LOCUS       CDK2-SmBiT              5196 bp ds-DNA     circular     25-APR-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: CDK2-SmBiT.gb
            Sequence 'CDK2-SmBiT' generated by SeqNinja on Sep 27, 2022 at
            5:46:53 PM CDT. Equivalent SeqNinja expression: "H:\Seq\Benchling
            bucket\CDK2-SmBiT.sbd"#"CDK2-SmBiT"
FEATURES             Location/Qualifiers
     misc_feature    complement(22..209)
                     /label="SV40\polyA\signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /dnas_type="SV40\polyA\signal"
     misc_feature    complement(213..275)
                     /label="MCS 2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /dnas_type="MCS 2"
     promoter        276..866
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Has EcoICRI site removed. Named changed from ""CMVPromoter"" to ""CMV immediate-early enhancer/promoter""3/21/2011 and J Hartnet's request to fit Sci Com'snaming."
                     /dnas_type="CMV promoter"
     intron          981..1113
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="Note that this is the actual sequence spliced out.The 5' donor site includes a 5' GA and the 3' acceptorsite adds a 3'G."
                     /dnas_type="chimeric intron"
     promoter        1157..1176
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /dnas_type="T7 promoter"
     misc_feature    1180..1187
                     /label="SgfI Site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Used in Flexi Vector Cloning"
                     /dnas_type="SgfI Site"
     misc_feature    1189..2082
                     /label="CDK2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /dnas_type="CDK2"
     stem_loop       2092..2136
                     /label="15GS linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /translation="SSSGGGGSGGGSSGG"
                     /dnas_type="15GS linker"
     gene            2137..2169
                     /label="SmBiT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /translation="VTGYRLFEEIL"
                     /dnas_type="SmBiT"
     polyA_signal    2250..2471
                     /label="Sv40 late polyadenylation signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /dnas_type="Sv40 late polyadenylation signal"
     misc_feature    complement(2495..2528)
                     /label="Cre site"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /dnas_type="Cre site"
     promoter        2537..2603
                     /label="EM7 bacterial promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /dnas_type="EM7 bacterial promoter"
     CDS             2617..3411
                     /label="KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Name changed frm ""Neomycin phosphotransferase""to ""Neomycin phosphotransferase coding region"" on3/21/2011 at Jim Hartnet's request to fit Sci Com'snaming."
                     /dnas_type="KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     rep_origin      3566..3602
                     /label="ColE1-derived plasmid replication origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="The actual origin is the first base."
                     /dnas_type="ColE1-derived plasmid replication origin"
     polyA_signal    complement(4284..4331)
                     /label="Synthetic PolyAdenylation Signal Sequence (frompCiNeo vectors)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /dnas_type="Synthetic PolyAdenylation Signal"
     misc_feature    complement(4355..4753)
                     /label="BlastR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /dnas_type="BlastR"
     misc_feature    complement(4778..5196)
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /dnas_type="SV40 promoter"
ORIGIN
        1 AAGACGCGTT AATTAAgacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccGCGGCCGC aataCGTCGA CGttatCAGC
      241 TGacttCGTA CGagagCCTA GGATTATGGC GCGCCACTAG TTATTAATAG TAATCAATTA
      301 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG
      361 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
      421 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
      481 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA
      541 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA
      601 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT
      661 ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG
      721 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA
      781 ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
      841 GAGCTGGTTT AGTGAACCGT CAGATCACTA GAAGCTTTAT TGCGGTAGTT TATCACAGTT
      901 AAATTGCTAA CGCAGTCAGT GCTTCTGACA CAACAGTCTC GAACTTAAGC TGCAGAAGTT
      961 GGTCGTGAGG CACTGGGCAG GTAAGTATCA AGGTTACAAG ACAGGTTTAA GGAGACCAAT
     1021 AGAAACTGGG CTTGTCGAGA CAGAGAAGAC TCTTGCGTTT CTGATAGGCA CCTATTGGTC
