LOCUS       Exported                6520 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN21A(pF-47nA)-BTRC      6520 bp    DNA   circular     
                  11/29/2017

            DEFINITION  Current Name: pFN21A (pF-47nA) - BTRC Old/Alternate 
            Name(s):AB587367 (insert) beta-transducin repeat containing (insert)
            beta-transducin repeat containing E3 ubiquitin pro (insert) betaTrCP
            (insert) BETA-TRCP (insert) beta-TrCP1 (insert) BTRC (insert) bTrCP 
            (insert) bTrCP1 (insert) E3RSIkappaB (insert) epididymis tissue 
            protein Li 2a (insert) F-box and WD repeats protein beta-TrCP 
            (insert) F-box and WD-repeat protein 1B (insert) F-box/WD 
            repeat-containing protein 1A (insert) FBW1A (insert) FBXW1 (insert) 
            FBXW1A (insert) FWD1 (insert) FXC11937 (insert) MGC4643 (insert) 
            pIkappaBalpha-E3 receptor subunit (insert) pF-47nA (backbone)
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1067..3826
                     /label=Complete ORF
                     /note="Recieved from Kazusa in April 2010. Entered into 
                     database semi automatically by Ethan in May 2010. Accession
                     number NM_033637 added 11/5/2010 from BLAST and length 
                     similarity. (note added in Automated Blaster by 11/5/2010 
                     1:20:33 PM"
     misc_feature    1067..1957
                     /label=HT7
     misc_feature    1958..1996
                     /label=HT7 Linker N terminal
     misc_feature    1970..1990
                     /label=TEV protease recognition sequence
     misc_feature    1997..2004
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2001..3824
                     /label=BTRC insert
                     /note="Recieved from Kazusa in April 2010. Entered into 
                     database semi automatically by Ethan in May 2010. Accession
                     number NM_033637 added 11/5/2010 from BLAST and length 
                     similarity. (note added in Automated Blaster by 11/5/2010 
                     1:20:33 PM"
     misc_feature    3821..3828
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3825..3958
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    3980..4201
                     /label=Sv40 late polyadenylation signal
     CDS             4462..5322
                     /label=beta-lactamase
     CDS             4462..5322
                     /label=Beta-lactamase
                     /note="Amp resistance gene used in some pGL4 vectors"
     rep_origin      5477..5513
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6184..6469
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgccatgga cccggccgag gcggtgctgc aagagaaggc
     2041 actcaagttt atgtgctcta tgcccaggtc tctgtggctg ggctgctcca gcctggcgga
     2101 cagcatgcct tcgctgcgat gcctgtataa cccagggact ggcgcactca cagctttcca
     2161 gaattcctca gagagagaag actgtaataa tggcgaaccc cctaggaaga taataccaga
     2221 gaagaattca cttagacaga catacaacag ctgtgccaga ctctgcttaa accaagaaac
     2281 agtatgttta gcaagcactg ctatgaagac tgagaattgt gtggccaaaa caaaacttgc
     2341 caatggcact tccagtatga ttgtgcccaa gcaacggaaa ctctcagcaa gctatgaaaa
     2401 ggaaaaggaa ctgtgtgtca aatactttga gcagtggtca gagtcagatc aagtggaatt
     2461 tgtggaacat cttatatccc aaatgtgtca ttaccaacat gggcacataa actcgtatct
     2521 taaacctatg ttgcagagag atttcataac tgctctgcca gctcggggat tggatcatat
     2581 tgctgagaac attctgtcat acctggatgc caaatcacta tgtgctgctg aacttgtgtg
     2641 caaggaatgg taccgagtga cctctgatgg catgctgtgg aagaagctta tcgagagaat
     2701 ggtcaggaca gattctctgt ggagaggcct ggcagaacga agaggatggg gacagtattt
     2761 attcaaaaac aaacctcctg acgggaatgc tcctcccaac tctttttata gagcacttta
     2821 tcctaaaatt atacaagaca ttgagacaat agaatctaat tggagatgtg gaagacatag
     2881 tttacagaga attcactgcc gaagtgaaac aagcaaagga gtttactgtt tacagtatga
     2941 tgatcagaaa atagtaagcg gccttcgaga caacacaatc aagatctggg ataaaaacac
     3001 attggaatgc aagcgaattc tcacaggcca tacaggttca gtcctctgtc tccagtatga
     3061 tgagagagtg atcataacag gatcatcgga ttccacggtc agagtgtggg atgtaaatac
     3121 aggtgaaatg ctaaacacgt tgattcacca ttgtgaagca gttctgcact tgcgtttcaa
     3181 taatggcatg atggtgacct gctccaaaga tcgttccatt gctgtatggg atatggcctc
     3241 cccaactgac attaccctcc ggagggtgct ggtcggacac cgagctgctg tcaatgttgt
     3301 agactttgat gacaagtaca ttgtttctgc atctggggat agaactataa aggtatggaa
     3361 cacaagtact tgtgaatttg taaggacctt aaatggacac aaacgaggca ttgcctgttt
     3421 gcagtacagg gacaggctgg tagtgagtgg ctcatctgac aacactatca gattatggga
     3481 catagaatgt ggtgcatgtt tacgagtgtt agaaggccat gaggaattgg tgcgttgtat
     3541 tcgatttgat aacaagagga tagtcagtgg ggcctatgat ggaaaaatta aagtgtggga
     3601 tcttgtggct gctttggacc cccgtgctcc tgcagggaca ctctgtctac ggacccttgt
     3661 ggagcattcc ggaagagttt ttcgactaca gtttgatgaa ttccagattg tcagtagttc
     3721 acatgatgac acaatcctca tctgggactt cctaaatgat ccagctgccc aagctgaacc
     3781 cccccgttcc ccttctcgaa catacaccta catctccaga gtttaaacga attcgggctc
     3841 ggtacccggg gatcctctag agtcgacctg caggcatgca agctgatccg gctgctaaca
     3901 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     3961 ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     4021 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4081 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4141 caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt
     4201 aaaatcgaat tctaatggat cctctttgcg cttgcgtttt cccttgtcca gatagcccag
     4261 tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtaatggtt
     4321 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4381 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4441 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4501 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4561 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4621 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt caaagttctg
     4681 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4741 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4801 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4861 aacttacttc tgacaactat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4921 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4981 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5041 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5101 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5161 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5221 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5281 cagatcgctg agataggtgc ctcactgatt aagcattggt aattcgaaat gaccgaccaa
     5341 gcgacgccca accggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5881 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     5941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6061 gatttcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttat
     6181 agtccggaaa tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa
     6241 aaatggcagc ttcagtggat taagtggggg taatgtggcc tgtaccctct ggttgcatag
     6301 gtattcatac ggttaaaatt tatcaggcgc gattgcggca gtttttcggg tggtttgttg
     6361 ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt
     6421 aagggattat ggtaaatcca cttactgtct gccctcgtag ccatcgagat aaaccgcagt
     6481 actccggcca cgatgcgtcc ggcgtagagg atcgagatct
//
