LOCUS       Exported                6233 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     misc_feature    806..1498
                     /label=TREM2
                     /note="TREM2"
     misc_feature    complement(1500..1507)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1512..1538
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(1539..1555)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(1558..1603)
                     /label=Minimal CMV Promoter 2
     misc_feature    1581..1603
                     /label=core
                     /note="core"
     promoter        1613..2167
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2185..2251
                     /label=EM7 bacterial promoter
     CDS             2265..2924
                     /label=Chloramphenicol resistance
     rep_origin      3093..3129
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(3811..3858)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(3882..4676)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(4690..4984)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5055..6233)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaaccg tgtctctcag ccctggcaga gtttggagct
      841 gataccctgg gtcatggcca cagtccagtt cactgggtgg atgtgtccct ggcttctgtc
      901 catgccaggc tgcagcccag agggcgctgg ctgctagaat cttgatgaga aagatgcagg
      961 ccaggaggag aaggatggaa gtgggtggga aggggatttc tccttccaag aggctcctgg
     1021 agatgctgtg ctccacatgg gcatcctcga agctctcaga ctccccgggg aaccagagat
     1081 ctccagcatc ccggtgatcc agggggtctg ccagcacctc caccaggacc ttcctgaggg
     1141 tgtcagcctc actgccatgg aggctctggc actggtagag acccgcatca tggggttgta
     1201 gattccgcag cgtaatggtg agagtgccac ccagggtatc gtctgtgatg gctgtgctcc
     1261 cattccacct cctcaggaag gacagcagcc acaagttgtg cgtgctgacc acacgctggc
     1321 atgggccctt ctctcccagc tggcggcacc aggccttgcg cctcccccag tgcttcatgg
     1381 agtcataggg gcaagacacc tgcagggact ggcccgccac gccctggaac actgtggtgt
     1441 tgtgggctcc ggacagctct gtgacaaaga gtaagatgag cagccggaga ggctccatgg
     1501 cgatcgctag ctgcaaaaag aacaagtagc ttgtattcta tagtgtcacc taaatgggcg
     1561 atctgacggt tcactaaacc agctctgctt atataggcct ccctggcgcg ccactagtta
     1621 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     1681 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     1741 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     1801 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
     1861 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1921 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1981 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
     2041 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     2101 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
     2161 ggaggtcaaa tcgaattcta atactgttga caattaatca tcggcatagt atatcggcat
     2221 agtataatac gacaaggtga ggaactaaac ccaggaggca gatcatggag aaaaaaatca
     2281 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     2341 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     2401 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     2461 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     2521 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     2581 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2641 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     2701 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2761 tcttcgcccc cgttttcact atgggcaaat attatacgca aggcgacaag gtgctgatgc
     2821 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
     2881 atgaattaca acagtactgc gatgagtggc agggcggggc gtaagcggga ctctggggtt
     2941 cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg
     3001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3661 acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct
     3721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3781 acctagatcc ttttatagtc cggaatcgtc acacaaaaaa ccaacacaca gatgtaatga
     3841 aaataaagat attttattgc ggccatcgtg atggctagag ttcagaagaa ctcgtcaaga
     3901 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3961 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     4021 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     4081 tccaccatga tattcggcaa gcaggcatcg ccgtgggtca cgacgagatc ctcgccgtcg
     4141 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     4201 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     4261 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     4321 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     4381 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4441 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     4501 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     4561 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     4621 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatggtg
     4681 gcatcgattc ccggtcaaca tccagtacac ctccggcacc accggcttcc ccaagggcgc
     4741 cacgctctcc caccacaaca tcctcaacaa cggctactgg gtgggccgca cggtcggcta
     4801 cacggagcag gaccgggtgt gtctgccggt gcccttctac cactgcttcg gcatggtcat
     4861 gggcaacttg ggcgccacct cccacgccgc ctgcatcgtc atcccggccc cgtccttcga
     4921 gccggcggcg acgctggagg cggtgcagcg ggagaggtgc acgtccctgt acggcgtccc
     4981 cacgaagccg aattccacca cactggacta gatgcatgct cgacagtgtg atggatatct
     5041 gcagaattcg gctttcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
     5101 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
     5161 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
     5221 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
     5281 tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag
     5341 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
     5401 tttgagctgc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
     5461 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
     5521 agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
     5581 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
     5641 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
     5701 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
     5761 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
     5821 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
     5881 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
     5941 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
     6001 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     6061 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     6121 cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac
     6181 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcc
//
