LOCUS       Exported                6264 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       UnnamedSequence      5152 bp    DNA   circular          
             9/13/2011

            DEFINITION  Current Name: Unnamed Sequence
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="synthetic DNA construct"
                     /label=pNB1k
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from %22CMV 
                     Promoter%22 to %22CMV immediate-early enhancer/promoter%22 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     misc_feature    1056..1060
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..2597
                     /label=EPOR
                     /note="EPOR"
     CDS             2598..2642
                     /codon_start=1
                     /label=15GS linker
                     /note="15GS linker"
                     /translation="GSSGGGGSGGGGSSG"
     gene            2643..3116
                     /label=11S complementation fragment
                     /note="11S complementation fragment"
     polyA_signal    3253..3474
                     /label=Sv40 late polyadenylation signal
     promoter        3573..3991
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(3646..3882)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      3889..3954
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        3999..4065
                     /label=EM7 bacterial promoter
     CDS             4079..4873
                     /label=NeoR / KanR
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    4937..4985
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5221..5257
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    5928..6213
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggac cacctcgggg
     1081 cgtccctctg gccccaggtc ggctcccttt gtctcctgct cgctggggcc gcctgggcgc
     1141 ccccgcctaa cctcccggac cccaagttcg agagcaaagc ggccttgctg gcggcccggg
     1201 ggcccgaaga gcttctgtgc ttcaccgagc ggttggagga cttggtgtgt ttctgggagg
     1261 aagcggcgag cgctggggtg ggcccgggca actacagctt ctcctaccag ctcgaggatg
     1321 agccatggaa gctgtgtcgc ctgcaccagg ctcccacggc tcgtggtgcg gtgcgcttct
     1381 ggtgttcgct gcctacagcc gacacgtcga gcttcgtgcc cctagagttg cgcgtcacag
     1441 cagcctccgg cgctccgcga tatcaccgtg tcatccacat caatgaagta gtgctcctag
     1501 acgcccccgt ggggctggtg gcgcggttgg ctgacgagag cggccacgta gtgttgcgct
     1561 ggctcccgcc gcctgagaca cccatgacgt ctcacatccg ctacgaggtg gacgtctcgg
     1621 ccggcaacgg cgcagggagc gtacagaggg tggagatcct ggagggccgc accgagtgtg
     1681 tgctgagcaa cctgcggggc cggacgcgct acaccttcgc cgtccgcgcg cgtatggctg
     1741 agccgagctt cggcggcttc tggagcgcct ggtcggagcc tgtgtcgctg ctgacgccta
     1801 gcgacctgga ccccctcatc ctgacgctct ccctcatcct cgtggtcatc ctggtgctgc
     1861 tgaccgtgct cgcgctgctc tcccaccgcc gggctctgaa gcagaagatc tggcctggca
     1921 tcccgagccc agagagcgag tttgaaggcc tcttcaccac ccacaagggt aacttccagc
     1981 tgtggctgta ccagaatgat ggctgcctgt ggtggagccc ctgcaccccc ttcacggagg
     2041 acccacctgc ttccctggaa gtcctctcag agcgctgctg ggggacgatg caggcagtgg
     2101 agccggggac agatgatgag ggccccctgc tggagccagt gggcagtgag catgcccagg
     2161 atacctatct ggtgctggac aaatggttgc tgccccggaa cccgcccagt gaggacctcc
     2221 cagggcctgg tggcagtgtg gacatagtgg ccatggatga aggctcagaa gcatcctcct
     2281 gctcatctgc tttggcctcg aagcccagcc cagagggagc ctctgctgcc agctttgagt
     2341 acactatcct ggaccccagc tcccagctct tgcgtccatg gacactgtgc cctgagctgc
     2401 cccctacccc accccaccta aagtacctgt accttgtggt atctgactct ggcatctcaa
     2461 ctgactacag ctcaggggac tcccagggag cccaaggggg cttatccgat ggcccctact
     2521 ccaaccctta tgagaacagc cttatcccag ccgctgagcc tctgcccccc agctatgtgg
     2581 cttgctctgt ttctcaaggc agttcaggtg gtggcgggag cggaggtgga ggctcgagcg
     2641 gtgtcttcac actcgaagat ttcgttgggg actgggaaca gacagccgcc tacaacctgg
     2701 accaagtcct tgaacaggga ggtgtgtcca gtttgctgca gaatctcgcc gtgtccgtaa
     2761 ctccgatcca aaggattgtc cggagcggtg aaaatgccct gaagatcgac atccatgtca
     2821 tcatcccgta tgaaggtctg agcgccgacc aaatggccca gatcgaagag gtgtttaagg
     2881 tggtgtaccc tgtggatgat catcacttta aggtgatcct gccctatggc acactggtaa
     2941 tcgacggggt tacgccgaac atgctgaact atttcggacg gccgtatgaa ggcatcgccg
     3001 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg
     3061 acgagcgcct gatcaccccc gacggctcca tgctgttccg agtaaccatc aacagctaag
     3121 gccgcgactc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg
     3181 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     3241 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3301 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3361 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3421 agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg
     3481 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     3541 tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc
     3601 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     3661 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3721 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3781 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3901 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3961 ttttggaggc ctaggctttt gcaaaaagct taattaactg ttgacaatta atcatcggca
     4021 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
     4081 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4141 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4201 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4261 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4321 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4381 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4441 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4501 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4561 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     4621 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4681 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4741 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4801 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4861 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4921 ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     4981 gtgtgaatcg atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc
     5041 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacccggt
     5101 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5641 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     5701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc
     5821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaaatacagg
     5941 aacgcacgct ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt
     6001 ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc ataggtattc atacggttaa
     6061 aatttatcag gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct
     6121 gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga ttatggtaaa
     6181 tccacttact gtctgccctc gtagccatcg agataaaccg cagtactccg gccacgatgc
     6241 gtccggcgta gaggatcgag atct
//
