LOCUS       Exported                6713 bp ds-DNA     circular SYN 30-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)-BRD4      8855 bp    DNA   circular    
                   7/11/2014

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) - BRD4 Old/Alternate 
            Name(s):BRD4 (insert) CAP (insert) HUNK1 (insert) HUNKI (insert) 
            MCAP (insert) pF-Nn5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     CDS             1065..3548
                     /label=Untitled
     CDS             1065..1577
                     /label=N terminal NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1594..1598
                     /label=BRD4 insert
                     /note="InsertComparisonToAccessionNumber updated to The 
                     sequence is identical to the CDS sequences from NM_058243. 
                     by Ethan Strauss on 9/26/2012. InsertPrimerInformation 
                     updated to Unknown by Ethan Strauss on 9/26/2012. 
                     Apparently ordered as custom by Danette (Note added on 
                     9/26/2012 by esadmin)"
     misc_feature    1599..3546
                     /label=CRAF insert
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:27:23 AM"
     misc_feature    3547..3680
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     misc_feature    3547..3550
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3702..3923
                     /label=Sv40 late polyadenylation signal
     promoter        4022..4440
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(4095..4331)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      4338..4403
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        4448..4514
                     /label=EM7 bacterial promoter
     CDS             4528..5322
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5386..5434
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5670..5706
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6377..6662
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggagcacata cagggagctt
     1621 ggaagacgat cagcaatggt tttggattca aagatgccgt gtttgatggc tccagctgca
     1681 tctctcctac aatagttcag cagtttggct atcagcgccg ggcatcagat gatggcaaac
     1741 tcacagatcc ttctaagaca agcaacacta tccgtgtttt cttgccgaac aagcaaagaa
     1801 cagtggtcaa tgtgcgaaat ggaatgagct tgcatgactg ccttatgaaa gcactcaagg
     1861 tgaggggcct gcaaccagag tgctgtgcag tgttcagact tctccacgaa cacaaaggta
     1921 aaaaagcacg cttagattgg aatactgatg ctgcgtcttt gattggagaa gaacttcaag
     1981 tagatttcct ggatcatgtt cccctcacaa cacacaactt tgctcggaag acgttcctga
     2041 agcttgcctt ctgtgacatc tgtcagaaat tcctgctcaa tggatttcga tgtcagactt
     2101 gtggctacaa atttcatgag cactgtagca ccaaagtacc tactatgtgt gtggactgga
     2161 gtaacatcag acaactctta ttgtttccaa attccactat tggtgatagt ggagtcccag
     2221 cactaccttc tttgactatg cgtcgtatgc gagagtctgt ttccaggatg cctgttagtt
     2281 ctcagcacag atattctaca cctcacgcct tcacctttaa cacctccagt ccctcatctg
     2341 aaggttccct ctcccagagg cagaggtcga catccacacc taatgtccac atggtcagca
     2401 ccaccctgcc tgtggacagc aggatgattg aggatgcaat tcgaagtcac agcgaatcag
     2461 cctcaccttc agccctgtcc agtagcccca acaatctgag cccaacaggc tggtcacagc
     2521 cgaaaacccc cgtgccagca caaagagagc gggcaccagt atctgggacc caggagaaaa
     2581 acaaaattag gcctcgtgga cagagagatt caagctatta ttgggaaata gaagccagtg
     2641 aagtgatgct gtccactcgg attgggtcag gctcttttgg aactgtttat aagggtaaat
     2701 ggcacggaga tgttgcagta aagatcctaa aggttgtcga cccaacccca gagcaattcc
     2761 aggccttcag gaatgaggtg gctgttctgc gcaaaacacg gcatgtgaac attctgcttt
     2821 tcatggggta catgacaaag gacaacctgg caattgtgac ccagtggtgc gagggcagca
     2881 gcctctacaa acacctgcat gtccaggaga ccaagtttca gatgttccag ctaattgaca
     2941 ttgcccggca gacggctcag ggaatggact atttgcatgc aaagaacatc atccatagag
     3001 acatgaaatc caacaatata tttctccatg aaggcttaac agtgaaaatt ggagattttg
     3061 gtttggcaac agtaaagtca cgctggagtg gttctcagca ggttgaacaa cctactggct
     3121 ctgtcctctg gatggcccca gaggtgatcc gaatgcagga taacaaccca ttcagtttcc
     3181 agtcggatgt ctactcctat ggcatcgtat tgtatgaact gatgacgggg gagcttcctt
     3241 attctcacat caacaaccga gatcagatca tcttcatggt gggccgagga tatgcctccc
     3301 cagatcttag taagctatat aagaactgcc ccaaagcaat gaagaggctg gtagctgact
     3361 gtgtgaagaa agtaaaggaa gagaggcctc tttttcccca gatcctgtct tccattgagc
     3421 tgctccaaca ctctctaccg aagatcaacc ggagcgcttc cgagccatcc ttgcatcggg
     3481 cagcccacac tgaggatatc aatgcttgca cgctgaccac gtccccgagg ctgcctgtct
     3541 tcgtttaaac gaattcgggc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
     3601 caagctgatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     3661 gagcaataac tagcataacc ccttggggcg gccgcttcga gcagacatga taagatacat
     3721 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3781 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3841 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt ttttaagcaa
     3901 gtaaaacctc tacaaatgtg gtaaaatcga attttaacaa aatattaacg cttacaattt
     3961 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgcggatc
     4021 tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg taccttctga
     4081 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4141 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4201 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4261 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4321 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4381 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     4441 aattaactgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     4501 tgaggaacta aacccaggag gcagatcatg attgaacaag atggattgca cgcaggttct
     4561 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4621 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4681 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4741 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4801 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4861 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4921 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4981 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5041 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5101 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5161 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5221 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5281 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5341 aaatgaccga ccaagcgacg cccaacctgc catcacgatg gccgcaataa aatatcttta
     5401 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagcgataag gatcctcttt
     5461 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     5521 cgtttctgcg gactggcttt ctacccggta tcagctcact caaaggcggt aatacggtta
     5581 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5641 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5701 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5761 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5821 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5881 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5941 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6001 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6061 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     6121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6241 cgcagaaaaa aaggatttca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6361 tagatccttt tatagtccgg aaatacagga acgcacgctg gatggccctt cgctgggatg
     6421 gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc
     6481 tctggttgca taggtattca tacggttaaa atttatcagg cgcgattgcg gcagtttttc
     6541 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc
     6601 ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga
     6661 gataaaccgc agtactccgg ccacgatgcg tccggcgtag aggatcgaga tct
//
