LOCUS       Exported                7523 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     CDS             1065..3980
                     /label=CSF1R
                     /note="Homo sapiens colony stimulating factor 1 receptor, 
                     formerly McDonough feline sarcoma viral (v-fms) oncogene 
                     homolog (CSF1R), mRNA
                     This insert was automatically updated on 2/14/2007. Any 
                     NCBI Synonyms, Description and the comparison to the 
                     accession number were all automatically updated from NCBI."
     misc_feature    3981..3984
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    3987..4001
                     /label=linker
                     /note="linker"
     misc_feature    4002..4511
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    4648..4869
                     /label=Sv40 late polyadenylation signal
     promoter        4968..5386
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        5394..5460
                     /label=EM7 bacterial promoter
     CDS             5474..6268
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    6332..6380
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    6423..7345
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    6600
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgggc ccaggagttc
     1081 tgctgctcct gctggtggcc acagcttggc atggtcaggg aatcccagtg atagagccca
     1141 gtgtccctga gctggtcgtg aagccaggag caacggtgac cttgcgatgt gtgggcaatg
     1201 gcagcgtgga atgggatggc cccccatcac ctcactggac cctgtactct gatggctcca
     1261 gcagcatcct cagcaccaac aacgctacct tccaaaacac ggggacctat cgctgcactg
     1321 agcctggaga ccccctggga ggcagcgccg ccatccacct ctatgtcaaa gaccctgccc
     1381 ggccctggaa cgtgctagca caggaggtgg tcgtgttcga ggaccaggac gcactactgc
     1441 cctgtctgct cacagacccg gtgctggaag caggcgtctc gctggtgcgt gtgcgtggcc
     1501 ggcccctcat gcgccacacc aactactcct tctcgccctg gcatggcttc accatccaca
     1561 gggccaagtt cattcagagc caggactatc aatgcagtgc cctgatgggt ggcaggaagg
     1621 tgatgtccat cagcatccgg ctgaaagtgc agaaagtcat cccagggccc ccagccttga
     1681 cactggtgcc tgcagagctg gtgcggattc gaggggaggc tgcccagatc gtgtgctcag
     1741 ccagcagcgt tgatgttaac tttgatgtct tcctccaaca caacaacacc aagctcgcaa
     1801 tccctcaaca atctgacttt cataataacc gttaccaaaa agtcctgacc ctcaacctcg
     1861 atcaagtaga tttccaacat gccggcaact actcctgcgt ggccagcaac gtgcagggca
     1921 agcactccac ctccatgttc ttccgggtgg tagagagtgc ctacttgaac ttgagctctg
     1981 agcagaacct catccaggag gtgaccgtgg gggaggggct caacctcaaa gtcatggtgg
     2041 aggcctaccc aggcctgcaa ggttttaact ggacctacct gggacccttt tctgaccacc
     2101 agcctgagcc caagcttgct aatgctacca ccaaggacac atacaggcac accttcaccc
     2161 tctctctgcc ccgcctgaag ccctctgagg ctggccgcta ctccttcctg gccagaaacc
     2221 caggaggctg gagagctctg acgtttgagc tcacccttcg atacccccca gaggtaagcg
     2281 tcatatggac attcatcaac ggctctggca cccttttgtg tgctgcctct gggtaccccc
     2341 agcccaacgt gacatggctg cagtgcagtg gccacactga taggtgtgat gaggcccaag
     2401 tgctgcaggt ctgggatgac ccataccctg aggtcctgag ccaggagccc ttccacaagg
     2461 tgacggtgca gagcctgctg actgttgaga ccttagagca caaccaaacc tacgagtgca
     2521 gggcccacaa cagcgtgggg agtggctcct gggccttcat acccatctct gcaggagccc
     2581 acacgcatcc cccggatgag ttcctcttca caccagtggt ggtcgcctgc atgtccatca
     2641 tggccttgct gctgctgctg ctcctgctgc tattgtacaa gtataagcag aagcccaagt
     2701 accaggtccg ctggaagatc atcgagagct atgagggcaa cagttatact ttcatcgacc
     2761 ccacgcagct gccttacaac gagaagtggg agttcccccg gaacaacctg cagtttggta
     2821 agaccctcgg agctggagcc tttgggaagg tggtggaggc cacggccttt ggtctgggca
     2881 aggaggatgc tgtcctgaag gtggctgtga agatgctgaa gtccacggcc catgctgatg
     2941 agaaggaggc cctcatgtcc gagctgaaga tcatgagcca cctgggccag cacgagaaca
     3001 tcgtcaacct tctgggagcc tgtacccatg gaggccctgt actggtcatc acggagtact
     3061 gttgctatgg cgacctgctc aactttctgc gaaggaaggc tgaggccatg ctgggaccca
     3121 gcctgagccc cggccaggac cccgagggag gcgtcgacta taagaacatc cacctcgaga
     3181 agaaatatgt ccgcagggac agtggcttct ccagccaggg tgtggacacc tatgtggaga
     3241 tgaggcctgt ctccacttct tcaaatgact ccttctctga gcaagacctg gacaaggagg
     3301 atggacggcc cctggagctc cgggacctgc ttcacttctc cagccaagta gcccagggca
     3361 tggccttcct cgcttccaag aattgcatcc accgggacgt ggcagcgcgt aacgtgctgt
     3421 tgaccaatgg tcatgtggcc aagattgggg acttcgggct ggctagggac atcatgaatg
     3481 actccaacta cattgtcaag ggcaatgccc gcctgcctgt gaagtggatg gccccagaga
