LOCUS       Exported                7995 bp ds-DNA     linear   SYN 30-MAY-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural protein sequence
  ORGANISM  unspecified
REFERENCE   1  (residues 1 to 7995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC32K(pF-Nc5K3)      5119 bp    DNA   circular         
              12/7/2015

            DEFINITION  Current Name: pFC32K (pF-Nc5K3) Old/Alternate 
            Name(s):pF-Nc5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="unspecified"
     region          1..742
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
                     /label=CMV immediate-early enhancer/promoter
     region          857..989
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
                     /label=chimeric intron
     region          1033..1052
                     /label=T7 RNA Polymerase promoter
     region          1056..1063
                     /note="Used in Flexi Vector Cloning"
                     /label=SgfI Site
     region          1065..4316
                     /label=HDAC4_NM_006037
     region          1065..4316
                     /note="HDAC4"
                     /label=HDAC4
     region          4323..4337
                     /label=C Terminal NanoLuc linker
     region          4326..4337
                     /label=N Terminal NanoLuc linker
     region          4338..4847
                     /label=C terminal NanoLuc
     region          4984..5205
                     /label=Sv40 late polyadenylation signal
     region          5304..5722
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
                     /label=SV40 enhancer and early promoter
     region          5377..5613
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
                     /label=SV40 Enhancer
     region          5620..5685
                     /label=Sv40 Min Ori
     region          5730..5796
                     /label=EM7 bacterial promoter
     region          5810..6604
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
                     /label=Neo-Kan Resistance
     region          6668..6716
                     /label=Synthetic PolyAdenylation Signal
     region          6952..6988
                     /note="The actual origin is the first base."
                     /label=ColE1-derived plasmid replication origin
     region          7659..7944
                     /note="Prevents multimerization"
                     /label=Cer Region
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgagc tcccaaagcc
     1081 atccagatgg actttctggc cgagaccagc cagtggagct gctgaatcct gcccgcgtga
     1141 accacatgcc cagcacggtg gatgtggcca cggcgctgcc tctgcaagtg gccccctcgg
     1201 cagtgcccat ggacctgcgc ctggaccacc agttctcact gcctgtggca gagccggccc
     1261 tgcgggagca gcagctgcag caggagctcc tggcgctcaa gcagaagcag cagatccaga
     1321 ggcagatcct catcgctgag ttccagaggc agcacgagca gctctcccgg cagcacgagg
     1381 cgcagctcca cgagcacatc aagcaacaac aggagatgct ggccatgaag caccagcagg
     1441 agctgctgga acaccagcgg aagctggaga ggcaccgcca ggagcaggag ctggagaagc
     1501 agcaccggga gcagaagctg cagcagctca agaacaagga gaagggcaaa gagagtgccg
     1561 tggccagcac agaagtgaag atgaagttac aagaatttgt cctcaataaa aagaaggcgc
     1621 tggcccaccg gaatctgaac cactgcattt ccagcgaccc tcgctactgg tacgggaaaa
     1681 cgcagcacag ttcccttgac cagagttctc caccccagag cggagtgtcg acctcctata
     1741 accacccggt cctgggaatg tacgacgcca aagatgactt ccctcttagg aaaacagctt
     1801 ctgaaccgaa tctgaaatta cggtccaggc taaagcagaa agtggccgaa agacggagca
     1861 gccccctgtt acgcaggaaa gacgggccag tggtcactgc tctaaaaaag cgtccgttgg
     1921 atgtcacaga ctccgcgtgc agcagcgccc caggctccgg acccagctca cccaacaaca
     1981 gctccgggag cgtcagcgcg gagaacggta tcgcgcccgc cgtccccagc atcccggcgg
     2041 agacgagttt ggcgcacaga cttgtggcac gagaaggctc ggccgctcca cttcccctct
     2101 acacatcgcc atccttgccc aacatcacgc tgggcctgcc tgccaccggc ccctctgcgg
     2161 gcacggcggg ccagcaggac gccgagagac tcacccttcc cgccctccag cagaggctct
     2221 cccttttccc cggcacccac ctcactccct acctgagcac ctcgcccttg gagcgggacg
     2281 gaggggcagc gcacagccct cttctgcagc acatggtctt actggagcag ccgccggcac
     2341 aagcacccct cgtcacaggc ctgggagcac tgcccctcca cgcacagtcc ttggttggtg
     2401 cagaccgggt gtccccctcc atccacaagc tgcggcagca ccgcccactg gggcggaccc
     2461 agtcggcccc gctgccccag aacgcccagg ctctgcagca cctggtcatc cagcagcagc
     2521 atcagcagtt tctggagaaa cacaagcagc agttccagca gcagcaactg cagatgaaca
     2581 agatcatccc caagccaagc gagccagccc ggcagccgga gagccacccg gaggagacgg
     2641 aggaggagct ccgtgagcac caggctctgc tggacgagcc ctacctggac cggctgccgg
     2701 ggcagaagga ggcgcacgca caggccggcg tgcaggtgaa gcaggagccc attgagagcg
     2761 atgaggaaga ggcagagccc ccacgggagg tggagccggg ccagcgccag cccagtgagc
     2821 aggagctgct cttcagacag caagccctcc tgctggagca gcagcggatc caccagctga
     2881 ggaactacca ggcgtccatg gaggccgccg gcatccccgt gtccttcggc ggccacaggc
     2941 ctctgtcccg ggcgcagtcc tcacccgcgt ctgccacctt ccccgtgtct gtgcaggagc
