LOCUS       Exported                5490 bp ds-DNA     linear   SYN 30-MAY-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..2241
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..2234)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..2234
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     promoter        1..591
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          706..838
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        882..901
                     /label=T7 promoter
     misc_feature    905..912
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    914..1253
                     /label=TCEB1 (elongin C)
                     /note="TCEB1 (elongin C) transcript variant 1"
     misc_feature    1250..1253
                     /label=PmeI
                     /note="PmeI"
     misc_feature    1254..1256
                     /label=EcoICR I
     misc_feature    1256..1300
                     /label=HT7 Linker C terminal
     misc_feature    1301..2191
                     /label=HT7 tag C terminal
     misc_feature    2212..2217
                     /label=SbfI
                     /note="SbfI"
     misc_feature    2218..4386
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     polyA_signal    2269..2490
                     /label=Sv40 late polyadenylation signal
     promoter        2556..2622
                     /label=EM7 bacterial promoter
     CDS             2636..3430
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    3437..3474
                     /label=Untitled
     rep_origin      3585..3621
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4303..4350)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(4352..4386)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    4362..5215
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    4362..4396
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(4374..4772)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(4797..5215)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(5190..5215)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    5207..5215
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    5216..5482
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    5216
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(5237..5424)
                     /label=SV40\polyA\signal
     misc_feature    complement(5428..5484)
                     /label=MCS 2
     misc_feature    complement(5485..5490)
                     /label=MCS 2
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat cactagaagc
      601 tttattgcgg tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca
      661 gtctcgaact taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
      721 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
      781 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
      841 gtccactccc agttcaatta cagctcttaa ggctagagta ttaatacgac tcactatagg
      901 gctagcgatc gccatggatg gagaggagaa aacctatggt ggctgtgaag gacctgatgc
      961 catgtatgtc aaattgatat catctgatgg ccatgaattt attgtaaaaa gagaacatgc
     1021 attaacatca ggcacgataa aagccatgtt gagtggccca ggtcagtttg ctgagaacga
     1081 aaccaatgag gtcaatttta gagagatacc ttcacatgtg ctatcgaaag tatgcatgta
     1141 ttttacgtac aaggttcgct acactaacag ctccaccgag attcctgaat tcccaattgc
     1201 acctgaaatt gcactggaac tgctgatggc tgcgaacttc ttagattgtg tttctctcga
     1261 gccaaccact gaggatctgt actttcagag cgataacgat ggatccgaaa tcggtactgg
     1321 ctttccattc gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt
     1381 tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt
     1441 gtggcgcaac atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat
     1501 cggtatgggc aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt
     1561 catggatgcc ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg
     1621 gggctccgct ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc
     1681 atttatggag ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga
     1741 gaccttccag gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt
     1801 ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga
     1861 ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa
     1921 cgagctgcca atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga
     1981 ctggctgcac cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat
     2041 cccaccggcc gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat
     2101 cggcccgggt ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc
     2161 gcgctggctg tctactctgg agatttccgg ttaatagaat tctagagtcg acctgcaggc
     2221 atgcaagctg atttggctgc tgccaccgct gagcaaggcc gcttcgagca gacatgataa
     2281 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2341 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2401 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     2461 taagcaagta aaacctctac aaatgtggta aaatcttaat taagaattct aatataactt
     2521 cgtatagcat acattatacg aagttatgga tccactgttg acaattaatc atcggcatag
     2581 tatatcggca tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat
     2641 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     2701 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     2761 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     2821 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     2881 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     2941 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3001 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3061 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3121 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     3181 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     3241 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     3301 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     3361 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     3421 gttcttctga ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag
     3481 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4021 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4141 aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg
     4201 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4261 aaaaggatct tcacctagat ccttttatag tccggaatcg tcacacaaaa aaccaacaca
     4321 cagatgtaat gaaaataaag atattttatt gcggccatcg tgatggctag agtttagccc
     4381 tcccacacat aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca
     4441 ctgtccttca ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt
     4501 ccgcaggggc tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg
     4561 ccagctgccg cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta
     4621 aaatgatata cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg
     4681 acgctgtagt cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg
     4741 gtggattctt cttgagacaa aggcttggcc atggtggcgc tagtgtcaga agaatcgagc
     4801 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     4861 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     4921 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     4981 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     5041 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     5101 cctggggact ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag
     5161 aaggtaccta accaagttcc tctttcagag gttatttcag gccatggtgc tgcgcaagac
     5221 gcgttaatta agacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     5281 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     5341 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     5401 caaatgtggt atggctgatt atgatccgcg gccgcaatac gtcgacgtta tcagctgact
     5461 tcgtacgaga gcctaggatt atggcgcgcc
//
