LOCUS       Exported                9165 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pRSG233 aka CS1603B163
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(22..209)
                     /label=SV40\polyA\signal
     misc_feature    181..247
                     /label=Untitled
     misc_feature    215..281
                     /label=Untitled
     gene            complement(285..758)
                     /label=LgBiT
                     /note="LgBiT"
     misc_feature    798..3095
                     /label=BRAF
                     /note="BRAF"
     misc_feature    complement(3097..3104)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3109..3135
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(3136..3152)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(3155..3200)
                     /label=Minimal CMV Promoter 2
     misc_feature    3178..3200
                     /label=core
                     /note="core"
     promoter        3210..3800
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          3915..4047
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        4091..4110
                     /label=T7 promoter
     misc_feature    4114..4121
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4123..6066
                     /label=CRAF
     misc_feature    6076..6105
                     /label=10GS Linker
                     /note="10GS Linker"
     CDS             6106..6138
                     /codon_start=1
                     /label=SmBiT
                     /note="SmBiT"
                     /translation="VTGYRLFEEIL"
     polyA_signal    6219..6440
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(6464..6497)
                     /label=Cre site
                     /note="Cre site"
     promoter        6506..6572
                     /label=EM7 bacterial promoter
     CDS             6586..7380
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    7385..7424
                     /label=Untitled
     rep_origin      7535..7571
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(8253..8300)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(8324..8722)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(8747..9165)
                     /label=SV40 promoter
                     /note="SV40 promoter"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggtgtttcta gagtcgcggc cttagctgtt gatggttact
      301 cggaacagca tggagccgtc gggggtgatc aggcgctcgt cgataatttt gttgccgttc
      361 cacagggtcc ctgttacagt gatctttttg ccgtcgaaca cggcgatgcc ttcatacggc
      421 cgtccgaaat agttcagcat gttcggcgta accccgtcga ttaccagtgt gccatagggc
      481 aggatcacct taaagtgatg atcatccaca gggtacacca ccttaaacac ctcttcgatc
      541 tgggccattt ggtcggcgct cagaccttca tacgggatga tgacatggat gtcgatcttc
      601 agggcatttt caccgctccg gacaatcctt tggatcggag ttacggacac ggcgagattc
      661 tgcagcaaac tggacacacc tccctgttca aggacttggt ccaggttgta ggcggctgtc
      721 tgttcccagt ccccaacgaa atcttcgagt gtgaagacgg agccccctcc acctgagcca
      781 cctcctccga gagaaacgtg gacaggaaac gcaccatatc cccctgcctg gatgggtgtt
      841 tttggagaag cacaagcata tagactaaaa tcctctgttt ggaaaccagc ccgattcaag
      901 gagggttctg atgcactgcg gtgaattttt ggcaatgagc gggccagcag ctcaatagag
      961 gcgagaattt ggggaaagag tggtctctca tctcttttct ttttgaggca ctctgccatt
     1021 aatctcttca tggcttttgg acagttactc cgtaccttac tgagatctgg agacaggtat
     1081 cctcgtccca ccataaaaat tatctggtcc ctgttgttga tgtttgaata aggtaactgt
     1141 ccagtcatca attcatacag aacaattcca aatgcatata catctgactg aaagctgtat
     1201 ggatttttat cttgcattct gatgacttct ggtgccatcc acaaaatgga tccagacaac
     1261 tgttcaaact gatgggaccc actccatcga gatttcactg tagctagacc aaaatcacct
     1321 atttttactg tgaggtcttc atgaagaaat atattattac tcttgaggtc tctgtggatg
     1381 attgacttgg cgtgtaagta atccatgccc tgtgcagtct gtcgtgcaat atctataagt
     1441 ttgatcatct caaatttggt ctcaatgata tggagatggt gatacaagct ggagccctca
     1501 caccactggg taacaatagc cagttgtggc tttgtggaat agcccatgaa gagtaggata
