3181 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     3241 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     3301 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     3361 atatggacaa cttcttcgcc cccgttttca ctatgggcaa atattatacg caaggcgaca
     3421 aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg
     3481 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaagcgg
     3541 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag
     3601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4081 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4141 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4201 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4261 aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg
     4321 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4381 aaaaggatct tcacctagat ccttttatag tccggaatcg tcacacaaaa aaccaacaca
     4441 cagatgtaat gaaaataaag atattttatt gcggccatcg tgatggctag agttcagaag
     4501 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     4561 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     4621 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     4681 aagcggccat tttccaccat gatattcggc aagcaggcat cgccgtgggt cacgacgaga
     4741 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     4801 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     4861 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     4921 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     4981 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     5041 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     5101 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     5161 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     5221 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     5281 tcaatcatgg tggcatcgat tcccggtcaa catccagtac acctccggca ccaccggctt
     5341 ccccaagggc gccacgctct cccaccacaa catcctcaac aacggctact gggtgggccg
     5401 cacggtcggc tacacggagc aggaccgggt gtgtctgccg gtgcccttct accactgctt
     5461 cggcatggtc atgggcaact tgggcgccac ctcccacgcc gcctgcatcg tcatcccggc
     5521 cccgtccttc gagccggcgg cgacgctgga ggcggtgcag cgggagaggt gcacgtccct
     5581 gtacggcgtc cccacgaagc cgaattccac cacactggac tagatgcatg ctcgacagtg
     5641 tgatggatat ctgcagaatt cggctttcac gacacctgaa atggaagaaa aaaactttga
     5701 accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc aaattccaag
     5761 gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc agtgtgggga
     5821 aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca aaagctcgag
     5881 aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga agcgacggct
     5941 gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc
     6001 cgcgtcctcc attttgagct gcctgcagca gggccgggaa gcggccatct ttccgctcac
     6061 gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca cggcggcgcg
     6121 aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat tctcggtggc
     6181 cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg
     6241 cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt tacaagacta
     6301 tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc tagagactta
     6361 tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg gccccagcgc
     6421 ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca aggcctcgaa
     6481 ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg cagccaggtg
     6541 gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc gcgggaacca
     6601 cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg
     6661 actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg
     6721 acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca attgccgacc
     6781 cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg acgggcaccg
     6841 gagcc
//
