LOCUS       Exported                7912 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    301..2643
                     /label=beta-catenin
                     /note="beta-catenin"
     misc_feature    complement(2645..2652)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(2653..2664)
                     /label=linker
                     /note="linker"
     misc_feature    complement(2665..3177)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    3191..3217
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(3218..3234)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(3237..3282)
                     /label=Minimal CMV Promoter 2
     misc_feature    3260..3282
                     /label=core
                     /note="core"
     promoter        3292..3846
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        3864..3930
                     /label=EM7 bacterial promoter
     CDS             3944..4603
                     /label=Chloramphenicol resistance
     rep_origin      4772..4808
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5490..5537)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(5561..6355)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(6369..6663)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(6734..7912)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 caggtcagta tcaaaccagg ccagctgatt gctgtcacct ggaggcagcc catccatgag
      361 gtcctgggca tgccccagat ctggcagccc atcaactgga tagtcagcac cagggtggtg
      421 gccacccatc tcatgttcca tcatggggtc catacccaag gcatcctggc catatccacc
      481 agagtgaaaa gaacgatagc taggatcatc ctggcgatat ccaaggggtt ctccctgggc
      541 accaatatca agtccaagat cagcagtctc attccaagcc attggctctg ttctgaagag
      601 agagctggtc agctcaactg aaagccgttt cttgtaatct tgtggcttgt cctcagacat
      661 tcggaacaaa acagcagctg catatgtcgc cacaccttca ttcctagagt gaagtaactc
      721 tgtcagagga gctgtggctc cctcagcttc aatagcttct gcagcttcct tgtcctgagc
      781 aagttcacag aggacccctg cagctactct ttggatgttt tcaatgggag aataaagcag
      841 ctgcacaaac aatggaatgg tatttagtcc tctgataaca attcggttgt gaacatcccg
      901 agctaggatg tgaagggctc cggtacaacc ttcaactatt tcttccatgc ggaccccctc
      961 cacaaattgc tgctgtgtcc cacccatgga cgtacggcgc tgggtatcct gatgtgcacg
     1021 aacaagcaac tgaactagtc gtggaatggc accctgctca cgcaaaggtg catgatttgc
     1081 gggacaaagg gcaagatttc gaatcaatcc aacagtagcc tttatcagag gccagtggga
     1141 tggtgggtgt aagagcttaa ccacaactgg tagtccatag tgaaggcgaa ctgcattctg
     1201 ggccatctct gcttcttggt gtcggctggt cagatgacga agagcacaga tggcaggctc
     1261 agtgatgtct tccctgtcac cagcccgaag gacagtacgc acaagagcct ctataccacc
     1321 cacttggcag accatcatct tgttcttata attattgcaa gtgaggttag aaagaattcc
     1381 agctgcacag gtgaccacat ttatatcatc tgaacccaga agctgaacaa gagtcccaag
     1441 gagaccttcc atcccttcct gtttagttgc agcatctgaa agattcctga gagtccaaag
     1501 acagttctga acaagacgtt gacttggatc tgtcaggtga agtcctaaag cttgcattcc
     1561 accagcttct acaatagccg gcttattact agagcagaca gatagcacct tcagcactct
     1621 gcttgtggtc cacagtagtt tttcgtaagt ataggtcctc attatattta ctaaagcttg
     1681 gggtccacca ctagccagta tgatgagctt gctttcttgg ttgccataag ctaaaatttg
     1741 aaggcagtct gtcgtaatag ccaagaattt aacatttgtt ttgttgagca aggcaaccat
     1801 tttctgcagc ccaccagcta aacgcactgc cattttagct ccttcttgat gtaataaaag
     1861 gttgtggaga gttgtaatgg cataaaacaa cacagaatcc actggtgaac caagcatttt
     1921 caccagggca ggaatgcctc cagacttaaa gatggccagt aagccctcac gatgatggga
     1981 aaggttatgc aaggtcccag cggtacaacg agctgtttct acatcatttg tattctgcat
     2041 ggtacgtaca atagcagaca ccatctgagg agaacgcatg atagcgtgtc tggaagcttc
     2101 ctttttagaa agctgatgga ccataactgc agccttatta accaccacct ggtcctcgtc
     2161 atttagcagt tttgtcagtt cagggattgc acgtgtggca agttctgcat catcttgata
     2221 gttaatcaag tttacaactg catgtttcag catctgtgat ggttcagcca aacgctggac
     2281 attagtggga tgagcagcat caaactgtgt agatgggatc tgcatgccct catctaatgt
     2341 ctcagggaac atagcagctc gtaccctctg agctcgagtc attgcatact gtccatcaat
     2401 atcagctact tgttcttgag tgaaggactg agaaaatccc tgttcccact catacaggac
     2461 ttgggaggta tccacatcct cttcctcagg attgccttta ccactcagag aaggagctgt
     2521 ggtagtggca ccagaatgga ttccagagtc caggtaagac tgttgctgcc agtgactaac
     2581 agccgctttt ctgtctggtt ccatggccat gtccaactcc atcaaatcag cttgagtagc
     2641 catggcgatc gcgccgctcg agcccgccag aatgcgttcg cacagccgcc agccggtcac
     2701 tccgttgatg gttactcgga acagcaggga gccgtcgggg ttgatcaggc gctcgtcgat
     2761 aattttgttg ccgttccaca gggtccctgt tacagtgatc tttttgccgt cgaacacggc
     2821 gatgccttca tacggccgtc cgaaatagtc gatcatgttc ggcgtaaccc cgtcgattac
     2881 cagtgtgcca tagtgcagga tcaccttaaa gtgatgatca tccacagggt acaccacctt
     2941 aaaaattttt tcgatctggc ccatttggtc gccgctcaga ccttcatacg ggatgatgac
     3001 atggatgtcg atcttcagcc cattttcacc gctcaggaca atcctttgga tcggagttac
