LOCUS       Exported                7973 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN28A(pF-CHnN)      6532 bp    DNA   circular          
             9/9/2014

            DEFINITION  Current Name: pFN28A (pF-CHnN) Old/Alternate 
            Name(s):pF-CHnN (backbone)
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1067..1957
                     /label=HaloTag
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    1958..1996
                     /label=HT7 Linker N terminal
     misc_feature    1970..1990
                     /label=TEV protease recognition sequence
     misc_feature    1997..2004
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2006..3805
                     /label=DCAF15(co)
                     /note="DCAF15(co)"
     misc_feature    3806..3813
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3965..4186
                     /label=Sv40 late polyadenylation signal
     promoter        4285..4703
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     CDS             4748..5542
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5606..5654
                     /label=Synthetic PolyAdenylation Signal
     CDS             5915..6775
                     /label=AmpR
                     /note="Removed RE site from standard beta-lactamase gene"
     rep_origin      6930..6966
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgccatggc tccatcaagc aaaagcgaaa ggaattccgg
     2041 cgcgggctcc ggtggaggag gtcccggcgg agctggagga aagcgagccg ctggtagaag
     2101 gcgagaacac gtacttaaac agcttgaacg agttaagatt tccgggcaac tctcaccgcg
     2161 gttgtttagg aaactgcccc ctagagtatg tgtgtccttg aagaacattg tggacgaaga
     2221 tttcctttac gctgggcaca tctttctcgg cttttcaaaa tgtgggagat acgtgctttc
     2281 atacaccagc agttcaggcg atgacgactt ctcattctat atctaccatt tgtattggtg
     2341 ggagttcaat gtacattcta agcttaaact tgtgcgacag gtgaggctgt ttcaggatga
     2401 ggagatatat tccgaccttt atttgacagt ttgcgagtgg ccgtctgatg cttccaaagt
     2461 gatagttttt ggatttaata ctcggagcgc caacggaatg cttatgaaca tgatgatgat
     2521 gtctgatgaa aatcatcgag acatttatgt ctcaacagtc gctgttccac cgcctggacg
     2581 ctgcgctgca tgccaggacg cgagcagggc ccaccccggt gatccaaacg cccaatgtct
     2641 tcgccacggg tttatgttgc atacgaagta ccaggtcgtg tatcccttcc ccactttcca
     2701 acccgcgttc caactgaaaa aagatcaagt cgttctgttg aacacgtcat atagtttggt
     2761 tgcttgtgcg gtctctgttc atagtgcggg agacaggtct ttctgtcaaa tcctgtatga
     2821 tcactccacc tgtcctctcg cgccagcgtc ccctcccgag ccacagagcc ccgaactgcc
     2881 tccagcactt ccttccttct gtcctgaggc tgctccagcc cgctcaagcg gtagcccgga
     2941 gccgtccccc gctatcgcga aggcaaaaga atttgttgcg gacatcttca ggcgggctaa
     3001 ggaagcaaag gggggtgttc ccgaggaggc caggcccgcg ctgtgtcctg ggccttctgg
     3061 gagtcgatgc cgcgcgcata gtgaaccact ggcactctgt ggggagaccg ccccacgcga
     3121 ctcaccccca gcgtctgagg caccggcgtc agaaccgggc tatgtcaatt acactaaact
     3181 ttattacgtt cttgaaagtg gagaggggac agagcccgaa gacgaacttg aggatgacaa
     3241 gatcagcctg ccgtttgtag tgacggactt gcgcgggcga aatttgcggc ctatgaggga
     3301 acgaaccgcc gtccaagggc agtaccttac tgttgaacag ctcactttgg actttgagta
     3361 tgtaataaac gaggtaatta gacatgacgc tacatggggc catcagttct gctcattcag
     3421 tgattatgat attgtaatac tggaagtgtg ccctgaaaca aatcaagtcc ttataaatat
     3481 cgggctgttg ttgctcgcgt ttccgagtcc tactgaagaa gggcagcttc gaccgaagac
     3541 ctaccatacg tctcttaaag tcgcatggga cctcaacacc ggcatcttcg agaccgtgag
     3601 cgtgggcgat ttgacagaag tgaaaggaca aacaagcggt tctgtttggt cctcctatcg
     3661 gaaaagttgc gtagacatgg tcatgaagtg gttggttcca gagagtagcg gtagatatgt
     3721 gaatcggatg accaatgagg ctttgcataa gggctgctct ttgaaagtgc tggcagacag
     3781 cgagcgatat acttggatag tcctggttta aacgaattcg ggctcggtac ccggggatcc
     3841 tctagagtcg acctgcaggc atgcaagctg atccggctgc taacaaagcc cgaaaggaag
     3901 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcggccgctt
     3961 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4021 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4081 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4141 atgtgggagg tttttttaag caagtaaaac ctctacaaat gtggtaaaat cgaattttaa
     4201 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     4261 atttcacacc gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag
     4321 gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     4381 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4441 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4501 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4561 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4621 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4681 gcctaggctt ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag
     4741 agccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4801 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4861 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4921 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4981 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5041 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5101 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5161 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5221 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5281 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5341 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5401 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5461 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5521 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5581 cccaacctgc catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     5641 tggttttttg tgtgaatcga tagcgataag gatcctcttt gcgcttgcgt tttcccttgt
     5701 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     5761 ctacgtaatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5881 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5941 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6001 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6061 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6121 tttcaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6181 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6241 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6301 taacactgcg gccaacttac ttctgacaac tatcggagga ccgaaggagc taaccgcttt
     6361 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6421 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6481 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6541 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6601 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6661 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6721 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaattcga
     6781 aatgaccgac caagcgacgc ccaaccggta tcagctcact caaaggcggt aatacggtta
     6841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     7381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7501 cgcagaaaaa aaggatttca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7621 tagatccttt tatagtccgg aaatacagga acgcacgctg gatggccctt cgctgggatg
     7681 gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc
     7741 tctggttgca taggtattca tacggttaaa atttatcagg cgcgattgcg gcagtttttc
     7801 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc
     7861 ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga
     7921 gataaaccgc agtactccgg ccacgatgcg tccggcgtag aggatcgaga tct
//
