LOCUS       Exported                6869 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     misc_feature    complement(805..808)
                     /label=PmeI
                     /note="PmeI"
     misc_feature    complement(809..2134)
                     /label=CRBN
                     /note="CRBN"
     misc_feature    complement(2136..2143)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2148..2174
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2175..2191)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2194..2239)
                     /label=Minimal CMV Promoter 2
     misc_feature    2217..2239
                     /label=core
                     /note="core"
     promoter        2249..2803
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2821..2887
                     /label=EM7 bacterial promoter
     CDS             2901..3560
                     /label=Chloramphenicol resistance
     rep_origin      3729..3765
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4447..4494)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4518..5312)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5326..5620)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5691..6869)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacca agcaaagtat tactttgtct ggacttattt
      841 catcttcagt gtctgggatc gtgggcaaca gagcagatcg cgttaagccc caaaattttt
      901 gaggtgacat gtcttttttg gtggccgtaa acttccatcc aatatggctt gcacagatct
      961 tacactgggc aacagtccag gcatacccag gaaaccagct gtgttctgta gaaggccggc
     1021 ctatcagatt caagttgcaa gccttataca cagtaagtgt ctcatgcaca tatccatgag
     1081 gattcacata agctgccatc ggcccacata aggataaact gaatatttca tttttggttg
     1141 ttatttctgt ttcttgacat tgtttacagc aaagggaagt acatttattc ataatgtcta
     1201 attcacagcg aagtcgctgg atagcactgc caattttaag gagctgaatt ctcaatacat
     1261 catcaatagg aagacaagca gctactctgt aagaaaaatc tattggattt gaaggaagag
     1321 aatcatcttt tagattttca tcccattcac gtagctgttt cttgattctg tccattaagg
     1381 tctcagcatc atataaggaa tacagccagc gaggccatga agttagattt gcacaatgaa
     1441 actttctctt ctggtatttc tgccaccatt tatatgaaca ttggtcttct cttgagacag
     1501 gttttgaagg aaatatctgg cacttattga gggattctaa ttgaactgca gacatggttg
     1561 aaggcaacac acattcggga agaatttgca ctttagcttg ctggattcca tctgactgtg
     1621 ttcttagctc aaggactttg aacctttgtc ttccaattgc tttcactttc actatctcaa
     1681 ttccaaaatc ctgttcttct cgataggcat atatctctgc tgttgttcca aactgtgctt
     1741 ccctttcctg tacattgctg tatgcaagaa cagcaaaggt tctatctttc tgaattaaat
     1801 tccgcaccat actgacttct tgagggtgaa aaagctgaag aggtaatgtc tgtccgggaa
     1861 tcaggatcat catcacttgt ggaagaactg gaatcacctg acagctgtcg tcatcgtgca
     1921 aagtcctgcc atgaaattct tccatatcag cacctaggta tgtatgtgat gtcggcagac
     1981 tggtgtcaaa atttatgatg tttggttttt tggcttcttt actatcctgg tcttcaactt
     2041 ccatttcatc ttcttcctca ctctctgcag gcaggagcgg caggtggttg cccatgttgt
     2101 gcgcagcgtc ctgctgatct ccttcgccgg ccatggcgat cgctagctgc aaaaagaaca
     2161 agtagcttgt attctatagt gtcacctaaa tgggcgatct gacggttcac taaaccagct
     2221 ctgcttatat aggcctccct ggcgcgccac tagttattaa tagtaatcaa ttacggggtc
     2281 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2461 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2521 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2581 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2641 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2701 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2761 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtcaaatcga attctaatac
     2821 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2881 ctaaacccag gaggcagatc atggagaaaa aaatcactgg atataccacc gttgatatat
     2941 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     3001 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     3061 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     3121 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     3181 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     3241 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     3301 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     3361 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcactatgg
     3421 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     3481 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     3541 agtggcaggg cggggcgtaa gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     3601 caaccggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3721 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4141 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     4201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4261 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa
     4321 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4381 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt atagtccgga
     4441 atcgtcacac aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc
     4501 atcgtgatgg ctagagttca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     4561 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     4621 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     4681 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     4741 gcatcgccgt gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     4801 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     4861 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     4921 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     4981 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     5041 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     5101 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     5161 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     5221 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     5281 gaacctgcgt gcaatccatc ttgttcaatc atggtggcat cgattcccgg tcaacatcca
     5341 gtacacctcc ggcaccaccg gcttccccaa gggcgccacg ctctcccacc acaacatcct
     5401 caacaacggc tactgggtgg gccgcacggt cggctacacg gagcaggacc gggtgtgtct
     5461 gccggtgccc ttctaccact gcttcggcat ggtcatgggc aacttgggcg ccacctccca
     5521 cgccgcctgc atcgtcatcc cggccccgtc cttcgagccg gcggcgacgc tggaggcggt
     5581 gcagcgggag aggtgcacgt ccctgtacgg cgtccccacg aagccgaatt ccaccacact
     5641 ggactagatg catgctcgac agtgtgatgg atatctgcag aattcggctt tcacgacacc
     5701 tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca
     5761 aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca
     5821 gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa
     5881 gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc
     5941 gtggagtcac atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac
     6001 tcacccgccc gctctcccga gcgccgcgtc ctccattttg agctgcctgc agcagggccg
     6061 ggaagcggcc atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag
     6121 ggcggggcga tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac
     6181 tacccccgtc cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc
     6241 tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga
     6301 tcttggcccg catttacaag actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa
     6361 aaattttaaa tggctagaga cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag
     6421 attcgcacgc ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg
     6481 gctccttaag cgcaaggcct cgaactctcc cacccacttc caacccgaag ctcgggatca
     6541 agaatcacgt actgcagcca ggtggaagta attcaaggca cgcaagggcc ataacccgta
     6601 aagaggccag gcccgcggga accacacacg gcacttacct gtgttctggc ggcaaacccg
     6661 ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg
     6721 ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc
     6781 taggcaccgg ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg
     6841 cgctctgccc actgacgggc accggagcc
//
