LOCUS       Exported                6951 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC27K(pFC27K)      5527 bp    DNA   circular           
            3/27/2014

            DEFINITION  Current Name: pFC27K (pFC27K) Old/Alternate 
            Name(s):pFC27K (backbone)
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..2864
                     /label=DCAF15(co)
                     /note="DCAF15(co)"
     misc_feature    2871..2915
                     /label=HT7 Linker C terminal
     misc_feature    2916..3806
                     /label=HT7 tag C terminal
     polyA_signal    3940..4161
                     /label=Sv40 late polyadenylation signal
     promoter        4260..4678
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4686..4752
                     /label=EM7 bacterial promoter
     CDS             4766..5560
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5624..5672
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5908..5944
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggct ccatcaagca
     1081 aaagcgaaag gaattccggc gcgggctccg gtggaggagg tcccggcgga gctggaggaa
     1141 agcgagccgc tggtagaagg cgagaacacg tacttaaaca gcttgaacga gttaagattt
     1201 ccgggcaact ctcaccgcgg ttgtttagga aactgccccc tagagtatgt gtgtccttga
     1261 agaacattgt ggacgaagat ttcctttacg ctgggcacat ctttctcggc ttttcaaaat
     1321 gtgggagata cgtgctttca tacaccagca gttcaggcga tgacgacttc tcattctata
     1381 tctaccattt gtattggtgg gagttcaatg tacattctaa gcttaaactt gtgcgacagg
     1441 tgaggctgtt tcaggatgag gagatatatt ccgaccttta tttgacagtt tgcgagtggc
     1501 cgtctgatgc ttccaaagtg atagtttttg gatttaatac tcggagcgcc aacggaatgc
     1561 ttatgaacat gatgatgatg tctgatgaaa atcatcgaga catttatgtc tcaacagtcg
     1621 ctgttccacc gcctggacgc tgcgctgcat gccaggacgc gagcagggcc caccccggtg
     1681 atccaaacgc ccaatgtctt cgccacgggt ttatgttgca tacgaagtac caggtcgtgt
     1741 atcccttccc cactttccaa cccgcgttcc aactgaaaaa agatcaagtc gttctgttga
     1801 acacgtcata tagtttggtt gcttgtgcgg tctctgttca tagtgcggga gacaggtctt
     1861 tctgtcaaat cctgtatgat cactccacct gtcctctcgc gccagcgtcc cctcccgagc
     1921 cacagagccc cgaactgcct ccagcacttc cttccttctg tcctgaggct gctccagccc
     1981 gctcaagcgg tagcccggag ccgtcccccg ctatcgcgaa ggcaaaagaa tttgttgcgg
     2041 acatcttcag gcgggctaag gaagcaaagg ggggtgttcc cgaggaggcc aggcccgcgc
     2101 tgtgtcctgg gccttctggg agtcgatgcc gcgcgcatag tgaaccactg gcactctgtg
     2161 gggagaccgc cccacgcgac tcacccccag cgtctgaggc accggcgtca gaaccgggct
     2221 atgtcaatta cactaaactt tattacgttc ttgaaagtgg agaggggaca gagcccgaag
     2281 acgaacttga ggatgacaag atcagcctgc cgtttgtagt gacggacttg cgcgggcgaa
     2341 atttgcggcc tatgagggaa cgaaccgccg tccaagggca gtaccttact gttgaacagc
     2401 tcactttgga ctttgagtat gtaataaacg aggtaattag acatgacgct acatggggcc
     2461 atcagttctg ctcattcagt gattatgata ttgtaatact ggaagtgtgc cctgaaacaa
     2521 atcaagtcct tataaatatc gggctgttgt tgctcgcgtt tccgagtcct actgaagaag
     2581 ggcagcttcg accgaagacc taccatacgt ctcttaaagt cgcatgggac ctcaacaccg
     2641 gcatcttcga gaccgtgagc gtgggcgatt tgacagaagt gaaaggacaa acaagcggtt
     2701 ctgtttggtc ctcctatcgg aaaagttgcg tagacatggt catgaagtgg ttggttccag
     2761 agagtagcgg tagatatgtg aatcggatga ccaatgaggc tttgcataag ggctgctctt
     2821 tgaaagtgct ggcagacagc gagcgatata cttggatagt cctggtttct ctcgagccaa
     2881 ccactgagga tctgtacttt cagagcgata acgatggatc cgaaatcggt actggctttc
     2941 cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc
     3001 cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc
     3061 gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac ctgatcggta
     3121 tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc cgcttcatgg
     3181 atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac gactggggct
     3241 ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt attgcattta
     3301 tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc cgcgagacct
     3361 tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag aacgttttta
     3421 tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt
     3481 accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc ccaaacgagc
     3541 tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac atggactggc
     3601 tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt ctgatcccac
     3661 cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg gacatcggcc
     3721 cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag atcgcgcgct
     3781 ggctgtctac tctggagatt tccggttaat agaattctag agtcgacctg caggcatgca
     3841 agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     3901 gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg
     3961 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4021 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4081 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt
     4141 aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc
     4201 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg
     4261 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     4321 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4381 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4441 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4501 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4561 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4621 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttaa
     4681 ttaactgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     4741 aggaactaaa cccaggaggc agatcatgat tgaacaagat ggattgcacg caggttctcc
     4801 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4861 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4921 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4981 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5041 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5101 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5161 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5221 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5281 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5341 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5401 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5461 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5521 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5581 atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt
     5641 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tcctctttgc
     5701 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     5761 tttctgcgga ctggctttct acccggtatc agctcactca aaggcggtaa tacggttatc
     5821 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5881 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5941 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6001 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6061 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6121 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6181 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6241 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6301 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6361 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6421 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6481 cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6541 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6601 gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     6661 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     6721 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc agtttttcgg
     6781 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     6841 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     6901 taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc t
//
