LOCUS       Exported                5065 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC14K(pF-CHcK)      5051 bp    DNA   circular          
             11/15/2012

            DEFINITION  Current Name: pFC14K (pF-CHcK) Old/Alternate 
            Name(s):pF-CHcK (backbone)
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..1454
                     /label=H2A
                     /note="H2A"
     misc_feature    1461..1505
                     /label=HT7 Linker C terminal
     misc_feature    1476..1496
                     /label=TEV protease recognition sequence
     misc_feature    1506..2396
                     /label=HaloTag
     polyA_signal    2530..2751
                     /label=Sv40 late polyadenylation signal
     CDS             3059..3853
                     /label=KanR
     rep_origin      4022..4058
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgtcc ggtcgtggta
     1081 agcagggtgg caaggcgcgc gccaaggcta agtcgcgctc gtcgcgcgcg gggctgcagt
     1141 tccccgtggg ccgcgtgcac cggttgctcc gcaagggcaa ctattcggag cgcgtgggcg
     1201 ccggcgcccc ggtctatctg gccgcggtgc tcgagtactt gactgccgag atcctggagc
     1261 ttgccggcaa cgcggcgcgc gacaacaaga agacgcgcat catcccgcgc cacctgcagc
     1321 tggccatccg caacgacgag gagctcaaca agctgctggg ccgcgtgacc atcgcgcagg
     1381 gtggcgtcct gcccaacatc caggccgtac tgctgcccaa gaagacggag agccaccaca
     1441 aggccaaggg caaggtttct ctcgagccaa ccactgagga tctgtacttt cagagcgata
     1501 acgatggatc cgaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg
     1561 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc
     1621 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc
     1681 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt
     1741 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag
     1801 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca
     1861 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg
     1921 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc
     1981 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc
     2041 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc
     2101 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg
     2161 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt
     2221 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc
     2281 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc
     2341 cggacctgat cggcagcgag atcgcgcgct ggctgtctac tctggagatt tccggttaat
     2401 agaattctag agtcgacctg caggcatgca agctgatccg gctgctaaca aagcccgaaa
     2461 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc
     2521 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2581 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2641 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2701 gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat
     2761 tctaatggat cctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     2821 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     2881 agcccttgcg ccctgagtgc ttgcggcagc gtgagcttca aaagaattgc cagctggggc
     2941 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     3001 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgacggt cgtttcgcat
     3061 gcttgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3121 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3181 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3241 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     3301 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     3361 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     3421 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     3481 cgagcgagca cgcactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     3541 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgta tgccggatgg
     3601 tgaggatctc gtcgtgactc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     3661 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     3721 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     3781 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     3841 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaaccgg
     3901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4441 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     4501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt caagaagatc
     4621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttatagtcc ggaaatacag
     4741 gaacgcacgc tggatggccc ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag
     4801 tggattaagt gggggtaatg tggcctgtac cctctggttg cataggtatt catacggtta
     4861 aaatttatca ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt tttacctgtc
     4921 tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta caattaaggg attatggtaa
     4981 atccacttac tgtctgccct cgtagccatc gagataaacc gcagtactcc ggccacgatg
     5041 cgtccggcgt agaggatcga gatct
//
