LOCUS       Exported                6182 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     misc_feature    complement(805..808)
                     /label=PmeI
                     /note="PmeI"
     misc_feature    complement(807..1447)
                     /label=VHL
                     /note="VHL"
     misc_feature    complement(1449..1456)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1461..1487
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(1488..1504)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(1507..1552)
                     /label=Minimal CMV Promoter 2
     misc_feature    1530..1552
                     /label=core
                     /note="core"
     promoter        1562..2116
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2134..2200
                     /label=EM7 bacterial promoter
     CDS             2214..2873
                     /label=Chloramphenicol resistance
     rep_origin      3042..3078
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(3760..3807)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(3831..4625)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(4639..4933)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5004..6182)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacat ctcccatccg ttgatgtgca atgcgctcct
      841 gtgtcagccg ctccaggtct ttctgcacat ttgggtggtc ttccagatct tcgtagagcg
      901 acctgacgat gtccagtctc ctgtaattct caggcttgac taggctccgg acaacctgga
      961 ggcatcgctc tttcagagta tacactggca gtgtgatatt ggcaaaaata ggctgtccgt
     1021 caacattgag agatggcaca aataattcag tttggttaac cagaagccca tcgtgtgtcc
     1081 ctgcatctct gaagagccaa aggtgacctc ggtagctgtg gatgcggcgg cccgtgccag
     1141 gcggcagcgt tgggtagggc tgcggctcgc cgtcgaagtt gagccatacg ggcagcacga
     1201 cgcgcggact gcgattgcag aagatgacct gggagggctc gcgcgagttc accgagcgca
     1261 gcacgggccg cggccgcccg gcctccatct cctcctcggc gcccagttcc tccgggccgg
     1321 actcttccgg gccggactcc tcggcgcccg actcctcccc gccgtcttct tcagggccgt
     1381 actcttcgac gcctgcctcc tccgcgccta cctcggcctc gtcccagttc tccgccctcc
     1441 ggggcatggc gatcgctagc tgcaaaaaga acaagtagct tgtattctat agtgtcacct
     1501 aaatgggcga tctgacggtt cactaaacca gctctgctta tataggcctc cctggcgcgc
     1561 cactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1621 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1681 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1741 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1801 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1861 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1921 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     1981 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2041 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
     2101 tgtacggtgg gaggtcaaat cgaattctaa tactgttgac aattaatcat cggcatagta
     2161 tatcggcata gtataatacg acaaggtgag gaactaaacc caggaggcag atcatggaga
     2221 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     2281 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     2341 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     2401 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     2461 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     2521 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     2581 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     2641 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     2701 tggacaactt cttcgccccc gttttcacta tgggcaaata ttatacgcaa ggcgacaagg
     2761 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     2821 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taagcgggac
     2881 tctggggttc gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg
     2941 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3001 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3061 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3121 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3181 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3241 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3301 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3361 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3421 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3481 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3541 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3601 cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg
     3661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3721 aggatcttca cctagatcct tttatagtcc ggaatcgtca cacaaaaaac caacacacag
     3781 atgtaatgaa aataaagata ttttattgcg gccatcgtga tggctagagt tcagaagaac
     3841 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     3901 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     3961 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     4021 cggccatttt ccaccatgat attcggcaag caggcatcgc cgtgggtcac gacgagatcc
     4081 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     4141 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     4201 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     4261 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4321 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4381 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4441 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     4501 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     4561 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     4621 atcatggtgg catcgattcc cggtcaacat ccagtacacc tccggcacca ccggcttccc
     4681 caagggcgcc acgctctccc accacaacat cctcaacaac ggctactggg tgggccgcac
     4741 ggtcggctac acggagcagg accgggtgtg tctgccggtg cccttctacc actgcttcgg
     4801 catggtcatg ggcaacttgg gcgccacctc ccacgccgcc tgcatcgtca tcccggcccc
     4861 gtccttcgag ccggcggcga cgctggaggc ggtgcagcgg gagaggtgca cgtccctgta
     4921 cggcgtcccc acgaagccga attccaccac actggactag atgcatgctc gacagtgtga
     4981 tggatatctg cagaattcgg ctttcacgac acctgaaatg gaagaaaaaa actttgaacc
     5041 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag
     5101 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac
     5161 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac
     5221 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag
     5281 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc
     5341 gtcctccatt ttgagctgcc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca
     5401 actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg
     5461 ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc
     5521 gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc
     5581 gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct
     5641 tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg
     5701 aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca
     5761 ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc
     5821 tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa
     5881 gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac
     5941 acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact
     6001 actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca
     6061 tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct
     6121 ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag
     6181 cc
//