     3061 ggacaccccg agattctgaa acaaactgga cacacctccc tgttcaagga cttggtccag
     3121 gttgtagccg gctgtctgtc gccagtcccc aacgaaatct tcgagtgtga agaccatggt
     3181 gagcgctagc tgcaaaaaga acaagtagct tgtattctat agtgtcacct aaatgggcga
     3241 tctgacggtt cactaaacca gctctgctta tataggcctc cctggcgcgc cactagttat
     3301 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3361 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3421 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3481 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     3541 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3601 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3661 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3721 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3781 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
     3841 gaggtcaaat cgaattctaa tactgttgac aattaatcat cggcatagta tatcggcata
     3901 gtataatacg acaaggtgag gaactaaacc caggaggcag atcatggaga aaaaaatcac
     3961 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     4021 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     4081 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     4141 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     4201 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     4261 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     4321 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     4381 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     4441 cttcgccccc gttttcacta tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     4501 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     4561 tgaattacaa cagtactgcg atgagtggca gggcggggcg taagcgggac tctggggttc
     4621 gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt
     4681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     5221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5341 cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5401 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5461 cctagatcct tttatagtcc ggaatcgtca cacaaaaaac caacacacag atgtaatgaa
     5521 aataaagata ttttattgcg gccatcgtga tggctagagt tcagaagaac tcgtcaagaa
     5581 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     5641 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     5701 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     5761 ccaccatgat attcggcaag caggcatcgc cgtgggtcac gacgagatcc tcgccgtcgg
     5821 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     5881 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     5941 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     6001 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     6061 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     6121 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     6181 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     6241 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     6301 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatggtgg
     6361 catcgattcc cggtcaacat ccagtacacc tccggcacca ccggcttccc caagggcgcc
     6421 acgctctccc accacaacat cctcaacaac ggctactggg tgggccgcac ggtcggctac
     6481 acggagcagg accgggtgtg tctgccggtg cccttctacc actgcttcgg catggtcatg
     6541 ggcaacttgg gcgccacctc ccacgccgcc tgcatcgtca tcccggcccc gtccttcgag
     6601 ccggcggcga cgctggaggc ggtgcagcgg gagaggtgca cgtccctgta cggcgtcccc
     6661 acgaagccga attccaccac actggactag atgcatgctc gacagtgtga tggatatctg
     6721 cagaattcgg ctttcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag
     6781 gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca
     6841 agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat
     6901 aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt
     6961 gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg
     7021 cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt
     7081 ttgagctgcc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg
     7141 accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca
     7201 gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc
     7261 cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa
     7321 aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa
     7381 aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga
     7441 gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct
     7501 caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac
     7561 ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa gtaattcaag
     7621 gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta
     7681 cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta
     7741 tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc
     7801 agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact
     7861 tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cc
//