     3541 gcatctttga ctgtgtctac acggttcaga gcgacgtctg gtcctatggc atcctcctct
     3601 gggagatctt ctcacttggg ctgaatccct accctggcat cctggtgaac agcaagttct
     3661 ataaactggt gaaggatgga taccaaatgg cccagcctgc atttgcccca aagaatatat
     3721 acagcatcat gcaggcctgc tgggccttgg agcccaccca cagacccacc ttccagcaga
     3781 tctgctcctt ccttcaggag caggcccaag aggacaggag agagcgggac tataccaatc
     3841 tgccgagcag cagcagaagc ggtggcagcg gcagcagcag cagtgagctg gaggaggaga
     3901 gctctagtga gcacctgacc tgctgcgagc aaggggatat cgcccagccc ttgctgcagc
     3961 ccaacaacta tcagttctgc gtttctctcg gctcgagcgg cgtcttcaca ctcgaagatt
     4021 tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag
     4081 gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc
     4141 tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
     4201 gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc
     4261 atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca
     4321 tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
     4381 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg
     4441 acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac
     4501 gcattctggc gtaaggccgc gactctagag tcgacctgca ggcatgcaag ctgatccggc
     4561 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     4621 ataacccctt ggggcggccg cttcgagcag acatgataag atacattgat gagtttggac
     4681 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4741 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     4801 ttatgtttca ggttcagggg gagatgtggg aggttttttt aagcaagtaa aacctctaca
     4861 aatgtggtaa aatcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     4921 ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg cagcaccatg
     4981 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
     5041 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5101 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5161 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5221 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5281 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5341 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttaatt aactgttgac
     5401 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     5461 caggaggcag atcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5521 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5581 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5641 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5701 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5761 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5821 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5881 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5941 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6001 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6061 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6121 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6181 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6241 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6301 gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt cattacatct
     6361 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc cgctcatgcg atgataagct
     6421 gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa
     6481 gagataaatt gcactgaaat ctagaaatat tttatctgat taataagatg atcttcttga
     6541 gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc
     6601 ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga
     6661 gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact
     6721 aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg
     6781 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     6841 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     6901 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     6961 agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7021 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     7081 aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag
     7141 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
     7201 cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg
     7261 tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca
     7321 tagtaagcca gtatacactc cgctagcgct gatgtccggc ggtgcttttg ccgttacgca
     7381 ccaccccgtc atgagattac gcgttttagc ggtgcgtaca attaagggat tatggtaaat
     7441 ccacttactg tctgccctcg tagccatcga gataaaccgc agtactccgg ccacgatgcg
     7501 tccggcgtag aggatcgaga tct
//
