LOCUS       Exported                5458 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2109..2384
                     /label=SUMO3_GG_Cterm
     source          2385..2386
                     /label=SUMO1_GG_Cterm
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    complement(192..254)
                     /label=MCS 2
     misc_feature    192..199
                     /label=NotI
                     /note="NotI"
     misc_feature    197..218
                     /label=HnHc_dR4_1
                     /note="HnHc_dR4_1"
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     misc_feature    620..638
                     /label=HnHc_dR4_2
                     /note="HnHc_dR4_2"
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1170..2060
                     /label=HaloTag
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    complement(1210..1230)
                     /label=HTnR
                     /note="HTnR"
     misc_feature    1607..1625
                     /label=HnHc_dR4_3
                     /note="HnHc_dR4_3"
     misc_feature    2061..2099
                     /label=HT7 Linker N terminal
     misc_feature    2073..2093
                     /label=TEV protease recognition sequence
     misc_feature    2100..2107
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2109..2386
                     /label=SUMO3 - truncation after GG motif
                     /note="SUMO3 - truncation after GG motif, no C-terminal 
                     Valine"
     misc_feature    2388..2395
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2491..2712
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(2736..2769)
                     /label=Cre site
                     /note="Cre site"
     promoter        2778..2844
                     /label=EM7 bacterial promoter
     CDS             2858..3652
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    3232..3249
                     /label=HnHc_dR4_5
                     /note="HnHc_dR4_5"
     rep_origin      3807..3843
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    3829..3851
                     /label=HnHc_dR4_6
                     /note="HnHc_dR4_6"
     misc_feature    4425..4443
                     /label=HnHc_dR4_7
                     /note="HnHc_dR4_7"
     polyA_signal    complement(4525..4572)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(4596..4994)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(5019..5437)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    5028..5045
                     /label=HnHc_dR4_8
                     /note="HnHc_dR4_8"
     misc_feature    join(5429..5458,1..3)
                     /label=RSGBFB15 (primer)
                     /note="RSGBFB15 (primer)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc aaagccacca tggcagaaat cggtactggc tttccattcg
     1201 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     1261 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     1321 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     1381 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     1441 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     1501 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     1561 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     1621 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     1681 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     1741 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     1801 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     1861 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     1921 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     1981 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     2041 cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag agcgataacg
     2101 cgatcgccat gtccgaggag aagcccaagg agggtgtgaa gacagagaat gaccacatca
     2161 acctgaaggt ggccgggcag gacggctccg tggtgcagtt caagatcaag aggcacacgc
     2221 cgctgagcaa gctgatgaag gcctactgcg agaggcaggg cttgtcaatg aggcagatca
     2281 gattcaggtt cgacgggcag ccaatcaatg aaactgacac tccagcacag ctggagatgg
     2341 aggacgagga caccatcgac gtgttccagc agcagacggg aggttaagtt taaacgaatt
     2401 cgggctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc tgatttggct
     2461 gctgccaccg ctgagcaagg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     2521 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2581 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     2641 attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag taaaacctct
     2701 acaaatgtgg taaaatctta attaagaatt ctaatataac ttcgtatagc atacattata
     2761 cgaagttatg gatccactgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     2821 tacgacaagg tgaggaacta aacccaggag gcagatcatg attgaacaag atggattgca
     2881 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     2941 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     3001 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     3061 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3121 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3181 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     3241 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     3301 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3361 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     3421 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3481 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3541 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3601 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gattcgaaat
     3661 gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat acggttatcc
     3721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     4261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4381 agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4501 atccttttat agtccggaat cgtcacacaa aaaaccaaca cacagatgta atgaaaataa
     4561 agatatttta ttgcggccat cgtgatggct agagtttagc cctcccacac ataaccagag
     4621 ggcagcaatt cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatggct
     4681 ttgatcccag gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg
     4741 cccctgttct catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca
     4801 gcagtgccca gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca
     4861 ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag
     4921 atggggatgc tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac
     4981 aaaggcttgg ccatggtggc gctagtgtca gaagaatcga gctttttgca aaagcctagg
     5041 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     5101 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     5161 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     5221 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     5281 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     5341 cctaactgac acacattcca cagctggttc tttccgcctc agaaggtacc taaccaagtt
     5401 cctctttcag aggttatttc aggccatggt gctgcgcaag acgcgttaat taagacgt
//
