LOCUS       Exported                5461 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2109..2387
                     /label=SUMO2_GG_Cterm
     source          2388..2389
                     /label=SUMO1_GG_Cterm
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    complement(192..254)
                     /label=MCS 2
     misc_feature    192..199
                     /label=NotI
                     /note="NotI"
     misc_feature    197..218
                     /label=HnHc_dR4_1
                     /note="HnHc_dR4_1"
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     misc_feature    620..638
                     /label=HnHc_dR4_2
                     /note="HnHc_dR4_2"
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1170..2060
                     /label=HaloTag
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    complement(1210..1230)
                     /label=HTnR
                     /note="HTnR"
     misc_feature    1607..1625
                     /label=HnHc_dR4_3
                     /note="HnHc_dR4_3"
     misc_feature    2061..2099
                     /label=HT7 Linker N terminal
     misc_feature    2073..2093
                     /label=TEV protease recognition sequence
     misc_feature    2100..2107
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2109..2389
                     /label=SUMO2 - truncation after GG motif
                     /note="SUMO2 - truncation after GG motif, no C-terminal 
                     Valine"
     misc_feature    2391..2398
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2494..2715
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(2739..2772)
                     /label=Cre site
                     /note="Cre site"
     promoter        2781..2847
                     /label=EM7 bacterial promoter
     CDS             2861..3655
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    3235..3252
                     /label=HnHc_dR4_5
                     /note="HnHc_dR4_5"
     rep_origin      3810..3846
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    3832..3854
                     /label=HnHc_dR4_6
                     /note="HnHc_dR4_6"
     misc_feature    4428..4446
                     /label=HnHc_dR4_7
                     /note="HnHc_dR4_7"
     polyA_signal    complement(4528..4575)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(4599..4997)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(5022..5440)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    5031..5048
                     /label=HnHc_dR4_8
                     /note="HnHc_dR4_8"
     misc_feature    join(5432..5461,1..3)
                     /label=RSGBFB15 (primer)
                     /note="RSGBFB15 (primer)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc aaagccacca tggcagaaat cggtactggc tttccattcg
     1201 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     1261 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     1321 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     1381 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     1441 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     1501 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     1561 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     1621 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     1681 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     1741 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     1801 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     1861 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     1921 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     1981 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     2041 cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag agcgataacg
     2101 cgatcgccat ggccgacgaa aagcccaagg aaggagtcaa gactgagaac aacgatcata
     2161 ttaatttgaa ggtggcgggg caggatggtt ctgtggtgca gtttaagatt aagaggcata
     2221 caccacttag taaactaatg aaagcctatt gtgaacgaca gggattgtca atgaggcaga
     2281 tcagattccg atttgacggg caaccaatca atgaaacaga cacacctgca cagttggaaa
     2341 tggaggatga agatacaatt gatgtgttcc aacagcagac gggaggttaa gtttaaacga
     2401 attcgggctc ggtacccggg gatcctctag agtcgacctg caggcatgca agctgatttg
     2461 gctgctgcca ccgctgagca aggccgcttc gagcagacat gataagatac attgatgagt
     2521 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     2581 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     2641 ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc
     2701 tctacaaatg tggtaaaatc ttaattaaga attctaatat aacttcgtat agcatacatt
     2761 atacgaagtt atggatccac tgttgacaat taatcatcgg catagtatat cggcatagta
     2821 taatacgaca aggtgaggaa ctaaacccag gaggcagatc atgattgaac aagatggatt
     2881 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     2941 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3001 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     3061 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3121 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3181 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3241 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3301 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3361 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     3421 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3481 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3541 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     3601 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgattcga
     3661 aatgaccgac caagcgacgc ccaaccggta tcagctcact caaaggcggt aatacggtta
     3721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3961 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4021 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4081 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4141 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4201 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     4261 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4321 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4381 cgcagaaaaa aaggatttca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4441 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4501 tagatccttt tatagtccgg aatcgtcaca caaaaaacca acacacagat gtaatgaaaa
     4561 taaagatatt ttattgcggc catcgtgatg gctagagttt agccctccca cacataacca
     4621 gagggcagca attcacgaat cccaactgcc gtcggctgtc catcactgtc cttcactatg
     4681 gctttgatcc caggatgcag atcgagaagc acctgtcggc accgtccgca ggggctcaag
     4741 atgcccctgt tctcatttcc gatcgcgacg atacaagtca ggttgccagc tgccgcagca
     4801 gcagcagtgc ccagcaccac gagttctgca caaggtcccc cagtaaaatg atatacattg
     4861 acaccagtga agatgcggcc gtcgctagag agagctgcgc tggcgacgct gtagtcttca
     4921 gagatgggga tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga
     4981 gacaaaggct tggccatggt ggcgctagtg tcagaagaat cgagcttttt gcaaaagcct
     5041 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
     5101 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     5161 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
     5221 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
     5281 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     5341 caccctaact gacacacatt ccacagctgg ttctttccgc ctcagaaggt acctaaccaa
     5401 gttcctcttt cagaggttat ttcaggccat ggtgctgcgc aagacgcgtt aattaagacg
     5461 t
//
