LOCUS       Exported                4597 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pNB6k designed by Brock F. Binkowski

            

            Amplify pNBe5 w/ NB76/77 (800 bp); digest PCR product and pNB2k w/ 
            BglII & NheI (buffer B); purify, ligate and transform BR610 via 
            electroporation.
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..753
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    793..800
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    802..1362
                     /label=CYBC1
                     /note="CYBC1"
     misc_feature    1367..1462
                     /label=Untitled
     CDS             1372..1416
                     /codon_start=1
                     /label=15GS linker
                     /note="15GS linker"
                     /translation="GSSGGGGSGGGGSSG"
     CDS             1417..1449
                     /codon_start=1
                     /label=114 complementation peptide
                     /note="114 complementation peptide"
                     /translation="VTGYRLFEEIL"
     polyA_signal    1586..1807
                     /label=Sv40 late polyadenylation signal
     promoter        1906..2324
                     /label=SV40 promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        2332..2398
                     /label=EM7 bacterial promoter
     CDS             2412..3206
                     /label=NeoR / KanR
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    3270..3318
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      3554..3590
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 aaatgagtct tcggacctcg cgggggccgc ttaagcggtg gttagggttt gtctgacgcg
       61 gggggagggg gaaggaacga aacactctca ttcggaggcg gctcggggtt tggtcttggt
      121 ggccacgggc acgcagaaga gcgccgcgat cctcttaagc acccccccgc cctccgtgga
      181 ggcgggggtt tggtcggcgg gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc
      241 cccagagtgt gaccttttcg gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg
      301 acgggctcgc tgggtcctag gctccatggg gaccgtatac gtggacaggc tctggagcat
      361 ccgcacgact gcggtgatat taccggagac cttctgcggg acgagccggg tcacgcggct
      421 gacgcggagc gtccgttggg cgacaaacac caggacgggg cacaggtaca ctatcttgtc
      481 acccggaggc gcgagggact gcaggagctt cagggagtgg cgcagctgct tcatccccgt
      541 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
      601 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaagtcga acacgcagat
      661 gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
      721 gaacaccgag cgaccctgca gcgacccgct taaaagcttg gcaatccggt actgttggta
      781 aagccaccgc tagcgatcgc catgtacctg caggtggaga cccgcaccag ctcccgcctc
      841 catctgaaga gggctccagg catccggtcc tggtccctgc tggttggaat cttgtcgatt
      901 ggcctggctg ctgcctacta cagcggagat agcctgggct ggaagctctt ctacgtcaca
      961 ggctgcctgt ttgtggctgt gcagaacttg gaggactggg aggaagccat cttcgacaag
     1021 agcacaggga aggttgtttt gaagacgttc agcctctaca agaagctgct gactcttttc
     1081 agagctggcc acgaccaggt ggtggtcctg ctccatgatg tccgtgatgt gagcgtggag
     1141 gaggagaagg tccggtactt cgggaaaggc tacatggtgg tgctccggct tgcgacgggc
     1201 ttctcccacc ccctcacgca gagtgcagtc atgggccacc gcagtgatgt ggaagccatc
     1261 gccaagctca tcaccagctt cctggagctg cactgccttg agagccccac agagctgtct
     1321 cagagcagcg acagtgaggc cggtgaccct gcaagccaga gcgtttctca aggcagttca
     1381 ggtggtggcg ggagcggagg tggaggctcg agcggtgtga ccggctaccg gctgttcgag
     1441 gagattctgt aaggccgcga ctctagagtc gacctgcagg catgcaagct gatccggctg
     1501 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     1561 aaccccttgg ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     1621 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     1681 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     1741 atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa
     1801 tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     1861 ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc
     1921 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
     1981 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     2041 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     2101 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     2161 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2221 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2281 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaattaa ctgttgacaa
     2341 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccca
     2401 ggaggcagat catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     2461 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     2521 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     2581 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     2641 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     2701 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     2761 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     2821 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     2881 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     2941 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3001 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3061 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3121 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3181 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     3241 gacgcccaac ctgccatcac gatggccgca ataaaatatc tttattttca ttacatctgt
     3301 gtgttggttt tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt gcgttttccc
     3361 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     3421 ctttctaccc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4141 ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt
     4261 ccggaaatac aggaacgcac gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa
     4321 tggcagcttc agtggattaa gtgggggtaa tgtggcctgt accctctggt tgcataggta
     4381 ttcatacggt taaaatttat caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca
     4441 tttttacctg tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag
     4501 ggattatggt aaatccactt actgtctgcc ctcgtagcca tcgagataaa ccgcagtact
     4561 ccggccacga tgcgtccggc gtagaggatc gagatct
//