     1081 TTACTGACAT CCACTTTGCC TTTCTCTCCA CAGGTGTCCA CTCCCAGTTC AATTACAGCT
     1141 CTTAAGGCTA GAGTATTAAT ACGACTCACT ATAGGGCTAG CGATCGCCAT GGAGAACTTC
     1201 CAAAAGGTGG AAAAGATCGG AGAGGGCACG TACGGAGTTG TGTACAAAGC CAGAAACAAG
     1261 TTGACGGGAG AGGTGGTGGC GCTTAAGAAA ATCCGCCTGG ACACTGAGAC TGAGGGTGTG
     1321 CCCAGTACTG CCATCCGAGA GATCTCTCTG CTTAAGGAGC TTAACCATCC TAATATTGTC
     1381 AAGCTGCTGG ATGTCATTCA CACAGAAAAT AAACTCTACC TGGTTTTTGA ATTTCTGCAC
     1441 CAAGATCTCA AGAAATTCAT GGATGCCTCT GCTCTCACTG GCATTCCTCT TCCCCTCATC
     1501 AAGAGCTATC TGTTCCAGCT GCTCCAGGGC CTAGCTTTCT GCCATTCTCA TCGGGTCCTC
     1561 CACCGAGACC TTAAACCTCA GAATCTGCTT ATTAACACAG AGGGGGCCAT CAAGCTAGCA
     1621 GACTTTGGAC TAGCCAGAGC TTTTGGAGTC CCTGTTCGTA CTTACACCCA TGAGGTGGTG
     1681 ACCCTGTGGT ACCGAGCTCC TGAAATCCTC CTGGGCTGCA AATATTATTC CACAGCTGTG
     1741 GACATCTGGA GCCTGGGCTG CATCTTTGCT GAGATGGTGA CTCGCCGGGC CCTATTCCCT
     1801 GGAGATTCTG AGATTGACCA GCTCTTCCGG ATCTTTCGGA CTCTGGGGAC CCCAGATGAG
     1861 GTGGTGTGGC CAGGAGTTAC TTCTATGCCT GATTACAAGC CAAGTTTCCC CAAGTGGGCC
     1921 CGGCAAGATT TTAGTAAAGT TGTACCTCCC CTGGATGAAG ATGGACGGAG CTTGTTATCG
     1981 CAAATGCTGC ACTACGACCC TAACAAGCGG ATTTCGGCCA AGGCAGCCCT GGCTCACCCT
     2041 TTCTTCCAGG ATGTGACCAA GCCAGTACCC CATCTTCGAC TCGTTTCTCa aggcAGTTCA
     2101 GGTGGTGGCG GGAGCGGAGG TGGAGGctcg AGCGGTGTGA CCGGCTACCG GCTGTTCGAG
     2161 GAGATTCTGT AAGGCCGCGA CTCTAGAGTC GACCTGCAGG CATGCAAGCT GATTTGGCTG
     2221 CTGCCACCGC TGAGCAAGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG
     2281 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT
     2341 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA
     2401 TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT TTAAGCAAGT AAAACCTCTA
     2461 CAAATGTGGT AAAATCTTAA TTAAGAATTC TAATATAACT TCGTATAGCA TACATTATAC
     2521 GAAGTTATGG ATCCACTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT
     2581 ACGACAAGGT GAGGAACTAA ACCCAGGAGG CAGATCATGA TTGAACAAGA TGGATTGCAC
     2641 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
     2701 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
     2761 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG
     2821 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
     2881 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
     2941 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
     3001 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
     3061 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
     3121 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
     3181 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
     3241 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT
     3301 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT
     3361 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG ATTCGAAATG
     3421 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
     3481 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
     3541 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
     3601 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     3661 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     3721 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
     3781 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     3841 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     3901 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     3961 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG
     4021 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     4081 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     4141 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     4201 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     4261 TCCTTTTATA GTCCGGAATC GTCACACAAA AAACCAACAC ACAGATGTAA TGAAAATAAA
     4321 GATATTTTAT TGCGGCCATC GTGATGGCTA GAGTTTAGCC CTCCCACACA TAACCAGAGG
     4381 GCAGCAATTC ACGAATCCCA ACTGCCGTCG GCTGTCCATC ACTGTCCTTC ACTATGGCTT
     4441 TGATCCCAGG ATGCAGATCG AGAAGCACCT GTCGGCACCG TCCGCAGGGG CTCAAGATGC
     4501 CCCTGTTCTC ATTTCCGATC GCGACGATAC AAGTCAGGTT GCCAGCTGCC GCAGCAGCAG
     4561 CAGTGCCCAG CACCACGAGT TCTGCACAAG GTCCCCCAGT AAAATGATAT ACATTGACAC
     4621 CAGTGAAGAT GCGGCCGTCG CTAGAGAGAG CTGCGCTGGC GACGCTGTAG TCTTCAGAGA
     4681 TGGGGATGCT GTTGATTGTA GCCGTTGCTC TTTCAATGAG GGTGGATTCT TCTTGAGACA
     4741 AAGGCTTGGC CATggtggcg ctagtgtcag aagaatcGAG CTTTTTGCAA AAGCCTAGGC
     4801 CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT
     4861 CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG
     4921 TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA
     4981 TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT
     5041 AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC
     5101 CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA GAAGGTACCT AACCAAGTTC
     5161 CTCTTTCAGA GGTTATTTCA GGCCATGGTG CTGCGC
//