LOCUS       Exported                6648 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC32K(pF-Nc5K3)-SYK      6648 bp    DNA   circular     
                  2/4/2015

            DEFINITION  Current Name: pFC32K (pF-Nc5K3) - SYK
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1064..2969
                     /label=SYK
     misc_feature    2970..2976
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2977..2990
                     /label=C Terminal NanoLuc linker
     CDS             2991..3500
                     /label=C terminal NanoLuc
     polyA_signal    3637..3858
                     /label=Sv40 late polyadenylation signal
     promoter        3957..4375
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4383..4449
                     /label=EM7 bacterial promoter
     CDS             4463..5257
                     /label=Neo R / Kan R
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5321..5369
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5605..5641
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6312..6597
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggcc agcagcggca
     1081 tggctgacag cgccaaccac ctgcccttct ttttcggcaa catcacccgg gaggaggcag
     1141 aagattacct ggtccagggg ggcatgagtg atgggcttta tttgctgcgc cagagccgca
     1201 actacctggg tggcttcgcc ctgtccgtgg cccacgggag gaaggcacac cactacacca
     1261 tcgagcggga gctgaatggc acctacgcca tcgccggtgg caggacccat gccagccccg
     1321 ccgacctctg ccactaccac tcccaggagt ctgatggcct ggtctgcctc ctcaagaagc
     1381 ccttcaaccg gccccaaggg gtgcagccca agactgggcc ctttgaggat ttgaaggaaa
     1441 acctcatcag ggaatatgtg aagcagacat ggaacctgca gggtcaggct ctggagcagg
     1501 ccatcatcag tcagaagcct cagctggaga agctgatcgc taccacagcc catgaaaaaa
     1561 tgccttggtt ccatggaaaa atctctcggg aagaatctga gcaaattgtc ctgataggat
     1621 caaagacaaa tggaaagttc ctgatccgag ccagagacaa caacggctcc tacgccctgt
     1681 gcctgctgca cgaagggaag gtgctgcact atcgcatcga caaagacaag acagggaagc
     1741 tctccatccc cgagggaaag aagttcgaca cgctctggca gctagtcgag cattattctt
     1801 ataaagcaga tggtttgtta agagttctta ctgtcccatg tcaaaaaatc ggcacacagg
     1861 gaaatgttaa ttttggaggc cgtccacaac ttccaggttc ccatcctgcg acttggtcag
     1921 cgggtggaat aatctcaaga atcaaatcat actccttccc aaagcctggc cacagaaagt
     1981 cctcccctgc ccaagggaac cggcaagaga gtactgtgtc attcaatccg tatgagccag
     2041 aacttgcacc ctgggctgca gacaaaggcc cccagagaga agccctaccc atggacacag
     2101 aggtgtacga gagcccctac gcggaccccg aggagatcag gcccaaggag gtttacctgg
     2161 accgaaagct gctgacgctg gaagacaaag aactgggctc tggtaatttt ggaactgtga
     2221 aaaagggcta ctaccaaatg aaaaaagttg tgaaaaccgt ggctgtgaaa atactgaaaa
     2281 acgaggccaa tgaccccgct cttaaagatg agttattagc agaagcaaat gtcatgcagc
     2341 agctggacaa cccgtacatc gtgcggatga tcgggatatg cgaggccgag tcctggatgc
     2401 tggttatgga gatggcagaa cttggtcccc tcaataagta tttgcagcag aacagacatg
     2461 tcaaggataa gaacatcata gaactggttc atcaggtttc catgggcatg aagtacttgg
     2521 aggagagcaa ttttgtgcac agagatctgg ctgcaagaaa tgtgttgcta gttacccaac
     2581 attacgccaa gatcagtgat ttcggacttt ccaaagcact gcgtgctgat gaaaactact
     2641 acaaggccca gacccatgga aagtggcctg tcaagtggta cgctccggaa tgcatcaact
     2701 actacaagtt ctccagcaaa agcgatgtct ggagctttgg agtgttgatg tgggaagcat
     2761 tctcctatgg gcagaagcca tatcgaggga tgaaaggaag tgaagtcacc gctatgttag
     2821 agaaaggaga gcggatgggg tgccctgcag ggtgtccaag agagatgtac gatctcatga
     2881 atctgtgctg gacatacgat gtggaaaaca ggcccggatt cgcagcagtg gaactgcggc
     2941 tgcgcaatta ctactatgac gtggtgaacg tttctctcgg ctcgagcggc gtcttcacac
     3001 tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg
     3061 aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa
     3121 ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg
     3181 aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg
     3241 tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta
     3301 cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca
     3361 aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga
     3421 tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc
     3481 tgtgcgaacg cattctggcg taaggccgcg actctagagt cgacctgcag gcatgcaagc
     3541 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     3601 ataactagca taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg
     3661 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3721 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3781 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa
     3841 acctctacaa atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga
     3901 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc
     3961 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     4021 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4081 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4141 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4201 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4261 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4321 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaatta
     4381 actgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     4441 aactaaaccc aggaggcaga tcatgattga acaagatgga ttgcacgcag gttctccggc
     4501 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4561 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4621 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     4681 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4741 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4801 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4861 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4921 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4981 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5041 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5101 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5161 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5221 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5281 accgaccaag cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc
     5341 attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc tctttgcgct
     5401 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     5461 ctgcggactg gctttctacc cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5521 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6181 aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6301 ccttttatag tccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa
     6361 accatgaaaa atggcagctt cagtggatta agtgggggta atgtggcctg taccctctgg
     6421 ttgcataggt attcatacgg ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg
     6481 gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc
     6541 gtacaattaa gggattatgg taaatccact tactgtctgc cctcgtagcc atcgagataa
     6601 accgcagtac tccggccacg atgcgtccgg cgtagaggat cgagatct
//
