LOCUS       Exported                6510 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC32K(pF-Nc5K3)      5119 bp    DNA   circular         
              12/7/2015

            DEFINITION  Current Name: pFC32K (pF-Nc5K3) Old/Alternate 
            Name(s):pF-Nc5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="SgfI Site"
     misc_feature    1065..2831
                     /label=PPP2R1A
                     /note="PPP2R1A"
     misc_feature    2838..2852
                     /label=C Terminal NanoLuc linker
     misc_feature    2841..2852
                     /label=N Terminal NanoLuc linker
     CDS             2853..3362
                     /label=C terminal NanoLuc
     polyA_signal    3499..3720
                     /label=Sv40 late polyadenylation signal
     promoter        3819..4237
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(3892..4128)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      4135..4200
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        4245..4311
                     /label=EM7 bacterial promoter
     CDS             4325..5119
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5183..5231
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5467..5503
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6174..6459
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggcg gcggccgacg
     1081 gcgacgactc gctgtacccc atcgcggtgc tcatagacga actccgcaat gaggacgttc
     1141 agcttcgcct caacagcatc aagaagctgt ccaccatcgc cttggccctt ggggttgaaa
     1201 ggacccgaag tgagcttctg cctttcctta cagataccat ctatgatgaa gatgaggtcc
     1261 tcctggccct ggcagaacag ctgggaacct tcactaccct ggtgggaggc ccagagtacg
     1321 tgcactgcct gctgccaccg ctggagtcgc tggccacagt ggaggagaca gtggtgcggg
     1381 acaaggcagt ggagtcctta cgggccatct cacacgagca ctcgccctct gacctggagg
     1441 cgcactttgt gccgctagtg aagcggctgg cgggcggcga ctggttcacc tcccgcacct
     1501 cggcctgcgg cctcttctcc gtctgctacc cccgagtgtc cagtgctgtg aaggcggaac
     1561 ttcgacagta cttccggaac ctgtgctcag atgacacccc catggtgcgg cgggccgcag
     1621 cctccaagct gggggagttt gccaaggtgc tggagctgga caacgtcaag agtgagatca
     1681 tccccatgtt ctccaacctg gcctctgacg agcaggactc ggtgcggctg ctggcggtgg
     1741 aggcgtgcgt gaacatcgcc cagcttctgc cccaggagga tctggaggcc ctggtgatgc
     1801 ccactctgcg ccaggccgct gaagacaagt cctggcgcgt ccgctacatg gtggctgaca
     1861 agttcacaga gctccagaaa gcagtggggc ctgagatcac caagacagac ctggtccctg
     1921 ccttccagaa cctgatgaaa gactgtgagg ccgaggtgag ggccgcagcc tcccacaagg
     1981 tcaaagagtt ctgtgaaaac ctctcagctg actgtcggga gaatgtgatc atgtcccaga
     2041 tcttgccctg catcaaggag ctggtgtccg atgccaacca acatgtcaag tctgccctgg
     2101 cctcagtcat catgggtctc tctcccatct tgggcaaaga caacaccatc gagcacctct
     2161 tgcccctctt cctggctcag ctgaaggatg agtgccctga ggtacggctg aacatcatct
     2221 ctaacctgga ctgtgtgaac gaggtgattg gcatccggca gctgtcccag tccctgctcc
     2281 ctgccattgt ggagctggct gaggacgcca agtggcgggt gcggctggcc atcattgagt
     2341 acatgcccct cctggctgga cagctgggag tggagttctt tgatgagaaa cttaactcct
     2401 tgtgcatggc ctggcttgtg gatcatgtat atgccatccg cgaggcagcc accagcaacc
     2461 tgaagaagct agtggaaaag tttgggaagg agtgggccca tgccacaatc atccccaagg
     2521 tcttggccat gtccggagac cccaactacc tgcaccgcat gactacgctc ttctgcatca
     2581 atgtgctgtc tgaggtctgt gggcaggaca tcaccaccaa gcacatgcta cccacggttc
     2641 tgcgcatggc tggggacccg gttgccaatg tccgcttcaa tgtggccaag tctctgcaga
     2701 agatagggcc catcctggac aacagcacct tgcagagtga agtcaagccc atcctagaga
     2761 agctgaccca ggaccaggat gtggacgtca aatactttgc ccaggaggct ctgactgttc
     2821 tgtctctcgc cgtttctctc ggctcgagcg gcgtcttcac actcgaagat ttcgttgggg
     2881 actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca
     2941 gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg
     3001 aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg agcggcgacc
     3061 aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat catcacttta
     3121 aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac atgatcgact
     3181 atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc actgtaacag
     3241 ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc gacggctccc
     3301 tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa cgcattctgg
     3361 cgtaaggccg cgactctaga gtcgacctgc aggcatgcaa gctgatccgg ctgctaacaa
     3421 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     3481 tggggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     3541 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3601 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3661 aggttcaggg ggagatgtgg gaggtttttt taagcaagta aaacctctac aaatgtggta
     3721 aaatcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     3781 cgcatctgtg cggtatttca caccgcatac gcggatctgc gcagcaccat ggcctgaaat
     3841 aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa
     3901 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3961 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4021 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4081 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4141 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4201 aggctttttt ggaggcctag gcttttgcaa aaagcttaat taactgttga caattaatca
     4261 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca
     4321 gatcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4381 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4441 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4501 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4561 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4621 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4681 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4741 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4801 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     4861 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4921 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4981 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5041 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5101 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5161 aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     5221 ttttttgtgt gaatcgatag cgataaggat cctctttgcg cttgcgtttt cccttgtcca
     5281 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     5341 cccggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5641 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5881 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     5941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatttcaaga
     6061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttat agtccggaaa
     6181 tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc
     6241 ttcagtggat taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac
     6301 ggttaaaatt tatcaggcgc gattgcggca gtttttcggg tggtttgttg ccatttttac
     6361 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat
     6421 ggtaaatcca cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca
     6481 cgatgcgtcc ggcgtagagg atcgagatct
//
