LOCUS       Exported                6871 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    301..1602
                     /label=TEAD4
                     /note="TEAD4"
     misc_feature    complement(1604..1611)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(1612..1623)
                     /label=linker
                     /note="linker"
     misc_feature    complement(1624..2136)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    2150..2176
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2177..2193)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2196..2241)
                     /label=Minimal CMV Promoter 2
     misc_feature    2219..2241
                     /label=core
                     /note="core"
     promoter        2251..2805
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2823..2889
                     /label=EM7 bacterial promoter
     CDS             2903..3562
                     /label=Chloramphenicol resistance
     rep_origin      3731..3767
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4449..4496)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4520..5314)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5328..5622)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5693..6871)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 ttctttcacc agcctgtaga tgtggtgctg agccccgtgc tcactggctg acacctcaaa
      361 gacataggca atgcacagca aggtctcctg tgtgtctctg ttggtgacca cctgcaggat
      421 ggtgaagttc tccagcacgc tgttcatcat gtacttctca gggaggtgct tgagcttgtg
      481 gatgaagttg atcatgtact cacagagcgg ggaccggtgg atgcggtaag agtagtgtcc
      541 attctcatag cgagcatact ctgtctcaac tttctccacc acctgcttgc cgaaagagca
      601 gaccttcgtg gagcaggtga tgatcatgtt ctcggggctc tcatactggc tggagacccc
      661 atagaaggag ctgccttcat cctcgatgtt ggtgttgagg tctgcccaga acttcacaag
      721 aaaaaaggca ttggagggtc cccgttcgaa gagatccttg agtccaccct ttttctccgg
      781 gaatttgtca tagatttggc ggatgtccac ggcttcgagg taggggtcgc tgtagcttgg
      841 gctggactgg ccaatgtgca cgaacaggtg cttgttgtac gtgtccgggt cctgctgctg
      901 ctccaggaag gcagagaact ccaacatcca gagcttggag ctggccacgc tgcggccctg
      961 ccatgggggt gccgggggcg cagagggcga tggggcgggc cctgcaggag actcaaaccc
     1021 tggcagaggc agcggaggct ggacagcata ggtttgctga gagaaaggct tcacatcatg
     1081 ggacgttccg gcttggcctg gcaaagctcc ttgccaaaac cctgagactg ctgggcggcc
     1141 ggggccccgg gcgagggcca tgctactgtg gaaggccgtg gcggagatga tctgtgcaga
     1201 cgacatggca gccatgctct gcagggcctt gtccttagct gcctggtcct ttagcttggc
     1261 ctggatctcg cgagctttgc gacgagccag cacctggatg tggctggaga cctgcttcct
     1321 ggtgcgggtc ttccctgtcc ggagcttgat gtagcgggca atcagctcgt tccgaccata
     1381 catcttgccc tcgtccgaca ggatgatttt gcgcctgcca cagggcgggt agatggcgag
     1441 ggcctcctgg aaactctgct caatatccgg gctccacacg ccctctgcgt cattgtcgat
     1501 gggcttgtcc agtgcctgac tgcccccaga ggcggtgctc ccctcagggg aggtgggaga
     1561 gctccactcg ttggaggtaa tggtgccggc cgtgccctcc atggcgatcg cgccgctcga
     1621 gcccgccaga atgcgttcgc acagccgcca gccggtcact ccgttgatgg ttactcggaa
     1681 cagcagggag ccgtcggggt tgatcaggcg ctcgtcgata attttgttgc cgttccacag
     1741 ggtccctgtt acagtgatct ttttgccgtc gaacacggcg atgccttcat acggccgtcc
     1801 gaaatagtcg atcatgttcg gcgtaacccc gtcgattacc agtgtgccat agtgcaggat
     1861 caccttaaag tgatgatcat ccacagggta caccacctta aaaatttttt cgatctggcc
     1921 catttggtcg ccgctcagac cttcatacgg gatgatgaca tggatgtcga tcttcagccc
     1981 attttcaccg ctcaggacaa tcctttggat cggagttacg gacaccccga gattctgaaa
     2041 caaactggac acacctccct gttcaaggac ttggtccagg ttgtagccgg ctgtctgtcg
     2101 ccagtcccca acgaaatctt cgagtgtgaa gaccatggtg agcgctagct gcaaaaagaa
     2161 caagtagctt gtattctata gtgtcaccta aatgggcgat ctgacggttc actaaaccag
     2221 ctctgcttat ataggcctcc ctggcgcgcc actagttatt aatagtaatc aattacgggg
     2281 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     2341 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2401 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2461 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     2521 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     2581 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
     2641 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     2761 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtcaaatc gaattctaat
     2821 actgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     2881 aactaaaccc aggaggcaga tcatggagaa aaaaatcact ggatatacca ccgttgatat
     2941 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     3001 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     3061 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     3121 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     3181 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     3241 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     3301 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     3361 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcactat
     3421 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     3481 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     3541 tgagtggcag ggcggggcgt aagcgggact ctggggttcg aaatgaccga ccaagcgacg
     3601 cccaaccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc
     4321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4381 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg
     4441 gaatcgtcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     4501 ccatcgtgat ggctagagtt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     4561 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     4621 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     4681 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     4741 aggcatcgcc gtgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     4801 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     4861 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     4921 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     4981 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     5041 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     5101 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     5161 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     5221 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     5281 gagaacctgc gtgcaatcca tcttgttcaa tcatggtggc atcgattccc ggtcaacatc
     5341 cagtacacct ccggcaccac cggcttcccc aagggcgcca cgctctccca ccacaacatc
     5401 ctcaacaacg gctactgggt gggccgcacg gtcggctaca cggagcagga ccgggtgtgt
     5461 ctgccggtgc ccttctacca ctgcttcggc atggtcatgg gcaacttggg cgccacctcc
     5521 cacgccgcct gcatcgtcat cccggccccg tccttcgagc cggcggcgac gctggaggcg
     5581 gtgcagcggg agaggtgcac gtccctgtac ggcgtcccca cgaagccgaa ttccaccaca
     5641 ctggactaga tgcatgctcg acagtgtgat ggatatctgc agaattcggc tttcacgaca
     5701 cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc
     5761 caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt
     5821 cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta
     5881 aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact
     5941 ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg
     6001 actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctgcct gcagcagggc
     6061 cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc
     6121 agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag
     6181 actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc
     6241 gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca
     6301 gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc
     6361 aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc
     6421 agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag
     6481 gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat
     6541 caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg
     6601 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc
     6661 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
     6721 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc
     6781 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
     6841 tgcgctctgc ccactgacgg gcaccggagc c
//
