LOCUS       Exported                5882 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     misc_feature    805..1147
                     /label=DAP12
                     /note="DAP12"
     misc_feature    complement(1149..1156)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1161..1187
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(1188..1204)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(1207..1252)
                     /label=Minimal CMV Promoter 2
     misc_feature    1230..1252
                     /label=core
                     /note="core"
     promoter        1262..1816
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        1834..1900
                     /label=EM7 bacterial promoter
     CDS             1914..2573
                     /label=Chloramphenicol resistance
     rep_origin      2742..2778
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(3460..3507)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(3531..4325)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(4339..4633)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(4704..5882)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaactt tgtaatacgg cctctgtgtg ttgaggtcgc
      841 tgtagacatc cgacctctga ccctggagct cctgataagg cgactcggtc tcagtgatac
      901 gctgtttccg ggtcgctgcc tccgcagccc ctcgcccccg agggaccagc cggcccagga
      961 agtacacggc cagggcaatg agcactgtca gcaccaggtc tcccatcacg atccctgcca
     1021 gcacgcccgg gctcaccgta gagcaactgc aatcgctctg ggcctgggcc tggacaggac
     1081 ggagaccact tacagccagc aggagaggca ggagcaggag cctgctgcag ggttcaagtc
     1141 cccccatggc gatcgctagc tgcaaaaaga acaagtagct tgtattctat agtgtcacct
     1201 aaatgggcga tctgacggtt cactaaacca gctctgctta tataggcctc cctggcgcgc
     1261 cactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1321 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1381 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1441 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1501 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1561 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1621 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     1681 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1741 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
     1801 tgtacggtgg gaggtcaaat cgaattctaa tactgttgac aattaatcat cggcatagta
     1861 tatcggcata gtataatacg acaaggtgag gaactaaacc caggaggcag atcatggaga
     1921 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     1981 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     2041 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     2101 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     2161 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     2221 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     2281 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     2341 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     2401 tggacaactt cttcgccccc gttttcacta tgggcaaata ttatacgcaa ggcgacaagg
     2461 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     2521 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taagcgggac
     2581 tctggggttc gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg
     2641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3181 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3301 cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg
     3361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3421 aggatcttca cctagatcct tttatagtcc ggaatcgtca cacaaaaaac caacacacag
     3481 atgtaatgaa aataaagata ttttattgcg gccatcgtga tggctagagt tcagaagaac
     3541 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     3601 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     3661 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     3721 cggccatttt ccaccatgat attcggcaag caggcatcgc cgtgggtcac gacgagatcc
     3781 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     3841 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     3901 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     3961 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4021 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4081 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4141 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     4201 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     4261 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     4321 atcatggtgg catcgattcc cggtcaacat ccagtacacc tccggcacca ccggcttccc
     4381 caagggcgcc acgctctccc accacaacat cctcaacaac ggctactggg tgggccgcac
     4441 ggtcggctac acggagcagg accgggtgtg tctgccggtg cccttctacc actgcttcgg
     4501 catggtcatg ggcaacttgg gcgccacctc ccacgccgcc tgcatcgtca tcccggcccc
     4561 gtccttcgag ccggcggcga cgctggaggc ggtgcagcgg gagaggtgca cgtccctgta
     4621 cggcgtcccc acgaagccga attccaccac actggactag atgcatgctc gacagtgtga
     4681 tggatatctg cagaattcgg ctttcacgac acctgaaatg gaagaaaaaa actttgaacc
     4741 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag
     4801 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac
     4861 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac
     4921 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag
     4981 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc
     5041 gtcctccatt ttgagctgcc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca
     5101 actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg
     5161 ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc
     5221 gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc
     5281 gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct
     5341 tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg
     5401 aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca
     5461 ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc
     5521 tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa
     5581 gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac
     5641 acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact
     5701 actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca
     5761 tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct
     5821 ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag
     5881 cc
//