     3001 cccccaccaa gccgaggttc acgacaggcc tcgtgtatga cacgctgatg ctgaagcacc
     3061 agtgcacctg cgggagtagc agcagccacc ccgagcacgc cgggaggatc cagagcatct
     3121 ggtcccgcct gcaggagacg ggcctccggg gcaaatgcga gtgcatccgc ggacgcaagg
     3181 ccaccctgga ggagctacag acggtgcact cggaagccca caccctcctg tatggcacga
     3241 accccctcaa ccggcagaaa ctggacagta agaaacttct aggctcgctc gcctccgtgt
     3301 tcgtccggct cccttgcggt ggtgttgggg tggacagtga caccatatgg aacgaggtgc
     3361 actcggcggg ggcagcccgc ctggctgtgg gctgcgtggt agagctggtc ttcaaggtgg
     3421 ccacagggga gctgaagaat ggctttgctg tggtccgccc ccctggacac catgcggagg
     3481 agagcacgcc catgggcttt tgctacttca actccgtggc cgtggcagcc aagcttctgc
     3541 agcagaggtt gagcgtgagc aagatcctca tcgtggactg ggacgtgcac catggaaacg
     3601 ggacccagca ggctttctac agcgacccca gcgtcctgta catgtccctc caccgctacg
     3661 acgatgggaa cttcttccca ggcagcgggg ctcctgatga ggtgggcaca gggcccggcg
     3721 tgggtttcaa cgtcaacatg gctttcaccg gcggcctgga cccccccatg ggagacgctg
     3781 agtacttggc ggccttcaga acggtggtca tgccgatcgc cagcgagttt gccccggatg
     3841 tggtgctggt gtcatcaggc ttcgatgccg tggagggcca ccccacccct cttgggggct
     3901 acaacctctc cgccagatgc ttcgggtacc tgacgaagca gctgatgggc ctggctggcg
     3961 gccggattgt cctggccctc gagggaggcc acgacctgac cgccatttgc gacgcctcgg
     4021 aagcatgtgt ttctgccttg ctgggaaacg agcttgatcc tctcccagaa aaggttttac
     4081 agcaaagacc caatgcaaac gctgtccgtt ccatggagaa agtcatggag atccacagca
     4141 agtactggcg ctgcctgcag cgcacaacct ccacagcggg gcgttctctg atcgaggctc
     4201 agacttgcga gaacgaagaa gccgagacgg tcaccgccat ggcctcgctg tccgtgggcg
     4261 tgaagcccgc cgaaaagaga ccagatgagg agcccatgga agaggagccg cccctggttt
     4321 ctctcggctc gagcggcgtc ttcacactcg aagatttcgt tggggactgg cgacagacag
     4381 ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc
     4441 tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga
     4501 tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg
     4561 aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact
     4621 atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt
     4681 atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg
     4741 gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa
     4801 ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa ggccgcgact
     4861 ctagagtcga cctgcaggca tgcaagctga tccggctgct aacaaagccc gaaaggaagc
     4921 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cggccgcttc
     4981 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5041 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5101 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     5161 tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc gaattttaac
     5221 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5281 tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     5341 aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     5401 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5461 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5521 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5581 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5641 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5701 cctaggcttt tgcaaaaagc ttaattaact gttgacaatt aatcatcggc atagtatatc
     5761 ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca tgattgaaca
     5821 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5881 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5941 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6001 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6061 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6121 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6181 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6241 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6301 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6361 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6421 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6481 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6541 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6601 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6661 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     6721 gatagcgata aggatcctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     6781 gacattcatc cggggtcagc accgtttctg cggactggct ttctacccgg tatcagctca
     6841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt
     7561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7621 attatcaaaa aggatcttca cctagatcct tttatagtcc ggaaatacag gaacgcacgc
     7681 tggatggccc ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag tggattaagt
     7741 gggggtaatg tggcctgtac cctctggttg cataggtatt catacggtta aaatttatca
     7801 ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg
     7861 atcgcgctga acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac
     7921 tgtctgccct cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtccggcgt
     7981 agaggatcga gatct
//