     1561 ttcacatgtc gtgttttcct gagtactcct acttcatttt tgaaggcttg taactgctga
     1621 ggtgtaggtg ctgtcacatt caacattttc actgccacat caccatgcca ctttcccttg
     1681 tagactgttc caaatgatcc agatccaatt ctttgtccca ctgtaatctg cccatcagga
     1741 atctcccaat catcactcga gtcccgtcta ccaagtgttt tcattcgatt cctgtcttct
     1801 gaggatgaag atgacttcct ttctcgctga ggtcctggag atttctgtaa ggctttcacg
     1861 ttagttagtg agccaggtaa tgaggcaggg ggggtagcag acaaacctgt ggttgatcct
     1921 ccatcaccac gaaatccttg gtctctaatc aagtcatcaa tattgacagg ttctattgtg
     1981 tttatatgca cattgggagc tgatgaggat cggtctcgtt gcccaaattg atttcgatga
     2041 tcttcatctg ctggtcggaa gggctgtgga attggaatgg attttgaagg agacggactg
     2101 gtgagaattt ggggcccaat agagtccgag gcgggtgcgg aaggggatga tccagatgtt
     2161 agggcagtct ctgctaagga cgcctcttcc tgtggtattg ggtggtgttc aaagaacttg
     2221 gagacaaaca gcaaatcaag ttggtcataa ttaacacaca tcagtggaac ttctgtacta
     2281 caacgctggt gaaatttata accacatgtt tgacagcgga aaccctggaa aagcagcttt
     2341 cgacaaaagt cacaaaatgc taaggtgaaa aacgtttttc gtacaaagtt gtgtgttgta
     2401 agtggaacat tctccaacac ttccacatgc aattcttctc cagtaagcca ggaaatatca
     2461 gtgtcccaac caattggttt cttctctcca tcctgaattc tgtaaacagc acagcactct
     2521 gggattagac ctctcatcat cagtgctttc tttagactgt ctcggactgt aactccacac
     2581 cttgcaggta ccactgtcct ctgtttgttg ggcaggaaga ctctaacgat aggtttttgt
     2641 ggtgacttgg ggttgctccg tgccacatct gtgggatttt gaaaaactga aagagatgaa
     2701 ggtagcactg aaaggctaga agaggaagaa gatgtaacgg tatccattga tgcagagcta
     2761 gaaacagaaa aatcagttcc gttccccaga gattccaata actgttgttc tctttgttgg
     2821 agtgcatcta gcttgctggt gtattcttca taggcctcca gatatattga tggtggatta
     2881 tgctccccac caaatttgtc caatagggcc tctatatgtt cctgtgtcaa cttaatcatt
     2941 tgtttgatat tccacacctc ctccggaatg gcagggtccg cagccgaaga ggccgcggcg
     3001 ccggcgccgg cgccggcctc gggctccatg tccccgttga acagagcctg gcccggctcc
     3061 gcgccgccac caccgccacc gctcagcgcc gccatggcga tcgctagctg caaaaagaac
     3121 aagtagcttg tattctatag tgtcacctaa atgggcgatc tgacggttca ctaaaccagc
     3181 tctgcttata taggcctccc tggcgcgcca ctagttatta atagtaatca attacggggt
     3241 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3301 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3361 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3421 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     3481 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     3541 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     3601 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     3661 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     3721 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
     3781 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg
     3841 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt
     3901 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
     3961 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
     4021 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag
     4081 gctagagtat taatacgact cactataggg ctagcgatcg ccatggagca catacaggga
     4141 gcttggaaga cgatcagcaa tggttttgga ttcaaagatg ccgtgtttga tggctccagc
     4201 tgcatctctc ctacaatagt tcagcagttt ggctatcagc gccgggcatc agatgatggc
     4261 aaactcacag atccttctaa gacaagcaac actatccgtg ttttcttgcc gaacaagcaa
     4321 agaacagtgg tcaatgtgcg aaatggaatg agcttgcatg actgccttat gaaagcactc
     4381 aaggtgaggg gcctgcaacc agagtgctgt gcagtgttca gacttctcca cgaacacaaa
     4441 ggtaaaaaag cacgcttaga ttggaatact gatgctgcgt ctttgattgg agaagaactt
     4501 caagtagatt tcctggatca tgttcccctc acaacacaca actttgctcg gaagacgttc
     4561 ctgaagcttg ccttctgtga catctgtcag aaattcctgc tcaatggatt tcgatgtcag
     4621 acttgtggct acaaatttca tgagcactgt agcaccaaag tacctactat gtgtgtggac
     4681 tggagtaaca tcagacaact cttattgttt ccaaattcca ctattggtga tagtggagtc
     4741 ccagcactac cttctttgac tatgcgtcgt atgcgagagt ctgtttccag gatgcctgtt
     4801 agttctcagc acagatattc tacacctcac gccttcacct ttaacacctc cagtccctca
     4861 tctgaaggtt ccctctccca gaggcagagg tcgacatcca cacctaatgt ccacatggtc
     4921 agcaccaccc tgcctgtgga cagcaggatg attgaggatg caattcgaag tcacagcgaa
     4981 tcagcctcac cttcagccct gtccagtagc cccaacaatc tgagcccaac aggctggtca
     5041 cagccgaaaa cccccgtgcc agcacaaaga gagcgggcac cagtatctgg gacccaggag
     5101 aaaaacaaaa ttaggcctcg tggacagaga gattcaagct attattggga aatagaagcc
     5161 agtgaagtga tgctgtccac tcggattggg tcaggctctt ttggaactgt ttataagggt
     5221 aaatggcacg gagatgttgc agtaaagatc ctaaaggttg tcgacccaac cccagagcaa
     5281 ttccaggcct tcaggaatga ggtggctgtt ctgcgcaaaa cacggcatgt gaacattctg
     5341 cttttcatgg ggtacatgac aaaggacaac ctggcaattg tgacccagtg gtgcgagggc
     5401 agcagcctct acaaacacct gcatgtccag gagaccaagt ttcagatgtt ccagctaatt
     5461 gacattgccc ggcagacggc tcagggaatg gactatttgc atgcaaagaa catcatccat
     5521 agagacatga aatccaacaa tatatttctc catgaaggct taacagtgaa aattggagat
     5581 tttggtttgg caacagtaaa gtcacgctgg agtggttctc agcaggttga acaacctact
     5641 ggctctgtcc tctggatggc cccagaggtg atccgaatgc aggataacaa cccattcagt
     5701 ttccagtcgg atgtctactc ctatggcatc gtattgtatg aactgatgac gggggagctt
     5761 ccttattctc acatcaacaa ccgagatcag atcatcttca tggtgggccg aggatatgcc
     5821 tccccagatc ttagtaagct atataagaac tgccccaaag caatgaagag gctggtagct
     5881 gactgtgtga agaaagtaaa ggaagagagg cctctttttc cccagatcct gtcttccatt
     5941 gagctgctcc aacactctct accgaagatc aaccggagcg cttccgagcc atccttgcat
     6001 cgggcagccc acactgagga tatcaatgct tgcacgctga ccacgtcccc gaggctgcct
     6061 gtcttcgttt ctctcggagg aggtggctca ggtggagggg gctccgtgac cggctaccgg
     6121 ctgttcgagg agattctgta aggccgcgac tctagagtcg acctgcaggc atgcaagctg
     6181 atttggctgc tgccaccgct gagcaaggcc gcttcgagca gacatgataa gatacattga
     6241 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     6301 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     6361 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
     6421 aaacctctac aaatgtggta aaatcttaat taagaattct aatataactt cgtatagcat
     6481 acattatacg aagttatgga tccactgttg acaattaatc atcggcatag tatatcggca
     6541 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     6601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     6781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6961 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7021 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7081 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     7141 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7201 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7261 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7321 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7381 ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag gcggtaatac
     7441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8101 ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg
     8161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8221 tcacctagat ccttttatag tccggaatcg tcacacaaaa aaccaacaca cagatgtaat
     8281 gaaaataaag atattttatt gcggccatcg tgatggctag agtttagccc tcccacacat
     8341 aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca
     8401 ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc
     8461 tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg
     8521 cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata
     8581 cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt
     8641 cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt
     8701 cttgagacaa aggcttggcc atggtggcgc tagtgtcaga agaatcgagc tttttgcaaa
     8761 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     8821 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     8881 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     8941 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     9001 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     9061 ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag aaggtaccta
     9121 accaagttcc tctttcagag gttatttcag gccatggtgc tgcgc
//
